Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Silver Nanoparticles Conjugated with Lincomycin or Cefazolin

Abstract

The overuse of antibiotics has led to the emergence of resistant bacteria. A good alternative is silver nanoparticles, which have antibacterial activity against Gram-negative and Gram-positive bacteria, including multidrug-resistant strains. Their combination with already known antibiotics has a synergistic effect. In this work, we studied the synthesis of conjugates of silver nanoparticles with two antibiotics, lincomycin and cefazolin. Albumin and glutathione were used as spacer shells with functional groups. The physicochemical properties of the obtained conjugates, their cytotoxicity and synergism of antimicrobial activity were studied. The 50% antimicrobial activity of the obtained samples was shown, which allows them to be recommended for use as topical drug preparations.

Effects of Sulforaphene on the Cariogenic Properties of Streptococcus Mutans In Vitro and Dental Caries Development In Vivo

“…The hemolytic activity of SFE was further investigated. The off-fiber sheep blood (1001339-1, Hopebio) was centrifuged at 2000× g for 5 min to collect the red cells [ 45 ]. The red cells were incubated in PBS (1 mL) containing SFE at 37 °C for 30 min.…”

Novel Silver Nanocomposites as Effective Antitumor Agents

“…Few reviews and articles have demonstrated silver nanocomposites to be able to inhibit human cancer cells via different mechanisms. [9][10][11][12][13][14][15][16][17] In ref. [9] the cytotoxic activity of silver nanoparticles (<28 nm) and silver ions against T47D breast cancer cells was studied.…”

Synergistic Antifungal Interaction between Pseudomonas aeruginosa LV Strain Metabolites and Biogenic Silver Nanoparticles against Candida auris

“…Combined therapy approaches to control infections have been another attractive strategy, especially in those caused by multidrug-resistant microorganisms [34,35]. Some advantages may be associated with the use of combined therapy, such as the synergistic effect due to action on multiple targets, a broad spectrum of action, improvement in the bioavailability of one or more compounds, and reduced risk of both the toxicity and the emergence of resistance during treatment [36].…”

“…This antifungal combination aims to reduce the fungal burden on the host and the risk of treatment failure [37]. From this perspective, the combination of silver nanopar-ticles (AgNP) with different compounds, including antimicrobials, has been extensively investigated [34,35].…”