Hemoglobin Agenogi (α2β290lys), a slow-moving hemoglobin of a Japanese family resembling Hb-E

Miyaji, T; Suzuki, Haruo; Ohba, Y; Shibata, Susumu

doi:10.1016/0009-8981(66)90187-2

Cited by 46 publications

(4 citation statements)

References 11 publications

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“…Variant hemoglobin was strongly suspected from the dissociation between IA-HbA1c and HPLC-SM-HbA1c in this case. Analysis of the globin gene revealed a heterozygous mutation at β codon 90 of Glu (GAG) → Lys (AAG) and the patient was diagnosed with Hb Agenogi [11].…”

Section: Resultsmentioning

confidence: 99%

“…1d). As Hb Agenogi is slow-moving variant hemoglobin [11], HPLC-VM-HbA1c is more likely to produce a correct value. HPLC-VM-HbA1c measured using 4 different instruments was 6.4 ± 0.2% (mean ± SD).…”

Section: Resultsmentioning

confidence: 99%

“…Falsely high-immunoassay results with methods using the Fujirebio antibody and falsely low HPLC in standard mode were observed in a case of Hb Agenogi. Hb Agenogi is asymptomatic variant hemoglobin which was first reported in 1966 by Miyaji et al [11]. They already reported that Hb Agenogi is slow-moving variant hemoglobin.…”

Section: Discussionmentioning

confidence: 96%

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A case of variant hemoglobin (Hb Agenogi) with type 2 diabetes mellitus showed high HbA1c levels measured by immunoassay due to enhanced antigenicity

Yamane¹,

Tsumori²,

Ishibashi

et al. 2018

Diabetol Int

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HbA1c is a widely utilized biomarker for the management of diabetes mellitus. The presence of variant hemoglobins might interfere with HbA1c measurement using different methods. We herein describe Hb Agenogi (β90Glu → Lys) with type 2 diabetes mellitus whose HbA1c measured by immunoassay (IA) showed falsely high levels while HbA1c measured by highperformance liquid chromatography (HPLC) in the standard mode (SM) showed falsely low levels. To clarify the cause of the falsely high-IA-HbA1c levels, HbA1c was measured by various methods. Glycated albumin was slightly higher than the reference range. HbA1c measured by HPLC in the variant mode, affinity assay and enzymatic assay showed the range (mean ± 2SD: 6.0-6.8%) in this case. However, HbA1c measured by several HPLC-SMs showed falsely low levels (4.1-4.4%). IA-HbA1c using an antibody manufactured by Fujirebio Inc. showed falsely high levels (7.3-8.2%); whereas, IA-HbA1c using an antibody manufactured by Roche Ltd. showed the expected range (6.2-6.5%). In the case of Hb Agenogi, IA-HbA1c using an antibody manufactured by Fujirebio yielded falsely high levels. The mutation at codon 90 of the β-globin chain might enhance antigenicity of the N-terminal peptide region and, therefore, lead to falsely high-HbA1c levels in IA-HbA1c.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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A case of variant hemoglobin (Hb Agenogi) with type 2 diabetes mellitus showed high HbA1c levels measured by immunoassay due to enhanced antigenicity

Yamane¹,

Tsumori²,

Ishibashi

et al. 2018

Diabetol Int

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show abstract

“…Although external, there is no involvement of the side chain in α 1 β 1 contacts and it is not clear how the mutation alters O 2 affinity. Surprisingly, the conservative mutation of glutamic to aspartic acid produces an increased oxygen affinity in Hb Pierre–Benite, while the gross charge changes associated with Glu→Lys and Glu→Gly β90 mutations led to small decreases in affinity in Hbs Agenogi and Roseau‐Pointe‐a‐Pitre, respectively (Miyaji et al ., 1966; Merault et al ., 1985). Baklouti et al .…”

Section: Resultsmentioning

confidence: 99%

Haemoglobin Pierre-Benite - a high affinity variant associated with relative polycythaemia

Beard

Potter

Spearing

et al. 2001

Clinical &Amp; Laboratory Haematology

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This is the second reported example of Hb Pierre--Benite (beta90 Glu-->Asp). This mutation is associated with increased oxygen affinity and polycythaemia. No instability was found and there was no charge shift detected by cellulose acetate electrophoresis at pH 8.3. The mutation was however, clearly indicated by electrospray ionization mass spectrometry (ESI MS), which showed an abnormal beta chain with a 14 Da decrease in mass. Blood volume studies documented a relative rather than a true polycythaemia and this finding has been reported in at least two other high affinity haemoglobin variants--Hb Heathrow and Hb Rahere. This finding led to delay in diagnosis because high oxygen affinity variants are conventionally considered to cause a true polycythaemia.

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A compilation of amino acid analyses of proteins

Kirschenbaum

1975

Analytical Biochemistry

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Hemoglobin Agenogi (α2β290lys), a slow-moving hemoglobin of a Japanese family resembling Hb-E

Cited by 46 publications

References 11 publications

A case of variant hemoglobin (Hb Agenogi) with type 2 diabetes mellitus showed high HbA1c levels measured by immunoassay due to enhanced antigenicity

A case of variant hemoglobin (Hb Agenogi) with type 2 diabetes mellitus showed high HbA1c levels measured by immunoassay due to enhanced antigenicity

Haemoglobin Pierre-Benite - a high affinity variant associated with relative polycythaemia

A compilation of amino acid analyses of proteins

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