Hemograma e proteínas de fase aguda de bezerros sadios do nascimento aos 30 dias de idade

Rocha, Thaís Gomes; Nociti, Ricardo Perecin; Sampaio, Alexandre Amstalden Moraes; Fagliari, José Jurandir

doi:10.1590/s0100-736x2013001300005

Cited by 21 publications

(26 citation statements)

References 20 publications

(29 reference statements)

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“…These differences can be associated with stress (Murata et al 2004), ingestion or not of colostrum (Orro et al 2008) and birth from multiparous or primiparous cows. Calves from primiparous cows generally present lower serum concentration of α1-GPA than those from multiparous cows (Rocha et al 2013). There are no reports of α1-GPA levels in the CSF of cattle, but α1-GPA is considered a sensitive marker for bacterial meningitis in pigs and rabbits (Itoh et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

et al. 2018

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Use of acute-phase proteins (APPs) for assessment of health and disease in animals has increased greatly within the last decade. The objective was to determine the normal concentration of APPs in the serum and cerebrospinal fluid (CSF) of healthy cattle by polyacrylamide gel electrophoresis. Fifty crossbred animals (350±70kg of BW and 18±1.2 months of age), 25 heifers and 25 steers were used. CSF samples were collected from atlanto-occipital (AO) site and blood samples were obtained from the jugular vein. CSF and serum protein electrophoresis were performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-seven proteins with molecular weights ranging from 7 and 37kDa were identified in CSF of all animals. These eight were nominally identified with immunoglobulin A and G, celuloplasmin, transferrin, albumin, α1-antitripsin, acidic glycoprotein, and haptoglobin. All protein fractions in CSF did not differ between heifers and steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, but only the CSF presented α1-antitripsin. The serum values of acidic glycoprotein and immunoglobulin G were significantly higher in steers compared with heifers. In conclusion, measurement of CSF acute phase protein concentrations can be useful in diagnosing and monitoring the progression of bovine neurological diseases, perhaps even to guide therapeutic procedures. The CSF electrophoretic profile of healthy cattle does not change depending on gender.

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Section: Discussionmentioning

confidence: 99%

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

et al. 2018

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“…No entanto, para a raça Crioula Lageana variedade mocha os valores desta variável apresentaram diminuição seguido por aumento neste período. Concordando com observações de outros autores em outras raças, os valores de CHCM apresentaram pequenas oscilações durante o primeiro mês de vida [3,4,24] (Tabela 1).…”

Section: Discussionunclassified

Hemogram, Fibrinogen Concentration and Plasma Total Protein from Calves of Criollo Lageano Breed Variety Hornless and Aberdeen Angus Breed (Red Angus) on the First Six Months of Life

Schade

Rossi

Saito

et al. 2016

Acta Scientiae. Vet.

