Hemorheological changes during experimental acute pancreatitis in male and female rats

Kotán, Róbert; Németh, Norbert; Kiss, Ferenc; Pósán, János; Miszti-Blasius, Kornél; Tóth, L; Furka, I.; Miko, Iren; Sápy, Péter; Szentkereszty, Zsolt

doi:10.1016/j.pan.2012.11.038

Cited by 1 publication

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the 'effective' dosage of cerulein is controversial [4,12,20,43]. Based on the results of our previous study [20] we used 10 g/kg single-dose cerulein subcutaneously in rats, and had the samples 2 hours afterwards. By this protocol we could investigate the early phase of the acute pancreatitis [23,32].…”

Section: Discussionmentioning

confidence: 99%

“…The presence of acute pancreatitis was confirmed by the rise in amylase enzyme activity (1 and 2 hours after cerulein administration), as well as by histomorphological evidences. The largest magnitude of changes was observed in red blood cell deformability and red blood cell aggregation in the group with 10 g/kg cerulein, mostly in female animals [20].…”

Section: Introductionmentioning

confidence: 94%

“…Although the role of microcirculation disturbances in the pathogenesis of acute pancreatitis is well known [5,13,16,18,23,32,41,44], there are only limited number of studies dealing with general hemorheological or micro-rheological changes [e.g., 4,6,7,20]. In the development of microcirculatory disturbance in severe acute pancreatitis numbers of cytokines, inflammatory mediators and reactive oxygen species play role [4,8,12,14,21,23,26,32,34,36,37,42,43].…”

Section: Introductionmentioning

confidence: 99%

“…In the development of microcirculatory disturbance in severe acute pancreatitis numbers of cytokines, inflammatory mediators and reactive oxygen species play role [4,8,12,14,21,23,26,32,34,36,37,42,43]. Microcirculatory disturbances of the pancreas and other organs may alter, or in turn, can be caused by impaired hemorheological parameters [2,4,6,20,27,30,34,42]. However, it is also obvious that influencing the pancreatic microcirculation can be an effective option in the treatment of acute pancreatitis [11-13, 24, 25, 39].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, we have investigated the cerulein-induced acute pancreatitis and provided data on its micro-rheological impact in the rat [20]. It was demonstrated that single dose of 5 or 10 g/kg cerulein-induced acute pancreatitis in rats.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effects of various drugs (flunixin, pentoxifylline, enoxaparin) modulating micro-rheological changes in cerulein-induced acute pancreatitis in the rat

Szentkereszty

Kotán

Kiss

et al. 2014

Clinical Hemorheology and Microcirculation

Self Cite

View full text Add to dashboard Cite

Previously we have investigated the cerulein-induced acute pancreatitis and provided data on its micro-rheological impact in the rat. We hypothesized that non-steroid anti-inflammatory agent flunixin, the xanthine-derivate pentoxifylline and the low molecular weight heparin enoxaparin may have various beneficial effects improving microcirculatory and rheological parameters. In female rats, under general anesthesia, 10 g/kg cerulein s.c. was administered and 2 hours afterwards microcirculation was tested by laser Doppler flowmetry on the tongue and after performing laparotomy on the small intestine, liver and pancreas prior to terminal blood sampling. From blood samples hematological parameters, blood pH, lactate concentration, erythrocyte deformability, osmoscan parameters and erythrocyte aggregation were tested. Compared to normal control in acute pancreatitis group we found severe deterioration in tissue microcirculation together with impaired erythrocyte deformability and enhanced aggregation, accompanied by acidic pH and increasing lactate concentration. Improvement was found when using flunixin (s.c.), pentoxifylline (i.p.) or enoxaparin (s.c.). These drugs could partly improve the blood flux on the surface of the investigated organs, and the flunixin had the most expressed improving effects on micro-rheological parameters. Surprisingly, the improving effect of pentoxifylline on micro-rheological parameters was not obvious (red blood cell deformability did not improved better than in the other treated groups), however, microcirculatory parameters improved.

show abstract