Hemodynamic Risk Factors for the Development of Carotid Stenosis in Patients with Unilateral Carotid Stenosis

Enomoto, Riku; Hasegawa, Koji; Yasoda, Akihiro; Tsukahara, Tetsuya; Shimogonya, Yuji; Yonemoto, Naohiro; Fukuda, Shunichi; Ezura, Masayuki; Kurihara, Hideyuki; Furumoto, Hideharu; Tanno, Hirokazu; Masaoka, Hiroyuki; Saito, Ryoichi; Sakai, Keiichi; Odake, Yasuko; Shoin, Katsuo; Fujisawa, Hironori; Suzaki, Noriyuki; Sakai, Hideki; Yamada, Tatsuya; Adachi, Yoshiki; Manabe, Yasuhiro; Torii, Tsuyoshi; Sadatomo, Takashi; Izumihara, Akifumi; Yamashita, Katsuhiro; Okada, Yasushi; Nakane, Hiroshi; Murata, Masayuki; Tsutsumi, Keisuke; Takita, Tomohiro; Kosaki, Yanosuke; Nagai, Yasuyuki; Iihara, Koji; Izumo, Tsuyoshi; Sakai, Nobuyuki; Suyama, Kazuhiko; Oogami, Hisako; Fukuda, Miyuki; Watanabe, Aoi; Fujiwara, Kazuha; Nakajo, Natsuko; Kikuchi, Shunsuke

doi:10.1016/j.wneu.2022.01.019

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…NtransWSS represents the degree of disturbance in the strength and direction of the shear stress, with a maximum value of 1.0. Since disturbed flow with an NtransWSS ranging from 0.2 to 1.0 has been shown to occur in human carotid bifurcations where atherosclerotic plaques are prone to develop ( 24 ), we subjected the cells to a disturbed flow with an NtransWSS of 0.2 or more (average, 0.4) and an average WSS of 20 dynes/cm 2 by adjusting the step height and flow rate ( Fig. 1 A ).…”

Section: Methodsmentioning

confidence: 99%

Endothelial cells differentially sense laminar and disturbed flows by altering the lipid order of their plasma and mitochondrial membranes

Yamamoto,

Shimogonya,

Maeno

et al. 2023

American Journal of Physiology-Cell Physiology

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Endothelial cells (ECs) experience two different blood flow patterns: laminar and disturbed flow. Their responses to laminar flow contribute to vascular homeostasis, whereas their responses to disturbed flow result in EC dysfunction and vascular diseases. However, it remains unclear how ECs differentially sense laminar and disturbed flow and trigger signaling that elicits different responses. Here, we showed that ECs differentially sense laminar and disturbed flows by altering the lipid order of their plasma and mitochondrial membranes in opposite directions. This results in distinct changes in mitochondrial function, namely, increased adenosine triphosphate (ATP) production for laminar flow and increased hydrogen peroxide (H2O2) release for disturbed flow, leading to ATP- and H2O2-mediated signaling, respectively. When cultured human aortic ECs were subjected to laminar or disturbed flow in flow-loading devices, the lipid order of their plasma membranes immediately decreased in response to laminar flow and increased in response to disturbed flow. Laminar flow also decreased the lipid order of mitochondrial membranes and increased mitochondrial ATP production. In contrast, disturbed flow increased the lipid order of mitochondrial membranes and increased the release of H2O2 from the mitochondria. The addition of cholesterol to the cells increased the lipid order of both membranes and abrogated laminar flow-induced ATP production, while treatment of the cells with a cholesterol-depleting reagent, methyl-β cyclodextrin, decreased the lipid order of both membranes and abolishτed disturbed flow-induced H2O2 release, indicating that changes in the membrane lipid order and/or cholesterol content are closely linked to flow-induced changes in mitochondrial functions.

