Hemodynamic Study of Penile Erection in Dogs

Lin, Shinn Nan; Wang, Jin Min; Cheng, Ping; Chen, Hsing I.

doi:10.1159/000472550

Cited by 13 publications

(5 citation statements)

References 6 publications

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“…Electrical stimulation of the cavernous nerve has been widely used to induce penile erection in various animal models [1–9]. Studies in these models have greatly advanced our understanding of the basic mechanisms, clinical relevance, and pharmacological regulation of penile erection in humans [10–18].…”

Section: Introductionmentioning

confidence: 99%

Metrics for Evaluation of Age-Related Changes in Erectile Capacity in a Rodent Model

Zhao

Sato

Melman

et al. 2009

The Journal of Sexual Medicine

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Introduction Stimulation of the cavernous nerve elicits increases in intracavernosal pressure (ICP) and visible penile erection (VPE) in rats. The observed penile erection can be characterized by the change in ICP and the ratio of ICP over blood pressure (BP; systemic blood pressure). Aim We evaluated correlations between ICP, BP, ICP/BP, and VPE, and the magnitude of the nerve stimulation required to elicit those responses in order to evaluate age-related changes in erectile function in a rodent model. Methods Nine young (3 months) and 10 old (18 months) rats were used. Under anesthesia cavernous nerve stimulation was performed at stimulation strengths ranging from 0.1 to 10 mA. Main Outcome Measures The ICP, BP, ICP/BP, and VPE responses to cavernous nerve stimulation were quantified and compared. Results Significant correlations were observed between ICP and ICP/BP in both young (r2 = 0.886) and old (r2 = 0.962) rats. If the ratio ICP/BP was taken as a metric of VPE and non-erection, then the threshold value for observing a VPE in the majority of animals was ≥0.5 in both young and old rats. However, higher current stimulation was required in the old than in the young animals to achieve erections. Moreover, both the maximal ICP and ICP/BP ratios achieved during erection were also lower in the old (85 ± 2 cmH2O and 0.6 ± 0.02, respectively) than young (106 ± 3 cmH2O and 0.75 ± 0.02, respectively) rats (P < 0.0001). Conclusion Old rats without obvious comorbidities were capable of penile erections, but those erections were accompanied by reduced ICP responses to cavernous nerve stimulation, implying apparently reduced penile rigidity. Taken together our current observations indicate that ICP, ICP/BP, and VPE provide useful metrics/indices of erectile capacity/function, and, moreover, that improved understanding of the inter-relationships among these parameters provides a frame work for further exploration of the mechanistic basis for age-related erectile dysfunction.

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Section: Introductionmentioning

confidence: 99%

Metrics for Evaluation of Age-Related Changes in Erectile Capacity in a Rodent Model

Zhao

Sato

Melman

et al. 2009

The Journal of Sexual Medicine

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“…The corpora cavernosa of the dog has been shown to be both anatomically and physiologically similar to that of the human penis. 4–6 In the present study we have used a dog model to determine the clinical utility of cannulating the penile corpora cavernosa as a means of venous access for fluid resuscitation and drug administration. We have attempted to measure maximal flow rates and assess whether these are sufficient for use in volume restoration in hypovolaemic shock.…”

Section: Introductionmentioning

confidence: 99%

Corpus Cavernosum as an Alternative Means of Intravenous Access in the Emergency Setting

Nicol

Watt

Wood

et al. 2000

Aust. N.Z. J. Surg.

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“…Many experimental animals have been employed in in vivo studies of penile erection, such as dogs, monkeys, cats, rats and rabbits (Chen et al, 1992;Domer et al, 1978;Lin et al, 1985;Lue et al, 1983;Stacle et al, 1988). Lin & Lin (1996) reported that the e ects of vasoactive drugs on the rabbit corpus cavernosum are similar to those in humans; thus the rabbit model is a suitable alternative for further physiological and pharmacological studies of penile erection.…”

Section: Introductionmentioning

confidence: 99%

Relaxation of corpus cavernosum and raised intracavernous pressure by berberine in rabbit

Chiou

Chen²,

Chen

1998

British J Pharmacology

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1 In the present study, we have investigated the e ect of berberine in rabbit isolated corpus cavernosum and measured the intracavernous pressure (ICP) change after intracavernosal injection of berberine in rabbit. 2 Berberine alone suppressed the basal tone and induced a concentration (0.1 ± 100 mM)-dependent relaxation in phenylephrine (PE)-precontracted corpus cavernosum. 3 Tetrodotoxin (0.1 and 1 mM) treatment had no signi®cant e ect on the berberine-induced relaxation. Phentolamine (1 and 10 mM), propranolol (1 and 3 mM) and atropine (1 and 3 mM) were also without e ect. These results suggest that berberine might cause relaxation of the cavernosal strip by direct action on the corpus cavernosum, not by a neuronal e ect. Furthermore, muscarinicand b-adrenoceptors were not involved. 4 Berberine-induced relaxations were signi®cantly reduced by endothelium removal and by exposure to L-N G -nitro arginine methyl ester (0.1 and 0.3 mM), but not indomethacin (30 mM). 5 In endothelium-deprived corpus cavernosal tissues, berberine-induced relaxations were signi®cantly reduced in high K + medium (KCl=60 mM), by charybdotoxin (ChTX) and 4-aminopyridine (4-AP) but not by glibenclamide and apamin. 6 After intracavernous injection of berberine (1, 2, 3 and 5 mg kg 71 ), the ICP rose from 12.7+3.6 to 13.2+5.4, 25.3+6.1, 46.5+8.2, and 63.4+10.2 mmHg, respectively. The duration of tumescence ranged from 11.5 ± 43.7 min. 7 The results show that berberine possesses a relaxant e ect on rabbit corpus cavernosal tissues which is attributable to both endothelium-dependent and-independent properties. While the former component is apparently due to the release of NO from sinusoidal endothelium, the endotheliumindependent mechanism involved in berberine relaxation is probably linked to ChTX-and 4-APsensitive K + channel activation in the cavernosal vasculature.

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Hemodynamic Study of Penile Erection in Dogs

Cited by 13 publications

References 6 publications

Metrics for Evaluation of Age-Related Changes in Erectile Capacity in a Rodent Model

Metrics for Evaluation of Age-Related Changes in Erectile Capacity in a Rodent Model

Corpus Cavernosum as an Alternative Means of Intravenous Access in the Emergency Setting

Relaxation of corpus cavernosum and raised intracavernous pressure by berberine in rabbit

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