Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles

Abstract

In this study, we aimed to develop a technique for colloidal silver nanoparticle (AgNP) modification in order to increase their stability in aqueous suspensions. For this purpose, 40-nm spherical AgNPs were modified by the addition of either human albumin or Tween-80 (Polysorbate-80). After detailed characterization of their physicochemical properties, the hemolytic activity of the nonmodified and modified AgNPs was investigated, as well as their cytotoxicity and antimicrobial effects. Both albumin- and Tween-80-coated AgNPs demonstrated excellent stability in 0.9% sodium chloride solution (>12 months) compared to nonmodified AgNPs, characterized by their rapid precipitation. Hemolytic activity of nonmodified and albumin-coated AgNPs was found to be minimal, while Tween-80-modified AgNPs produced significant hemolysis after 1, 2, and 24 h of incubation. In addition, both native and Tween-80-covered AgNPs showed dose-dependent cytotoxic effects on human adipose-tissue-derived mesenchymal stem cells. The albumin-coated AgNPs showed minimal cytotoxicity. The antimicrobial effects of native and albumin-coated AgNPs against S. aureus, K. pneumonia, P. aeruginosa, Corynebacterium spp., and Acinetobacter spp. were statistically significant. We conclude that albumin coating of AgNPs significantly contributes to improve stability, reduce cytotoxicity, and confers potent antimicrobial action.

Silver Is Not Equal to Silver: Synthesis and Evaluation of Silver Nanoparticles with Low Biological Activity, and Their Incorporation into C12Alanine-Based Hydrogel

“…The results show that the AgNPs, even at high concentration, have little hemolytic activity. Korolev et al [ 51 ] and Zharkova and team [ 52 ] obtained similar results. They found that the hemolytic activity of unmodified AgNPs was minimal and did not exceed a few percent.…”

Bell Shape Curves of Hemolysis Induced by Silver Nanoparticles: Review and Experimental Assay

“…First, we briefly explain the reasons why it was impossible to include the results of the four publications in Table 1 . These reasons are: the concentration of AgNPs for which hemolysis was measured is not indicated [ 74 ], hemolysis data are presented in mg/dL without indication of initial erythrocyte concentration [ 75 ], data of AgNPs are presented in µM (it is impossible to convert it to µg/mL due to the fact that AgNPs are not molecules) [ 76 ], and hemolysis data presented in the description and in corresponding figures are inconsistent (with a ten-fold difference) [ 77 ].…”

Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM

“…Besides, proteins such as CX3CR1, CSF-1R, CX3CL1 and CSF-1 in Fe 3 O 4 -siPD-L1@M -BV2 were detected by western blot [ 37 ]. The hemolysis of Fe 3 O 4 -siPD-L1@M -BV2 was investigated by using rat erythrocytes [ 43 ]. The release of siPD-L1 from Fe 3 O 4 -siPD-L1@M -BV2 in GSH-containing solution was observed by gel retardation assay [ 44 ].…”