Hemoglobin deoxygenation and methemoglobinemia prevent regulatory volume decrease in crucian carp (Carassius carassius) red blood cells

Andreyeva, A. Yu.; Солдатов, А. А.; Кривченко, А. И.; Mindukshev, Igor; Gambaryan, Stepan

doi:10.1007/s10695-019-00689-4

Cited by 7 publications

(11 citation statements)

References 37 publications

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“…The physiological osmolarity of the ark clam hemolymph (470–500 mOsm/L) was almost equal to seawater (461 mOsm/L), in contrast to aquatic vertebrates, which plasma osmolarity is substantially lower (220 mOsm/L in the common frog, 230–290 mOsm/L in freshwater teleosts, and 300–350 mOsm/L in marine fish) (Andreyeva et al, 2018, 2019; Tsui et al, 2012). The value of H50 of the ark clam hemocytes was five times lower than the normal osmolarity of hemolymph.…”

Section: Resultsmentioning

confidence: 99%

“…To answer these questions we used laser diffraction method for analysis of volume changes of the ark clam hemocytes following hyposmotic stimulation. This method was successfully used for measuring volume changes in RBCs of lower and higher vertebrates (Andreyeva et al, 2018, 2019; Mindukshev et al, 2007, 2016, 2019; Sudnitsyna et al, 2020). We show that hemoglobin‐containing hemocytes of ark clam react to hyposmotic conditions by activation of RVD response which is independent of Hb conformational state.…”

Section: Introductionmentioning

confidence: 99%

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Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

et al. 2022

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Many bivalve species are considered to be euryhaline organisms due to effective adaptation to fluctuations of environmental salinity. Cellular mechanisms responsible for tolerance to salinity changes remain unclear for bivalves despite this question being critically important for commercially cultured species frequently introduced into regions differing from natural habitat by salinity regime. In the present work laser diffraction method was used for the analysis of volume changes in hemoglobin‐containing ark clam (Anadara kagoshimensis) hemocytes following hyposmotic stimulation. Hemocytes responded to hyposmotic shock (decrease of media osmolarity from 461 to 216 mОsm/L) by a rapid swelling up to 171.5 ± 15.2% of control level. At normal osmotic conditions (osmolarity 461 mOsm/L), hemocyte mean cellular volume (MCV) was 354.0 ± 24.4 fl and maximum MCV of hyposmotically swollen cells prior lysis was 555.5 ± 57.4 fl (at the osmolarity 194 mOsm/L). Ark clam hemocytes demonstrated volume recovery response following hyposmotic swelling. Regulatory volume decrease (RVD) reaction did not depend on hemoglobin confirmation status. Final MCV of swollen hemocytes at the end of experimental period of RVD in oxygenated and deoxygenated suspensions did not significantly differ.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

et al. 2022

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“…5b). It is known that an increase in the proportion of deoxyHb under hypoxia leads to the displacement of glycolysis enzymes from the binding site to CDB3 and their transition to a soluble active state (D'Alessandro et al, 2015;Chu et al, 2016;Andreyeva et al, 2019;Kosmachevskaya et al, 2019), the metabolic flow is directed to the glycolytic pathway, which allows the production of ATP. During this time, the pentose phosphate pathway of glucose metabolism is inhibited and glycolysis is activeted, which reduces the ability of erythrocytes to synthesize NADPH and reduce glutathione, which in turn are required for the reduction of dehydroascorbate to ascorbate as one of the electron transport donors (Tu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…An increase in the proportion of deoxyhemoglobin (deoxyHb) under hypoxia leads to the displacement of glycolysis enzymes from the CDB3 binding site and their transition to a soluble active state (D'Alessandro et al, 2015;Kosmachevskaya et al, 2019). It is believed that the redistribution of hemoglobin (Hb) between soluble and membrane-bound forms switches metabolism from the pentose phosphate pathway to the glycolytic in response to changes in cell oxygen status (D'Alessandro et al, 2015;Sega et al, 2015;Andreyeva et al, 2019). On the one hand, it increases the level of intracellular ATP, and, on the other hand, reduces the glucoses flux that enter the pentose phosphate pathway, which produces NADPH, necessary for the work of antioxidant enzymes, and reduced glutathione.…”

Section: Introductionmentioning

confidence: 99%

Potential role of cytoplasmic protein binding to erythrocyte membrane in counteracting oxidative and metabolic stress

Dotsenko

Mykutska

Taradina

et al. 2020

Regul. Mech. Biosyst.

