Hemodialysis-activated granulocytes at the site of interstitial inflammation

Jacobson, Stefan H.; Thylén, Pia; Fernvik, Eva; Halldén, Gunilla; Grönneberg, R.; Lundahl, Joachim

doi:10.1053/ajkd.2002.32008

Cited by 8 publications

(19 citation statements)

References 26 publications

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“…Autologous serum was collected the day before and immediately frozen at -70 ° C. Administration of serum or PBS was done in order to induce a site of intense inflammation and a site of intermediate inflammation, respectively. Previously published data show that autologous serum induces a more intense inflammation than stimulation with PBS [11,13] . The intraindividual reproducibility of this in vivo method is acceptable and comparable to in vitro methods for transmigration [14] .…”

Section: Skin Suction Chambers and Collection Of Blister Fluidmentioning

confidence: 94%

“…This method has previously been described in detail [11][12][13] . Briefly, a skin suction chamber technique was used to induce an interstitial inflammation on the volar surface of the forearm in patients and healthy subjects.…”

Section: Skin Suction Chambers and Collection Of Blister Fluidmentioning

confidence: 99%

“…We have previously studied monocyte and neutrophil activation and transendothelial migration into induced sites of inflammation in the interstitium in patients on low-flux bioincompatible HD and in patients on highflux hemodiafiltration (HDF) [11][12][13] . In these studies, we demonstrated that monocytes and neutrophils collected from patients on low-flux bioincompatible HD have an impaired capacity to upregulate CD11b at the site of interstitial inflammation, which is considered their actual site of action.…”

Section: Introductionmentioning

confidence: 99%

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Monocyte and Neutrophil Chemotactic Activity at the Site of Interstitial Inflammation in Patients on High-Flux Hemodialysis or Hemodiafiltration

Olsson

Paulsson²,

Dadfar³

et al. 2009

Blood Purif

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Background/Aims: We have observed a difference between patients on low-flux hemodialysis (HD) or peritoneal dialysis and patients on hemodiafiltration (HDF) or high-flux HD in the capacity of transmigrated leukocytes to mobilize CD11b in response to inflammatory stimuli compared with healthy subjects. This could be due to different interstitial chemokine concentrations. Methods: We measured concentrations of circulating and interstitial macrophage inflammatory protein-1α (MIP-1α), matrix metalloproteinase-9 (MMP-9)/neutrophil gelatinase-associated lipocalin (NGAL), monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in 10 patients on HDF or high-flux HD and 11 healthy subjects by using immunoassay. Results: The interstitial concentrations of MIP-1α, MMP-9/NGAL and IL-8 were similar in patients and healthy subjects, while the corresponding concentration of MCP-1 was significantly higher in patients on HDF or high-flux HD as compared with healthy subjects (p < 0.01). Conclusion: We suggest that an equal or higher concentration of chemokines in the interstitium in patients with HDF or high-flux HD might be one mechanism responsible for the preserved function of transmigrated leukocytes.

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Section: Skin Suction Chambers and Collection Of Blister Fluidmentioning

confidence: 94%

Section: Skin Suction Chambers and Collection Of Blister Fluidmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monocyte and Neutrophil Chemotactic Activity at the Site of Interstitial Inflammation in Patients on High-Flux Hemodialysis or Hemodiafiltration

Olsson

Paulsson²,

Dadfar³

et al. 2009

Blood Purif

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“…The method used was the skin chamber technique, which is well documented and has been used by a number of investigators to study transmigration and recruitment of leukocytes at the inflammatory site (Scheja & Forsgren 1985;Follin 1999;Thylen et al 2000;Jacobson et al 2002;Theilgaard-Monch et al 2004;Dadfar et al 2007;Paulsson et al 2007). With the skin chamber technique, we measured leukocyte functions at time 0 (before the high-flux hemodialysis/hemodiafiltration session) and after 10 hours (within which time the highflux hemodialysis/hemodiafiltration treatment was performed).…”

Section: Methods For Leukocyte Functional Studiesmentioning

confidence: 99%

“…Highflux hemodialysis causes lower levels of IL-6 and IL-1 than low-flux hemodialysis or dialysis with cuprophane membranes (Schindler et al 2006). Our group has previously demonstrated that neutrophils and monocytes recruited to an induced interstitial inflammatory site in patients treated with low-flux bioincompatible hemodialysis have an impaired capacity of mobilizing CD11b in response to the induced inflammation, compared with the corresponding cells from healthy subjects Jacobson et al 2002). Chronic kidney disease is a state that induces apoptosis, but this is normalized with continuous and high-flux hemodialysis modalities (D'Intini et al 2004;Bordoni et al 2006).…”

Section: Patients On Hemodialysismentioning

confidence: 97%

Leukocyte Function in High-Flux Hemodialysis

Olsson¹

2011

Progress in Hemodialysis - From Emergent Biotechnology to Clinical Practice

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Preserved leukocyte CD11b expression at the site of interstitial inflammation in patients with high-flux hemodiafiltration

Olsson

Dadfar

Paulsson

et al. 2007

Kidney International

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The impact of high-flux hemodialysis on clinical outcomes remains controversial. We have previously shown that in vivo transmigrated leukocytes from patients with low-flux bioincompatible hemodialysis have an impaired capacity to upregulate CD11b at the site of interstitial inflammation. In the present study, we investigated the in vivo capacity of transmigrated monocytes and granulocytes to express CD11b at the site of interstitial inflammation in 10 patients on biocompatible high-flux hemodiafiltration or high-flux hemodialysis and 12 healthy subjects, and the in vitro response to a bacteria-related peptide (N-formyl-methionyl-leucyl-phenylalanine (fMLP)). Leukocyte formation of hydrogen peroxide (H(2)O(2)) and leukocyte apoptosis were also studied. In patients, both monocytes and granulocytes had a preserved capacity to express CD11b following in vivo transmigration to sites of interstitial inflammation, compared with cells from healthy subjects. Furthermore, monocytes and granulocytes from patients showed a preserved ability to respond to challenge with fMLP in the extravascular milieu. The intracellular killing capacity of leukocytes (H(2)O(2) production) in the interstitium was similar as of cells from healthy subjects both before and after stimulation with fMLP. Following maximal receptor independent stimulation (phorbol 12-myristate 13-acetate), leukocytes from patients showed lower H(2)O(2) production at the site of intense inflammation, compared with cells from healthy subjects. Finally, leukocyte apoptosis in interstitial inflammation was similar in patients and healthy subjects. We conclude that in vivo transmigrated leukocytes from patients on biocompatible high-flux hemodiafiltration or high-flux hemodialysis have a preserved capacity to express CD11b at the site of interstitial inflammation. This may have important biological implications.

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Hemodialysis-activated granulocytes at the site of interstitial inflammation

Cited by 8 publications

References 26 publications

Monocyte and Neutrophil Chemotactic Activity at the Site of Interstitial Inflammation in Patients on High-Flux Hemodialysis or Hemodiafiltration

Monocyte and Neutrophil Chemotactic Activity at the Site of Interstitial Inflammation in Patients on High-Flux Hemodialysis or Hemodiafiltration

Leukocyte Function in High-Flux Hemodialysis

Preserved leukocyte CD11b expression at the site of interstitial inflammation in patients with high-flux hemodiafiltration

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