Hemodynamic patterns of phosphatidylcholine hydroperoxide and hyaluronic acid during hepatic ischemia-reperfusion

Abstract

We investigated the hemodynamic pattern of serum hyaluronic acid (HA) and compared it with that of plasma phosphatidylcholine hydroperoxide (PCOOH) in terms of a convenient parameter of reperfusion injury. Using pig models, we designed two continuous ischemia groups, prepared by blockage of the blood flow at the hepatic hilum for 10 or 30 min. A discontinuous ischemia model was prepared by repeating the 10-min ischemia procedure, followed by 10 min of reperfusion, to a total ischemia period of 30 min. The PCOOH level started to increase just after reperfusion and reached the peak at 90 min, followed by a gradual decline after 6 h. The HA level increased rapidly in the continuous ischemia groups, starting immediately after ischemia onset until immediately before reperfusion, followed by a gradual decrease during up to 6 h of reperfusion. The HA levels in the three groups were almost normalized after 90 min of reperfusion, when the PCOOH level reached the peak. These results indicated that the plasma PCOOH level is a useful parameter for predicting the onset and progress of reperfusion injury in its initial stages.

Hepatic cell membrane damage during cold preservation sensitizes liver grafts to rewarming injury

“…Loss of energy leads to an influx of calcium and an activation of phospholipases, which separate glycerol away from lipids in the cell membrane 21. Oxygen‐derived free radicals also contribute to this membrane damage 22. By continuous monitoring of glycerol in the liver it should be possible to estimate the level of cell membrane injury prior to transplantation.…”

“…21 Oxygen-derived free radicals also contribute to this membrane damage. 22 By continuous monitoring of glycerol in the liver it should be possible to estimate the level of cell membrane injury prior to transplantation. We hypothesize that this damage correlates with posttransplant liver function and generally with the outcome.…”

Microdialysis monitoring for evaluation of the influence exerted by pneumoperitoneum on the kidney: an experimental study

“…Loss of energy leads to an influx of calcium and an activation of intracellular phospholipase, which splits glycerol away from the cell membrane. In addition, oxygen-derived free radicals contribute to this membrane damage [16].…”

Transplante hepático large-for-size porcino com arterialização da veia porta: estudo hemodinâmico, histológico, bioquímico e biomolecular

“…O aperfeiçoamento da técnica de arterialização, a expansão dos grupos amostrais, tempos maiores de observação, além da pesquisa de outras variáveis que caracterizem a LIR, estão entre as perspectivas futuras deste grupo de trabalho. A dosagem de glutationa e sua correlação com a presença de radicais oxidativos já foi empregada em outras publicações para a análise da LIR (48) e também pode ser agregar mais informações em trabalhos futuros.…”