Hemolytic suppression roles of taurine in yellowtail Seriola quinqueradiata fed non-fishmeal diet based on soybean protein

Abstract

To elucidate the hemolytic suppression roles of taurine and the necessity of dietary taurine supplementation in yellowtail Seriola quinqueradiata fed a diet without fishmeal, juvenile fish with an initial body weight of 250 g were fed for 40 weeks in floating net cages on soybean protein diets supplemented with 0, 3.0, 4.5 and 6.0% taurine. Taurine concentration of the experimental diets were 0.03, 33.9, 52.8 and 71.6 mg/g, respectively. On the 21st week, fish fed the taurine unsupplemented diet had inferior growth and feed performances, higher death, and there were incidences of green liver and hemolytic anemia. In this group, hepatic and plasma taurine concentrations, serum osmolality and osmotic tolerance of erythrocytes (EC50 value) were significantly lower, and plasma hydroperoxide concentration was markedly higher than in the taurine supplemented groups. These conditions markedly improved corresponding with the increase of dietary taurine concentration. These results indicate that taurine plays a role in hemolytic suppression through osmoregulation and biomembrane stabilization in fish. In addition, it is suggested that yellowtail requires dietary taurine as an essential nutrition for maintaining physiological condition normally.

Untitled

“…Similar results have been previously reported, whereby the inclusion of taurine to all plant protein diets improve the growth performance in other carnivorous marine organisms, because they are either unable or poorly able to synthesize enough taurine to fulfill their nutritional needs . Therefore, taurine supplementation is necessary in diets that are either partially or totally substituted with plant protein (Lunger, McLean, Gaylord, Kuhn, & Craig, 2007;Goto, Tiba, Sakurada, & Takagi, 2001;Kim et al, 2005;Takagi et al, 2006). Studies in mangrove red snapper (L. argentimaculatus) and spotted rose snapper (L. guttatus) have shown that FM protein can be replaced by up to 24% and 20% with soybean meal protein without taurine supplementation, respectively (Benetti et al, 2002;Silva-Carrillo, Hernández, Hardy, González-Rodríguez, & CastilloVargasmachuca, 2012).…”

Necessity of dietary taurine supplementation for preventing green liver symptom and improving growth performance in yearling red sea bream Pagrus major fed nonfishmeal diets based on soy protein concentrate

“…We have examined the effect of FM replacement on yellowtail Seriola quinqueradiata [11][12][13] and red sea bream Pagrus major [7,14], which are important mariculture species in Japan. In yellowtail, fish fed a non-FM diet based on soy protein concentrate (SPC) exhibited inferior growth and feed utilization together with high incidence of green liver and anemia [11][12][13].…”

“…We have examined the effect of FM replacement on yellowtail Seriola quinqueradiata [11][12][13] and red sea bream Pagrus major [7,14], which are important mariculture species in Japan. In yellowtail, fish fed a non-FM diet based on soy protein concentrate (SPC) exhibited inferior growth and feed utilization together with high incidence of green liver and anemia [11][12][13]. In addition, inferior performance and physiological abnormality of yellowtail fed the non-FM diet based on SPC are markedly improved with dietary taurine supplementation [11][12][13], and the optimal level of taurine supplementation to the SPC diets was estimated as 4.5% [12,13].…”

Suplementação de taurina em dietas com duas concentrações proteicas para pós-larvas de camarão-branco-do-pacífico

“…Os mecanismos pelos quais os efeitos benéficos da taurina no metabolismo animal são alcançados ainda não estão claros, tendo em vista as numerosas e importantes funções fisiológicas nas quais esse composto está envolvido (Huxtable, 1992;Cañas, 2002;Takagi et al, 2006). Por suas características químicas (contém nitrogênio, baixo peso molecular, propriedade acidobásica, estabilidade no tratamento com calor e solubilidade em água), esse aminoácido também pode atuar como estimulador alimentar em peixes e crustáceos (Carr, 1982;Coman et al, 1996).…”