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Background: Neonatal period in bovine presents important variations in hematologic and immunologic parameters, and the resulting mortality rates may be due to increased susceptibility to disease. Race, age, environmental, climate and management factors are sources of CBC's parameters variation in cattle. The objective of this study is to evaluate and compare the CBC variables and concentrations of total protein (PTP) and plasma fibrinogen calves of Criolo Lageano Breed variety hornless and Aberdeen Angus (Red Angus) in the first six months of life, which are produced under extensive management at the Planalto Serrano Catarinense (southern Brazil). Materials, Methods & Results:Two groups were selected, comprising 11 calves of Hornless Criolo Lageano and 14 calves of the Aberdeen Angus breed (Red Angus). Blood samples were collected for complete blood count and determination of total protein concentrations, and plasma fibrinogen in the periods between 24 h and 36, 15, 30, 60, 90, 120, 150 and 180 days of life. The statistical analysis was performed using analysis of variance for repeated measures (ANOVA) and Tukey's test (P < 0.05). During the experimental period infestations by ticks in both races were observed. In the first month of life, blood smears revealed the presence of blood parasites of the genus Babesia spp. and Anaplasma sp. The fluctuations seen for erythrocyte in the neonatal period (30 days), although not significant, consisted of raising the number of values of erythrocytes, hemoglobin (Hb) and packed cell volume (PCV), and reduction in mean corpuscular volume values (MCV). From 90 days of age there was a significant reduction in number of red blood cells and VG values, and elevated MCV values and mean corpuscular volume (MCHC) for the Aberdeen Angus (Red Angus). For the WBC, the neonatal period was marked by increase in the number of lymphocytes and decrease in neutrophil ratio: lymphocyte (N, L) with respect to the inversion of 15 days. In the age group 90 days of life was evident increase in the number of leukocytes and lymphocytes in both races. The plasma fibrinogen concentrations showed no significant variations during the trial period. The plasma total protein (PTP), had higher values on the second day of life, with reductions up to 60 days and gradual increase in subsequent times. Discussion: The fluctuations related to the values of erythrocyte variables in the neonatal period are consistent with results reported by other authors in calves of other races. Changes in erythrocyte values observed from 90 days of age on race Aberdeen Angus (Red Angus) can be attributed to infection by blood parasites and consequent hemolysis. As for the leucocyte count, increasing the number of lymphocytes and inversion of N:L is the neonatal period in a physiological variation and confirms observations made by other authors in calves of other races. In the age group 90 days, leukocytosis by lymphocytosis was related to natural premonition against Babesia spp. and Anaplasma spp., which is documented in cattle i...

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“…Estudos realizados em bezerros por outros autores mostraram que os valores mais baixos de transferrina são encontrados ao nascimento e tendem a se elevar após a ingestão do colostro; entretanto, os mesmos achados não foram observados neste trabalho (Rocha et al, 2013;Santos, et al, 2013). É possível que, na espécie caprina, essa dinâmica possa ser diferente ou sofrer influência de outros fatores.…”

Section: Methodsunclassified

Aquisição de imunidade passiva em cabritos alimentados com colostro de cabras com e sem mastite

Alcindo¹,

Braga²,

Poló³

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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O objetivo deste trabalho foi avaliar a transferência de imunidade passiva de cabras, que pariram com mastite, para seus respectivos cabritos. Os animais foram distribuídos em dois grupos, a saber: grupo 1 (GI), constituído por cabritos, filhos de cabras sem isolamento microbiológico em ambas as glândulas mamárias, e grupo 2 (GII), composto por cabritos, filhos de cabras com resultado positivo à lactocultura, em pelo menos uma das glândulas mamárias. Foram coletadas amostras de colostro e sangue à parição, bem como às 24 e às 48 horas após o parto/nascimento. O diagnóstico e o monitoramento da mastite nos animais foram realizados por meio do California Mastitis Test (CMT), contagem de células somáticas e isolamento microbiológico. A proteína total foi mensurada pelo método do biureto, e as concentrações de imunoglobulina A (IgA), imunoglobulina G (IgG), transferrina, albumina e haptoglobina por meio da eletrofoerese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE). Os agentes mais isolados na cultura microbiológica foram os Staphylococcus coagulase negativa. Não houve diferença significativa (P<0,05) entre os valores médios de imunoglobulina G (IgG) nos cabritos provenientes de cabras com mastite quando comparados aos recém-nascidos oriundos de cabras livres de infecções intramamárias. Da mesma forma, a atividade de gamaglutamiltransferase (GGT) não mostrou diferença entre os grupos em todos os momentos avaliados. A ingestão de colostro decorrente de cabras com mastite não causou falha na transferência de imunidade passiva nos respectivos conceptos.

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Hemograma e proteínas de fase aguda de bezerros sadios do nascimento aos 30 dias de idade

Cited by 21 publications

References 20 publications

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

Hemogram, Fibrinogen Concentration and Plasma Total Protein from Calves of Criollo Lageano Breed Variety Hornless and Aberdeen Angus Breed (Red Angus) on the First Six Months of Life

Aquisição de imunidade passiva em cabritos alimentados com colostro de cabras com e sem mastite

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