Hemostatic potential of latex proteases from Tabernaemontana divaricata (L.) R. Br. ex. Roem. and Schult. and Artocarpus altilis (Parkinson ex. F.A. Zorn) Forsberg

Singh, Maheshwari Kumari; Usha, R.; Hithayshree, K. R.; Sukumaran, Bindhu Omana

doi:10.1007/s11239-013-1012-y

Cited by 18 publications

(6 citation statements)

References 26 publications

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“…5 There are promising alternative approaches to the detection of BoNT that have been reviewed recently which include immunological methods, mass spectrometry, endopeptidase activity assays, and cell based assays. 6–8…”

Section: Introductionmentioning

confidence: 99%

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Jenko

Zhang

Kostenko

et al. 2014

Analyst

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Plant and microbial toxins are considered bioterrorism threat agents because of their extreme toxicity and/or ease of availability. Additionally, some of these toxins are increasingly responsible for accidental food poisonings. The current study utilized an ELISA-based protein antibody microarray for the multiplexed detection of ten biothreat toxins, botulinum neurotoxins (BoNT) A, B, C, D, E, F, ricin, shiga toxins 1 and 2 (Stx), and staphylococcus enterotoxin B (SEB), in buffer and complex biological matrices. The multiplexed assay displayed a sensitivity of 1.3 pg mL(-1) (BoNT/A, BoNT/B, SEB, Stx-1 and Stx-2), 3.3 pg mL(-1) (BoNT/C, BoNT/E, BoNT/F) and 8.2 pg mL(-1) (BoNT/D, ricin). All assays demonstrated high accuracy (75-120 percent recovery) and reproducibility (most coefficients of variation <20%). Quantification curves for the ten toxins were also evaluated in clinical samples (serum, plasma, nasal fluid, saliva, stool, and urine) and environmental samples (apple juice, milk and baby food) with overall minimal matrix effects. The multiplex assays were highly specific, with little cross-reactivity observed between the selected toxin antibodies. The results demonstrate a multiplex microarray that improves current immunoassay sensitivity for biological warfare agents in buffer, clinical, and environmental samples.

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Section: Introductionmentioning

confidence: 99%

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Jenko

Zhang

Kostenko

et al. 2014

Analyst

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“…There are several compounds present in plants which are responsible for AChE activity, like 19,20 dihydro tabernamine, conodurinbe, nitrogenmethyvofinine, conophylline etc. The method used for the activity is Ellman's method 71,[86][87][88][89][90][91] .…”

Section: Acetylcholinesterase Activitymentioning

confidence: 99%

<i>Tabernaemontana divaricata</i>: A Herbal Panacea

Das

Dubey

Divya

2022

JNR

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Tabernaemontana divaricata (pinwheel flower) is a flowering plant that can grow easily in gardens and along roadsides. This plant can be cultivated in every condition. No specific environmental condition is required for the growth of the plant. Growing evidence suggests that this plant has medicinal benefits for various diseases due to the presence of bioactive components in the plant. The plant is extensively found near Indian heritage to be used for worship. T. divaricata contains major alkaloids like apparicine, conophylline, coronardine, ibogamine, etc., exhibiting pharmacological activities. Their major pharmacological potential is against inflammation, pain, and other diseases. Plants’ major activities, such as anti-diabetic, anti-inflammatory, antibacterial, antifungal, and so on, have been demonstrated by their responsible bioactive compounds. The review is to highlight the researchers’ findings of different medicinal activities in T. divaricata along with the major responsible phytocomponents. There is a lot more scope for further research, which can be extended by the help of this review.

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“…The flavonoids from this and other species have also been shown to have anti-inflammatory [5], anti-oxidative [7], hemostatic [8], anti-hypertensive [9], antihypercholesterolemic [10] and anti-atherosclerotic activities [11].…”

Section: Introductionmentioning

confidence: 95%

Molecular Mechanism of Action for the Geranyl Flavonoid to Counter Dyslipidemia in Diabetic Milieu

Niu¹,

Li²,

Ma³

et al. 2015

JFNR

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This work was to examine the effect of 3'-methyl-4', 7-dihydroxyflavanone, a geranyl flavonoid (GF), on hepatocellular AMPK activity and lipid levels in HepG2 cells and diabetic mice, and identify molecular mechanism of the GF action on remedying dyslipidemia. The AMPK activation and lipid-lowering effect of the GF in diabetic mice and in HepG2 cells paralleled observations. The GF activated AMPK in HepG2 cells treated with high glucose, and enhance phosphorylation of ACC1 and ACC2, two isoforms of ACC, resulting in decrease in ACC activity and hepatic lipids. As demonstrated in cells overexpressing a dominant-negative AMPK mutant, the effect of GF was shown to be mediated by the activation of AMPK. The AMPK was activated relatively rapidly by GF and well before any potential change in adenosine triphosphate (ATP) level was detected. Thus, both in vivo and in vitro inhibition of AMPK, activation of ACC, and hepatocellular lipid accumulation caused by sustained high glucose levels was effectively counteracted by activating AMPK with treatment of GF. It can be conclude that GF lower lipids both in vivo and in vitro by activating AMPK and inactivating ACC, and consequently down-regulating fatty acid synthesis. The work provides a strong evidence for GF as a new therapeutic agent to definitively remedy dyslipidemia in diabetic milieu.

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Hemostatic potential of latex proteases from Tabernaemontana divaricata (L.) R. Br. ex. Roem. and Schult. and Artocarpus altilis (Parkinson ex. F.A. Zorn) Forsberg

Cited by 18 publications

References 26 publications

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

<i>Tabernaemontana divaricata</i>: A Herbal Panacea

Molecular Mechanism of Action for the Geranyl Flavonoid to Counter Dyslipidemia in Diabetic Milieu

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