Hemorheological changes and hematometric erythrocyte characteristics in rats after sodium nitrite intoxication

Ivanov, Ivan; Gluhcheva, Yordanka; Petrova, Emilia; Antonova, Nadia

doi:10.1007/s13367-014-0025-1

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…All groups were acclimatized for 7 days prior to treatment. NaNO 2 was administered 50 mg/kg body weight (dissolved in 1 mL distilled water), which refers to rate of LD 50 on rats [20]. NaNO 2 as much as 1 mL/day was given intraperitoneally to the rats for 10 days for treated animals (groups II, III, IV, V, and VI).…”

Section: Methodsmentioning

confidence: 99%

Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

Suparmi

Fasitasari

Martosupono

et al. 2016

Journal of Toxicology

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Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll from Sauropus androgynus (L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2 were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll from S. androgynus and Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2 exposure resulted in significant reductions (p < 0.05) in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly (p > 0.05) to normal values. Chlorophyll from S. androgynus and Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.

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Section: Methodsmentioning

confidence: 99%

Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

Suparmi

Fasitasari

Martosupono

et al. 2016

Journal of Toxicology

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“…Літературні дані свідчать, що за гемічної гіпоксії, спричиненої введеним натрію нітритом (Yrhashev, 2009;Jensen, 2010;Buehler et al, 2011), у тканинах тварин простежується нагромадження рівня активних форм кисню та інтенсифікація процесів ПОЛ. Нітритна інтоксикація супроводжується вираженим мембранотоксичним ефектом (El-Sheikh and Khalil, 2011; Ivanov et al, 2014;Baek et al, 2015). У реальному житті нерідко зустрічається комбінована дія декількох токсичних чинників на організм.…”

Section: вступunclassified

Molecular mechanisms of metabolic disorders in the organs of rats of different ages affected by sodium nitrite in the context of tobacco intoxication

Lyhatskyi

Фіра

Fira

et al. 2017

Regul. Mech. Biosyst.

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Among pollutants a significant threat is posed by heavy metals, nitrate fertilizers and industrial waste. However, people also contribute to this threat through bad habits such as smoking and alcohol. All these factors affect health and can cause death. The aim of this study was to investigate the content of reactive oxygen species, markers of cytolysis of hepatocytes, cardoza and the degree of endogenous intoxication in the organism of rats affected by nitrite of sodium on the background of tobacco intoxication. The experiments were carried out on white rats, which were divided into three age categories: immature, mature and old, who for 30 days were exposed to tobacco smoke. The research animals were divided into three groups. One of them for 24 hours before the end of the experiment was given sodium nitrite, the second group was given sodium nitrite 72 hours before the end of the experiment. 30 days after poisoning we tested the contents of reactive oxygen species in neutrophils, cytolysis of erythrocyte membrane was investigated by erythrocytic index of intoxication, hepatocytes and cardiocytes – aminotransferase activity. The degree of endogenous intoxication was evaluated by the content of average weight molecules in blood serum. Poisoning of rats with sodium nitrite on the background of the 30 day intoxication by tobacco smoke led to a significant formation of ROS in neutrophils of rats of different age groups. We investigated the activity of aminotransferases in the blood serum and the above organs of rats after exposing them to sodium nitrite and 30 day tobacco smoke. In the blood serum of adult and aged rats, the activity of alanine aminotransferase increased in these conditions by 2.1 and 1.6 times, respectively. Sodium nitrite, which was an additional factor in the intoxication of the rats of all age groups by smoke, caused more pronounced changes in the activity of the studied enzyme in the blood serum. In the liver and myocardium of rats of different ages a reliable decrease of this index was observed in all experimental groups. There was a significant increase in the serum of rats at different ages of aspartate aminotransferase activity. Aspartate aminotransferase activity in the myocardium of all experimental animals showed a reduction by 1.2–1.3 times after smoke inhalation and 1.5–1.6 times with simultaneous intoxication of the rats by sodium nitrite and tobacco smoke. Sodium nitrite, as an additional toxin raised the penetration capacity of erythrocytes among rats poisoned with smoke.By the end of the experiment, the erythrocyte index of intoxication increased in all age groups: immature rats by 31.7%, mature – by 36.0%, in old rats – by 23.6%. The next step of our research was to investigate the markers of endogenous intoxication – average molecular weight. After the simultaneous intoxication of rats by sodium nitrate and tobacco smoke, at the end of the experiment the content of average weight molecules was λ = 254 and of average mass λ=280 inthe blood serum of rats of all age groups increased by 3.1–3.3 times compared with the intact control group. Immature rats proved most sensitive to the action of sodium nitrite and tobacco.

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Hemorheological changes and hematometric erythrocyte characteristics in rats after sodium nitrite intoxication

Cited by 2 publications

References 10 publications

Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

Molecular mechanisms of metabolic disorders in the organs of rats of different ages affected by sodium nitrite in the context of tobacco intoxication

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