Hemocytes of the Australian Red Claw Crayfish (Cherax quadricarinatus): Morphology and Hemogram

Skafar, D.; Shumeiko, D. V.

doi:10.1134/s0022093022060060

Cited by 5 publications

(1 citation statement)

References 36 publications

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“…After 1.5 h, 1 and 3 days hemolymph was extracted from amphipods and mixed 1:1 with adjusted anticoagulant solution (150 mM NaCl, 5 mM Na 2 HPO 4 , 30 mM sodium citrate, 10 mM EDTA, 50 mM EDTA-Na 2 , pH 8.0). This adjusted anticoagulant solution allows to fix hemocytes in the state when nuclei and granules are visible more clearly ( Skafar & Shumeiko, 2022 ) and was applied to later visually distinguish granulocytes among all hemocytes. THC and granulocyte proportion were estimated under the Mikmed-2 microscope in a glass hemocytometer.…”

Section: Methodsmentioning

confidence: 99%

Leeches Baicalobdella torquata feed on hemolymph but have a low effect on the cellular immune response of amphipod Eulimnogammarus verrucosus from Lake Baikal

Nazarova,

Mutin,

Skafar

et al. 2024

PeerJ

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Lake Baikal is one of the largest and oldest freshwater reservoirs on the planet with a huge endemic diversity of amphipods (Amphipoda, Crustacea). These crustaceans have various symbiotic relationships, including the rarely described phenomenon of leech parasitism on amphipods. It is known that leeches feeding on hemolymph of crustacean hosts can influence their physiology, especially under stressful conditions. Here we show that leeches Baicalobdella torquata (Grube, 1871) found on gills of Eulimnogammarus verrucosus (Gerstfeldt, 1858), one of the most abundant amphipods in the Baikal littoral zone, indeed feed on the hemolymph of their host. However, the leech infection had no effect on immune parameters such as hemocyte concentration or phenoloxidase activity and also did not affect glycogen content. The intensity of hemocyte reaction to foreign bodies in a primary culture was identical between leech-free and leech-infected animals. Artificial infection with leeches also had only a subtle effect on the course of a model microbial infection in terms of hemocyte concentration and composition. Despite we cannot fully exclude deleterious effects of the parasites, our study indicates a low influence of a few leeches on E. verrucosus and shows that leech-infected amphipods can be used at least for some types of ecophysiological experiments.

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Section: Methodsmentioning

confidence: 99%