Hemoglobin in Arthropods—Daphnia as a Model

Zeis, Bettina

doi:10.1007/978-3-030-41769-7_6

Cited by 9 publications

(13 citation statements)

References 93 publications

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“…Haemoglobin (henceforward "Hb") production was observed to increase in response to food levels in D. magna (Fox et al, 1951;Zeis, 2020). However, no further studies have considered the relationship between nutrition and Hb in Daphnia.…”

Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

“…Daphnia magna encodes 12 di-domain Hb genes [as annotated in the gene set: (Orsini et al, 2016)] that result from a complex series of duplications (Colbourne et al, 2011;Zeis, 2020). Sixteen Hb di-domains are aggregated to form the free-circulating D. magna Hb molecule (Zeis, 2020). Different combinations of Hb isoforms produce structurally distinct Hb molecules, which in turn have different oxygen-binding characteristics (Zeis, 2020).…”

Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

“…Sixteen Hb di-domains are aggregated to form the free-circulating D. magna Hb molecule (Zeis, 2020). Different combinations of Hb isoforms produce structurally distinct Hb molecules, which in turn have different oxygen-binding characteristics (Zeis, 2020). The induction of Hb genes in Daphnia is mediated by the transcription factor hypoxia-inducible factor (HIF) (Gorr, Cahn, Yamagata, & Bunn, 2004;Zeis, 2020), a heterodimer formed from HIF-1α and HIF-1β proteins.…”

Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

“…Different combinations of Hb isoforms produce structurally distinct Hb molecules, which in turn have different oxygen-binding characteristics (Zeis, 2020). The induction of Hb genes in Daphnia is mediated by the transcription factor hypoxia-inducible factor (HIF) (Gorr, Cahn, Yamagata, & Bunn, 2004;Zeis, 2020), a heterodimer formed from HIF-1α and HIF-1β proteins. The HIF-1 complex is an important factor in extending longevity of Caenorhabditis elegans under CR (Di Chen & Kapahi, 2009;Lee, Hwang, & Kenyon, 2010;Y.…”

Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

“…This is evidenced by a change in the proportions of expressed isoforms of gene Dapma7bEVm014981 under CR in favour of isoforms without (transcript 21) an erythrocruorin domain or two domains only (transcript 11). Thus, variation in erythrocruorin domains could underlie the observed correlation between oxygen-binding capacity and structurally distinct Hb gene isoforms (Zeis, 2020).…”

Section: A Haemoglobin Response To Cr In D Magnamentioning

confidence: 99%

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Daphnia magna modifies its gene expression extensively in response to caloric restriction revealing a novel effect on haemoglobin isoform preference

et al. 2020

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Caloric restriction (CR) produces clear phenotypic effects within and between generations of the model crustacean Daphnia magna. We have previously established that micro‐RNAs and cytosine methylation change in response to CR in this organism, and we demonstrate here that CR has a dramatic effect on gene expression. Over 6,000 genes were differentially expressed between CR and well‐fed D. magna, with a bias towards up‐regulation of genes under caloric restriction. We identified a highly expressed haemoglobin gene that responds to CR by changing isoform proportions. Specifically, a transcript containing three haem‐binding erythrocruorin domains was strongly down‐regulated under CR in favour of transcripts containing fewer or no such domains. This change in the haemoglobin mix is similar to the response to hypoxia in Daphnia, which is mediated through the transcription factor hypoxia‐inducible factor 1, and ultimately the mTOR signalling pathway. This is the first report of a role for haemoglobin in the response to CR. We also observed high absolute expression of superoxide dismutase (SOD) in normally fed individuals, which contrasts with observations of high SOD levels under CR in other taxa. However, key differentially expressed genes, like SOD, were not targeted by differentially expressed micro‐RNAs. Whether the link between haemoglobin and CR occurs in other organisms, or is related to the aquatic lifestyle, remains to be tested. It suggests that one response to CR may be to simply transport less oxygen and lower respiration.

