Hemodynamic changes in a rat parietal cortex after endothelin-1-induced middle cerebral artery occlusion monitored by optical coherence tomography

Abstract

A blockage of the middle cerebral artery (MCA) on the cortical branch will seriously affect the blood supply of the cerebral cortex. Real-time monitoring of MCA hemodynamic parameters is critical for therapy and rehabilitation. Optical coherence tomography (OCT) is a powerful imaging modality that can produce not only structural images but also functional information on the tissue. We use OCT to detect hemodynamic changes after MCA branch occlusion. We injected a selected dose of endothelin-1 (ET-1) at a depth of 1 mm near the MCA and let the blood vessels follow a process first of occlusion and then of slow reperfusion as realistically as possible to simulate local cerebral ischemia. During this period, we used optical microangiography and Doppler OCT to obtain multiple hemodynamic MCA parameters. The change trend of these parameters from before to after ET-1 injection clearly reflects the dynamic regularity of the MCA. These results show the mechanism of the cerebral ischemia-reperfusion process after a transient middle cerebral artery occlusion and confirm that OCT can be used to monitor hemodynamic parameters.

Optimized depth-resolved estimation to measure optical attenuation coefficients from optical coherence tomography and its application in cerebral damage determination

“…In this study, a spectral-domain OCT (SD-OCT) system was employed, which is similar to our previous work. 11,12 Briefly, a superluminescent diode with a central wavelength of 1310 nm and a bandwidth of 65 nm was used as a light source, providing an ∼8.9-μm axial resolution in the tissue. In the sample arm, an achromatic lens with 30-mm focal length was used to achieve theoretical lateral resolution of ∼9 μm.…”

“…The model of the focal cerebral ischemia used in our experiments was based on the previously described endothelin-1- induced middle cerebral artery occlusion (MCAO) model. 12 The stereotaxic coordinates of the ET-1 injection were −0.83-mm anterior, −3.36-mm lateral, and 1.0-mm ventral relative to bregma. A dose of 3-μL ET-1 (dilute to 100 pmol/L in 0.9% normal saline) was injected at 1 μL∕ min.…”

In vivo monitoring of thrombosis in mice by optical coherence tomography

“…This OCT system uses near‐infrared light. Because the frequencies and bandwidths of infrared light are orders of magnitude higher than medical ultrasound signals, which results in greater image resolution, OCT enables imaging capillaries in mice .…”

“…OCT angiography (OCTA) is a functional extension of OCT and is able to provide 3D blood perfusion map in tissue down to capillary level without a need for contrast agents . OCTA has been successfully used to image micro‐vascular networks in a variety of tissues in vivo, for example, brain , retina , skin , skeletal muscles and microvascular anastomosis .…”

Simultaneous detection of cerebral blood perfusion and cerebral edema using swept‐source optical coherence tomography

“…The model of cerebral ischemia used in our experiments was based on endothelin-1-induced MCAO model described previously [31]. The stereotaxic coordinates of the ET-1 (Shanghai Jianglai industrial Limited by Share Ltd. China) injection were 0.9 mm anterior, 5.2 mm lateral and 8.7 mm ventral relative to bregma.…”