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The ability of protein to reversibly bind with membrane components is considered to be one of the oldest mechanisms of cell response to external stimuli. Erythrocytes have a well-developed mechanism of an adaptive response involving sorption-desorption processes, e.g. interactions of key glycolytic enzymes and hemoglobin with band 3 protein. A few publications have shown that under oxidative stress, cytoplasmic enzymes such as catalase, glutathione peroxidase and рeroxiredoxin bind to the erythrocyte membrane. The present work is a continuation of research in this direction to determine the causes and consequences of the interaction of cytoplasmic proteins with the membrane under conditions of oxidative stress and different glucose content. Human erythrocytes were incubated for five hours at 20 °C in an oxidizing medium of AscH – 1 · 10–4 M, Cu2+– 5 · 10–6 M with different glucose content (0–8 mM). Dynamic changes in the accumulation of membrane-bound hemoglobin, the distribution of ligand forms of hemoglobin in the cytoplasmic and membrane-bound fractions, the activity of membrane-associated and cytoplasmic forms of Cu/Zn superoxide dismutase (SOD1) and catalase, H2O2 content in extracellular and intracellular media were recorded. It was shown that binding of catalase and SOD1 to the erythrocyte membrane is initiated by oxidative stress and is a physiological function aimed at complete inactivation of extracellular and H2O2 and protection against their entry into the cell. It was shown that under conditions of glucose depletion and oxidative loading, catalase and SOD1 bind to the erythrocyte membrane, leading to inactivation of these enzymes. Membrane-bound hemoglobin was higher in cells incubated under these conditions than in glucose experiments. Glucose introduced into the incubation medium in an amount 4–8 mM causes complete binding of SOD1 to the membrane of erythrocytes, by involving it in the processes of casein kinase stabilization and glycolytic fluxes regulation. With mild oxidation, the amount of hemoglobin bound to the membrane does not change, indicating the presence of certain binding sites for hemoglobin with membrane proteins. We show that the activity of membrane-bound SOD1 along with the content of ligand forms in the composition of membrane-bound hemoglobin are informative indicators of the metabolic and redox state of erythrocytes.

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“…The unilateral ABI calculation is the ratio of the systolic pressure of the lateral ankle artery (posterior tibial artery or dorsal foot artery) to the highest value of the bilateral brachial artery systolic pressure. 4 Take the low value of ABI on both sides for statistical analysis. Pulse pressure (PP) measurement.…”

Section: Determination Of Pwv/abimentioning

confidence: 99%

Improvement of Cardiovascular Function Health Level by Regular Sports

Wang

Zhao

Sun

et al. 2021

Rev Bras Med Esporte

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Introduction Regular physical activity helps improve cardiovascular and cerebrovascular skills. How to evaluate the nervous tension of the cardiovascular and cerebrovascular vessels through sports is a hot topic. Objective The paper discusses the influence of regular participation in sports on people’s cardiovascular function and blood-related indicators. Methods We select 30 healthy older adults who regularly participate in sports, record their ECG changes, blood pressure, heart rate and other related cardiovascular function indicators, and analyze the blood function of the elderly. Detection of blood cell count (RBC), red blood cell volume (MCV) and hemoglobin (Hb), serum creatinine (Cr), blood glucose (BGS), triglycerides (TG), cholesterol (TC), low-density lipoprotein (LDL) and high-Density lipoprotein (HDL) is measured. Results Older adults who persist in exercise for a long time have better indicators than those who do not exercise. Conclusions Appropriate aerobic exercise can reduce the stiffness of blood vessels in the elderly. Exercise can help the elderly increase heart rate variability and improve the heart’s autonomic nerve function’s blood indicators, and body mass. Level of evidence II; Therapeutic studies - investigation of treatment results.

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Hemoglobin deoxygenation and methemoglobinemia prevent regulatory volume decrease in crucian carp (Carassius carassius) red blood cells

Cited by 7 publications

References 37 publications

Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

Cellular osmoregulation of the ark clam (Anadara kagoshimensis) hemocytes to hyposmotic media

Potential role of cytoplasmic protein binding to erythrocyte membrane in counteracting oxidative and metabolic stress

Improvement of Cardiovascular Function Health Level by Regular Sports

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