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Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

Section: Daphnia Haemoglobins and The Link To Crmentioning

confidence: 99%

Section: A Haemoglobin Response To Cr In D Magnamentioning

confidence: 99%

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Daphnia magna modifies its gene expression extensively in response to caloric restriction revealing a novel effect on haemoglobin isoform preference

et al. 2020

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The interplay between prior selection, mild intermittent exposure, and acute severe exposure in phenotypic and transcriptional response to hypoxia

Ekwudo

Malek

Anderson

et al. 2022

Ecology and Evolution

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Hypoxia has profound and diverse effects on aerobic organisms, disrupting oxidative phosphorylation and activating several protective pathways. Predictions have been made that exposure to mild intermittent hypoxia may be protective against more severe exposure and may extend lifespan. Here we report the lifespan effects of chronic, mild, intermittent hypoxia, and short‐term survival in acute severe hypoxia in four clones of Daphnia magna originating from either permanent or intermittent habitats. We test the hypothesis that acclimation to chronic mild intermittent hypoxia can extend lifespan through activation of antioxidant and stress‐tolerance pathways and increase survival in acute severe hypoxia through activation of oxygen transport and storage proteins and adjustment to carbohydrate metabolism. Unexpectedly, we show that chronic hypoxia extended the lifespan in the two clones originating from intermittent habitats but had the opposite effect in the two clones from permanent habitats, which also showed lower tolerance to acute hypoxia. Exposure to chronic hypoxia did not protect against acute hypoxia; to the contrary, Daphnia from the chronic hypoxia treatment had lower acute hypoxia tolerance than normoxic controls. Few transcripts changed their abundance in response to the chronic hypoxia treatment in any of the clones. After 12 h of acute hypoxia treatment, the transcriptional response was more pronounced, with numerous protein‐coding genes with functionality in oxygen transport, mitochondrial and respiratory metabolism, and gluconeogenesis, showing upregulation. While clones from intermittent habitats showed somewhat stronger differential expression in response to acute hypoxia than those from permanent habitats, contrary to predictions, there were no significant hypoxia‐by‐habitat of origin or chronic‐by‐acute treatment interactions. GO enrichment analysis revealed a possible hypoxia tolerance role by accelerating the molting cycle and regulating neuron survival through upregulation of cuticular proteins and neurotrophins, respectively.

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The effect of temperature and predation on performance in monoculture and in competition in three Daphniidae differing in body size

Bomfim

Vanvelk

Pinheiro-Silva

et al. 2023

Limnology & Oceanography

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Zooplankton body size shows a strong association with temperature, competition, and predation. Global warming affects all three drivers of body size and is thus expected to lead to substantial changes in zooplankton community composition and body size distributions. To disentangle the isolated and joint effect of temperature, competition, and fish predation on species biomass and community composition in zooplankton, we monitored population biomasses of three Daphniidae species that differ in body size (Daphnia magna, Daphnia pulex, and Ceriodaphnia reticulata) for 20 days, manipulating competition (monoculture, pairwise trials, and three-species communities), temperature (20 C, 24 C, and 28 C) and presence or absence of fish predation. In the absence of predation, D. magna dominated in all competition experiments, even at high temperatures. D. magna went extinct, however, in the predation treatments at 24 C and 28 C. D. pulex outcompeted C. reticulata and was negatively affected by predation and high temperature. C. reticulata did not reduce biomass at high temperatures and was negatively affected by all competition trials, but was positively affected by predation. Our results indicate that the two larger-bodied species are more negatively affected by the combination of temperature and predation than the smallest species. While higher temperatures reduced the biomass of the larger-bodied species, it did not fundamentally change their ability to dominate over the smallest species in competition. The combined effect of warming and predation changed community composition more fundamentally, resulting in the dominance of small-bodied species. This can have important ecosystem-wide impacts, such as the transition to turbid, algae-dominated systems.

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Hemoglobin in Arthropods—Daphnia as a Model

Cited by 9 publications

References 93 publications

Daphnia magna modifies its gene expression extensively in response to caloric restriction revealing a novel effect on haemoglobin isoform preference

Daphnia magna modifies its gene expression extensively in response to caloric restriction revealing a novel effect on haemoglobin isoform preference

The interplay between prior selection, mild intermittent exposure, and acute severe exposure in phenotypic and transcriptional response to hypoxia

The effect of temperature and predation on performance in monoculture and in competition in three Daphniidae differing in body size

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