Hemocytometer Counting

Absher, Marlene

doi:10.1016/b978-0-12-427150-0.50098-x

Cited by 81 publications

(37 citation statements)

References 3 publications

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“…Alternatively, these parameters could be related to cell numbers, but cell counting is less accurate than DNA measurements [1]. The constant cell division/DNA amount 399 confirms a stable growth rate.…”

Section: Discussionmentioning

confidence: 99%

Quantification of in vitro growth and synthesis in rabbit middle ear epithelial cells

Schousboe

Blegvad

Ovesen

1996

Eur Arch Otorhinolaryngol

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The purpose of the present study was a quantitative description of middle ear epithelial cell reactions in a standardized model system under controlled conditions. The model is based on a newly developed culture method for rabbit middle ear epithelial cells. The growth pattern of identical subcultures was determined by measurement of total cell protein, DNA synthesis and DNA amount during a 15-day period. The secretory product was quantified by means of 3H-glucosamine incorporation. Epithelial cells from the auditory meatus were examined by identical methods. The results proved the method to be reproducible regarding all parameters. The DNA amount and total cell protein increased throughout the period, whereas DNA synthesis remained constant. Secretion increased linearly for 9 days and then diminished. All parameters were without significant changes when related to DNA amount, reflecting a uniform cellular activity throughout the period. The DNA amount was the most precise parameter with a mean variation coefficient of 6.0% (SD 3.1%). With the baseline values found to be reproducible for the parameters chosen, our model system is considered to be usable for quantifying various factors influencing the proliferation and activity of middle ear epithelium.

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Section: Discussionmentioning

confidence: 99%

Quantification of in vitro growth and synthesis in rabbit middle ear epithelial cells

Schousboe

Blegvad

Ovesen

1996

Eur Arch Otorhinolaryngol

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“…The cell suspension was then serially diluted using LB broth. For each dilution, the OD at 600 nm was measured using the spectrophotometer and the cell count [17] was determined using a Neubauer hemocytometer counting chamber (Clay Adams) under an optical microscope (Nikon, Optiphot). Figure 1b shows an example of a calibration curve generated for E. coli (K12).…”

Section: Determining Cell Concentrationmentioning

confidence: 99%

SERS substrates fabricated using ceramic filters for the detection of bacteria

Mosier‐Boss

Sørensen

George

et al. 2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…Viable cell numbers were estimated using the method of Absher [26]. Cell suspensions were adjusted to 5 10 7 cells ml 1 , and 100 l well 1 plated into sterile tissue culture plates and sterile chamber slides (Nunc).…”

Section: Extraction Of Kidney Macrophagesmentioning

confidence: 99%