Hemostatic Efficacy of Biological Self‐Assembling Peptide Nanofibers in a Rat Kidney Model

Abstract

We evaluated the hemostatic efficacy of a biological self-assembling peptide RADA16-I in a rat kidney injury model. Adult male rats were randomized into five groups: sham operation (no renal excision), no hemostatic agent (control), commercially available gelatin sponge (Gelfoam), 1% RADA16-I, and 2% RADA16-I. After left partial nephrectomy, the anesthetized animal was anticoagulated using 300 IU x kg(-1) heparin, and the topical hemostatic agent was applied to the injury. Blood loss and mean arterial pressure (MAP) were recorded. As was the case for Gelfoam, 2% RADA16-I produced marked hemostasis versus controls (p < 0.01). Blood loss with 1% and 2% RADA16-I was significantly less than controls. The decline in MAP during surgery was less with 2% versus 1% RADA16-I. RADA16-I also resulted in less histological tissue responses than Gelfoam. These data suggest that RADA16-I can stop hemorrhage, with only minimal tissue responses, in experimental renal injury.

The Neutral Self-Assembling Peptide Hydrogel SPG-178 as a Topical Hemostatic Agent

“…3). Macrophage infiltration and some foreign body giant cells surrounding both SPG-178 and RADA16 remnants were observed, and these images were similar to those in a previous study [11]. A possible cause for this observation is that the contact time between the liver and hydrogel was short and the remnants were very few.…”

“…The complete sequence of a self-assembling peptide was originally found in a region of alternating hydrophobic and hydrophilic residues in a yeast protein, zuotin [8], which was characterized by a stable β-sheet structure and known to undergo self-assembly into nanofibers that could further form a hydrogel [9], [10]. Self-assembling peptide hydrogels have already been employed for topical hemostasis [7], [11]–[13], tissue engineering [14]–[17], drug delivery systems [18], [19], and wound healing [20], [21].…”

Design of Self-Assembling Peptides and Their Biomedical Applications

“…Zhao and coworkers have evaluated the hemostatic efficacy of a biological self-assembling peptide RADA16-I in a rat kidney injury model [44]. Adult male rats were randomized into five groups:…”

Dynamic Research of a Potential Carrier for Hydrophobic Compound Model Pyrene Using Amphiphilic Peptide EYK

“…The discovery of the self-assembly peptide, emerged as a promising nanomaterials, has chart a new direction for us. This category nanomaterial has been widely used in the area of nanoscience and biomedical engineering such as cell and tissue cultures, [1][2][3][4] biological surface engineering, [5][6] membrane protein stabilizations. 7 In recent years, these peptides have also been attracted great interest from scientists used for drug delivery system.…”