Hemoglobinolytic Activity of Serum in Mice Infected with Schistosoma Mansoni *

Diagnostic Mr31 000Schistosoma mansoniproteins: requirement of infection, but not immunization, and use of the “miniblot” technique for the production of monoclonal antibodies

“…During infection, however, the M r 31 000 protein could conceivably stimulate an immune response, if it were continuously released into the host's circulation as an excretory/secretory antigen. Interestingly, the haemoglobinolytic enzyme in the schistosome gut (TIMMS & BUEDING, 1959) has a molecular weight between M r 27 000 (SAUER & SENFT, 1972) and 32 000 (DEELDER etal, 1977) and is present in the serum of infected mice (SENFT et al, 1981). Moreover, schistosome regurgitant contains proteinase activity in the molecular mass range between 28 800 and 31 600 dalton (CHAPPELL & DRESDEN, 1986).…”

Complex formation of human alpha‐1–antitrypsin with components in Schistosoma mansoni cercariae

“…Proteolytic enzymes play key roles in the survival of the parasitic trematode Schistosoma mansoni (McKerrow & Doenhoff 1988). These include digestion of host skin to facilitate penetration (Landsperger, Stirewalt & Dresden 1982, McKerrow et al 1983, Newport et al 1988, Marikovsky, Arnon & Fishelson 1990, degradation of haemoglobin to aid parasite nutrition (Lindquist et al 1986, Senft, Goldberg & Byram 1981, Chappell & Dresden 1987, destruction of antibody for immune evasion (Auriault et al 1980(Auriault et al , 1981 and digestion of host tissues from excretion of parasite eggs (Pino-Heiss, Brown & McKerrow 1985, Sung & Dresden 1986). Of equal importance in the host is the role of a major group of blood proteins, the plasma protease inhibitors, in maintaining homeostasis, by inactivating and removing endogenous (stray, excess or redundant) and exogenous proteolytic activity (Laskowski & Kato 1980, Travis & Salvesen 1983.…”

Recent advances in schistosome biochemistry

“…L-phenylalanine inhibited poteases in Senft's studies but not in those of Deelder, Reinders & Rotmans (1977), suggesting the enzyme could vary between strains of worms. As the worms feed, the protease is regurgitated into the host blood and haemoglobinolytic activity found in serum of infected mice is very probably from the schistosomes (Senft et al 1981). The enzyme can therefore be expected to sensitize the host, and purified enzyme injected into infected animals induces a histaminic skin response which is specific for the schistosome species (Senft & Maddison, 1975;Senft, Weltman, Goldgraber, Egyud & Kuntz, 1979).…”