Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR

Esmaeel, Jameela Radi; Sadeq, Jenan Nadhim

doi:10.29079/vol17iss2art504

QJVMS

2018

DOI: 10.29079/vol17iss2art504

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Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR

Jameela Radi Esmaeel

Jenan Nadhim Sadeq

Abstract: Hemolysin protein is exotoxin produce by organisms that cause lysis of blood cells. This study was conducted to screen the presence of hemolysin gene from 20 isolates of Klebsiella pneumonia based 16S rRNA genes by using specific primer. This gene potent the pathogenesis of Klebsiella pneumonia. The primer was designed in this study by NCBI-GenBank and primer3 plus. (Bioneer Company provided the primers. Korea). Molecular detection of isolates, which give away specific PCR products of 505bp for hly gene, hemol… Show more

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Cited by 2 publications

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Phenotypic Characterization of Virulence Factors and Antibiogram of Klebsiella pneumoniae Isolates from Various Clinical Samples – A Cross Sectional Study

Hullur¹,

Natarajan²,

Sreeramulu³

2022

J. Pure Appl. Microbiol.

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K. pneumoniae is known to cause hospital and community acquired infections. It is usually associated with upper & lower respiratory infections, septicaemia, urinary tract infection, wound infections, neonatal sepsis, meningitis, and endophthalmitis. The virulence factors play a role in its existence in different environmental conditions and therefore help in establishing Klebsiella pneumoniae infection in the human body. Multi drug resistant Klebsiella pneumoniae is an increasing threat to human health. Klebsiella pneumoniae is one of the species recognized as nosocomial pathogens that exhibit multidrug resistance and virulence in ESKAPE group as per WHO. The study was conducted to determine the various virulence factors & the antimicrobial pattern of Klebsiella pneumoniae isolates. A cross sectional observational study, conducted in Department of Microbiology of R.L. Jalappa Hospital and Research Centre, Kolar, Sample size of 150. All 150 Klebsiella pneumoniae isolates collected for the study, The Klebsiella pneumoniae isolates which were positive for various virulence factors were as follows on hemolysis 7(4.66%), capsule 150(100%), Hypermucoviscosity formation 66(44%), biofilm production 81(54%), siderophore production 110(73.33%), protease 135(90%), gelatinase 126(84%), lipase production 119(79.33%), lecithinase activity 82(54.66%). The drug resistance klebsiella pneumoniae were as follows: ESBL producers 24(16.67%), AmpC producers were 22(14.67%), MDR 116(74.20%), extensive drug resistant (XDR) 30(20%), pan drug resistant (PDR) 42(28%), Carbapenem resistance 65.33% reported. The increasing coexistence of virulence factors & antimicrobial resistance pattern is of particular concern. Hence active surveillance for antimicrobial resistance & virulence determinants is imperative now to implement effective control measures to prevent the rapid spread of drug resistance.

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Phenotypic Characterization of Virulence Factors and Antibiogram of Klebsiella pneumoniae Isolates from Various Clinical Samples – A Cross Sectional Study

Hullur¹,

Natarajan²,

Sreeramulu³

2022

J. Pure Appl. Microbiol.

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Revisiting ESKAPE Pathogens: virulence, resistance, and combating strategies focusing on quorum sensing

Venkateswaran,

Vasudevan,

David

et al. 2023

Front. Cell. Infect. Microbiol.

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The human–bacterial association is long-known and well-established in terms of both augmentations of human health and attenuation. However, the growing incidents of nosocomial infections caused by the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp.) call for a much deeper understanding of these organisms. Adopting a holistic approach that includes the science of infection and the recent advancements in preventing and treating infections is imperative in designing novel intervention strategies against ESKAPE pathogens. In this regard, this review captures the ingenious strategies commissioned by these master players, which are teamed up against the defenses of the human team, that are equally, if not more, versatile and potent through an analogy. We have taken a basketball match as our analogy, dividing the human and bacterial species into two teams playing with the ball of health. Through this analogy, we make the concept of infectious biology more accessible.

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Hemolysin gene detection in some isolates of Klebsiella pneumonia by PCR

Cited by 2 publications

References 9 publications

Phenotypic Characterization of Virulence Factors and Antibiogram of Klebsiella pneumoniae Isolates from Various Clinical Samples – A Cross Sectional Study

Phenotypic Characterization of Virulence Factors and Antibiogram of Klebsiella pneumoniae Isolates from Various Clinical Samples – A Cross Sectional Study

Revisiting ESKAPE Pathogens: virulence, resistance, and combating strategies focusing on quorum sensing

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