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Section: Methodsmentioning

confidence: 99%

Endothelial cells differentially sense laminar and disturbed flows by altering the lipid order of their plasma and mitochondrial membranes

Yamamoto,

Shimogonya,

Maeno

et al. 2023

American Journal of Physiology-Cell Physiology

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“…The formation of atherosclerotic plaques entails violation of blood vessel patency and deterioration in the blood supply of tissues and organs. Atherosclerosis of the internal carotid artery leads to carotid stenosis [ 1 ], which is the cause of ischemic stroke in 15% of cases [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

New Tissue-Engineered Vascular Matrix Based on Regenerated Silk Fibroin: in vitro Study

Prokudina,

Senokosova,

Antonova

et al. 2023

Sovrem Tehnol Med

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The aim of the study was to make a vascular patch based on regenerated silk fibroin (SF) and study its physical and mechanical characteristics, biocompatibility and matrix properties in comparison with polyhydroxybutyrate/valerate/polycaprolactone with incorporated vascular endothelial growth factor (PHBV/PCL/VEGF) and commercial bovine xenopericardium (XP) flap in experiments in vitro . Materials and Methods Tissue-engineered matrices were produced by electrospinning. The surface structure, physical and mechanical characteristics, hemocompatibility (erythrocyte hemolysis, aggregation, adhesion and activation of platelets after contact with the material) and matrix properties of vascular patches (adhesion, viability, metabolic activity of EA.hy926 cells on the material) were studied. Results The surface of SF-based matrices and PHBV/PCL/VEGF-based tissue engineered patches had a porous and fibrous structure compared to a denser and more uniform XP flap. The physical and mechanical characteristics of SF matrices were close to those of native vessels. Along with this, tissue-engineered patches demonstrated high hemocompatible properties, which do not differ from those for commercial XP flap. Adhesion, viability, and metabolic activity of EA.hy926 endothelial cells also corresponded to the previously developed PHBV/PCL/VEGF matrix and XP flap, which indicates the nontoxicity and biocompatibility of SF matrices. Conclusion Matrices produced from regenerated SF demonstrated satisfactory results, comparable to those for PHBV/PCL/VEGF and commercial XP flap, and in the case of platelet adhesion and activation, they outperformed these patches. In total, SF can be defined as material having sufficient biological compatibility, which makes it possible to consider a tissue-engineered matrix made from it as promising for implantation into the vascular wall.

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“…Moreover, CAS is reported to account for at least 15-20% of all ischemic strokes [6] and is promoted by hypertension, smoking, and diabetes mellitus (DM) [3,5,7]. In addition, certain hemodynamic features including turbulent blood flow or low wall shear stress also play key roles in CAS progression [8,9]. Furthermore, high levels of total cholesterol (TC) and LDL-C are well-known as risk factors for CAS progression, whereas the relationships between hypertriglyceridemia and CAS are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Hypertriglyceridemia and Atherosclerotic Carotid Artery Stenosis

Miura

Suzuki

2022

IJMS

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Both fasting and non-fasting hypertriglyceridemia have emerged as residual risk factors for atherosclerotic disease. However, it is unclear whether hypertriglyceridemia increases the risks of the progression of carotid artery stenosis. Statins are well known to prevent carotid plaque progression and improve carotid plaque instability. In addition, statin therapy is also known to reduce cerebrovascular events in patients with carotid artery stenosis and to improve clinical outcomes in patients undergoing revascularization procedures. On the other hand, there have been no randomized controlled trials showing that the combination of non-statin lipid-lowering drugs with statins has additional beneficial effects over statin monotherapy to prevent cerebrovascular events and stenosis progression in patients with carotid artery stenosis. In this article, the authors demonstrate the mechanisms of atherosclerosis formation associated with hypertriglyceridemia and the potential role of lipid-lowering drugs on carotid artery stenosis. The authors also review the articles reporting the relationships between hypertriglyceridemia and carotid artery stenosis.

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Hemodynamic Risk Factors for the Development of Carotid Stenosis in Patients with Unilateral Carotid Stenosis

Cited by 6 publications

References 22 publications

Endothelial cells differentially sense laminar and disturbed flows by altering the lipid order of their plasma and mitochondrial membranes

Endothelial cells differentially sense laminar and disturbed flows by altering the lipid order of their plasma and mitochondrial membranes

New Tissue-Engineered Vascular Matrix Based on Regenerated Silk Fibroin: in vitro Study

Hypertriglyceridemia and Atherosclerotic Carotid Artery Stenosis

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