Hemopoietic progenitor cell identification in fetal and adult blood

Müller, Aixa; Sóyano, Andrés; Guevara, Freddy; Martínez, Bahilda; Soyano, Alberto; Ponte, Benita

doi:10.1590/s1516-84842008000800006

Cited by 1 publication

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“…Using specific monoclonal antibodies which recognize the membrane markers of CD34, these cells can be identified using the immunocytochemical methods (Müller et al,2008; Schumm et al,1999). Mitchell et al (1997) showed that it is possible to isolate these cells phenotypically marked with CD34 membrane antigen of UCB.…”

Section: Introductionmentioning

confidence: 99%

Morphological characterization of the progenitor blood cells in canine and feline umbilical cord

Brolio

Vidane

Zomer

et al. 2011

Microscopy Res & Technique

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The umbilical cord blood (UCB) is an important source of hematopoietic stem cells with great deal of interest in regenerative medicine. The UCB cells have been extensively studied as an alternative to the bone marrow transplants. The challenge is to define specific methods to purify and characterize these cells in different animal species. This study is aimed at morphological characterization of progenitor cells derived from UCB highlighting relevant differences with peripheral blood of adult in dog and cats. Therefore, blood was collected from 18 dogs and 5 cats' umbilical cords from fetus in various developmental stages. The mononuclear cells were separated using the gradient of density Histopaque-1077. Characterization of CD34+ cells was performed by flow cytometric analysis and transmission electron microscopy. Granulocytes (ancestry of the basophiles, eosinophiles, and neutrophiles) and agranulocytes (represented by immature lymphocytes) were identified. We showed for the first time the ultrastructural features of cat UCB cells.

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Section: Introductionmentioning

confidence: 99%