Hemoglobin Denaturation Caused by Surfactants.

Hayashi, Tetsu; Itagaki, Hiroshi; Fukuda, Toshio; Tamura, Uhei; Satô, Yukio; Suzuki, Yasuo

doi:10.1248/bpb.18.540

Cited by 17 publications

(10 citation statements)

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“…The protein-denaturing property of OPB was estimated with a hemoglobin denaturation assay, as previously described (24,25). Briefly, test antiseptic dilutions prepared with distilled water (12 concentrations from 0 to 20,000 g/ml prepared by the serial 2-fold dilution method) were placed on a 96-well microplate.…”

Section: Methodsmentioning

confidence: 99%

Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic

Hagi

Iwata

Nii

et al. 2015

Antimicrob Agents Chemother

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Olanexidine gluconate [1-(3,4-dichlorobenzyl)-5-octylbiguanide gluconate] (development code OPB-2045G) is a new monobiguanide compound with bactericidal activity. In this study, we assessed its spectrum of bactericidal activity and mechanism of action. The minimal bactericidal concentrations of the compound for 30-, 60-, and 180-s exposures were determined with the microdilution method using a neutralizer against 320 bacterial strains from culture collections and clinical isolates. Based on the results, the estimated bactericidal olanexidine concentrations with 180-s exposures were 869 g/ml for Gram-positive cocci (155 strains), 109 g/ml for Gram-positive bacilli (29 strains), and 434 g/ml for Gram-negative bacteria (136 strains). Olanexidine was active against a wide range of bacteria, especially Gram-positive cocci, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci, and had a spectrum of bactericidal activity comparable to that of commercial antiseptics, such as chlorhexidine and povidone-iodine. In vitro experiments exploring its mechanism of action indicated that olanexidine (i) interacts with the bacterial surface molecules, such as lipopolysaccharide and lipoteichoic acid, (ii) disrupts the cell membranes of liposomes, which are artificial bacterial membrane models, (iii) enhances the membrane permeability of Escherichia coli, (iv) disrupts the membrane integrity of S. aureus, and (v) denatures proteins at relatively high concentrations (>160 g/ ml). These results indicate that olanexidine probably binds to the cell membrane, disrupts membrane integrity, and its bacteriostatic and bactericidal effects are caused by irreversible leakage of intracellular components. At relatively high concentrations, olanexidine aggregates cells by denaturing proteins. This mechanism differs slightly from that of a similar biguanide compound, chlorhexidine.

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Section: Methodsmentioning

confidence: 99%

Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic

Hagi

Iwata

Nii

et al. 2015

Antimicrob Agents Chemother

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“…The method is based on the hemoglobin denaturation induced by irritant products (Hayashi et al, 1995;Vinardell et al, 1999) and was modified using hemoglobin from human erythrocytes (Mitjans et al, 2003). Hemoglobin was exposed to various concentration of the infusion in 96-well plates and its absorbance was recorded.…”

Section: Study Of Potential Eye Irritant Actionmentioning

confidence: 99%

In vitro study of the antioxidant and immunomodulatory activity of aqueous infusion of Bidens pilosa

Abajo¹,

Boffill

Campo³

et al. 2004

Journal of Ethnopharmacology

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“…However, use of surfactant may cause protein denaturation. [16,17] Further, the toxicity issue associated with surfactant molecule may limit its use via a particular route. In the present study, we have employed dextran sulfate (DS), molecular weight 9000–20000 Da as a model polymer to form HIP complex.…”

Section: Introductionmentioning

confidence: 99%

Design and evaluation of a novel nanoparticulate-based formulation encapsulating a HIP complex of lysozyme

Gaudana¹,

Gokulgandhi

Khurana

et al. 2012

Pharmaceutical Development and Technology

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Formulation development of protein therapeutics using polymeric nanoparticles has found very little success in recent years. Major formulation challenges include rapid denaturation, susceptibility to lose bioactivity in presence of organic solvents and poor encapsulation in polymeric matrix. In the present study, we have prepared hydrophobic ion pairing (HIP) complex of lysozyme, a model protein, using dextran sulfate (DS) as a complexing polymer. We have optimized the process of formation and dissociation of HIP complex between lysozyme and DS. The effect of HIP complexation on enzymatic activity of lysozyme was also studied. Nanoparticles were prepared and characterized using spontaneous emulsion solvent diffusion method. Furthermore, we have also investigated release of lysozyme from nanoparticles along with its enzymatic activity. Results of this study indicate that nanoparticles can sustain the release of lysozyme without compromising its enzymatic activity. HIP complexation using a polymer may also be employed to formulate sustained release dosage forms of other macromolecules with enhanced encapsulation efficiency.

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Hemoglobin Denaturation Caused by Surfactants.

Cited by 17 publications

References 0 publications

Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic

Bactericidal Effects and Mechanism of Action of Olanexidine Gluconate, a New Antiseptic

In vitro study of the antioxidant and immunomodulatory activity of aqueous infusion of Bidens pilosa

Design and evaluation of a novel nanoparticulate-based formulation encapsulating a HIP complex of lysozyme

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