Hemocytes of bivalve mollusks as cellular models in toxicological studies of metals and metal-based nanomaterials

Weng, Nanyan; Meng, Jie; Huo, Shouliang; Wu, Fengchang; Wang, Wen‐Xiong

doi:10.1016/j.envpol.2022.120082

Cited by 11 publications

(7 citation statements)

References 153 publications

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“…3 As a sensitive and unique cellular model, they have been widely used to investigate the immunological effects of various metals or metal nanoparticles. 4 Cu is a crucial trace metal necessary for various innate immune functions. 5 It can be accumulated to extraordinarily high concentrations in oysters even at low ambient concentrations, and can be retained within oysters for a long time.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Oyster hemocytes can be classified into three cellular subpopulations due to their high heterogeneity of morphology, structure, and functions. 4 Based on the intracellular granularity (primarily determined by the numbers and size of lysosomes), oyster hemocytes were divided into agranulocytes, semigranulocytes, and granulocytes. 11 Agranulocytes have few cytoplasmic granules, and are considered as blast-like cells without the Golgi apparatus and endoplasmic reticulum.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Consequently, hemocytes come into contact with many different cells and tissues, which provides the opportunity to remove metals for sequestration in lysosomes . As a sensitive and unique cellular model, they have been widely used to investigate the immunological effects of various metals or metal nanoparticles …”

Section: Introductionmentioning

confidence: 99%

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Lysosomal Cu(I)/Cu(II) Dependence of Antimicrobial Ability of Oyster Hemocytes and Regulation of Phagolysosomal System

Luo,

Wang

2023

Environ. Sci. Technol.

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Copper (Cu) is hyperaccumulated in oyster hemocytes and is an essential trace metal indispensable for diverse innate immune functions. However, the roles of Cu in oyster immune defense are still unclear. In this study, Cu exposure enhanced the phagocytosis of zymosan by increasing the number and length of filopodia, as well as mitochondrial ROS (mitoROS) production mainly in granulocytes, followed by semigranulocytes and agranulocytes. The intracellular calcium level increased to promote the phagosome−lysosome fusion after Cu exposure. The enhancement of phagosomal acidification and mitochondrion−phagosome juxtaposition were also found in granulocytes after Cu exposure. These results indicated that Cu could regulate the phagolysosomal system to enhance the antimicrobial ability of oyster hemocytes with the assistance of mitoROS. Furthermore, Cu(I) and Cu(II) were predominately located in lysosomes, and degranulation may provide a mechanism for exposing Cu to bacteria to prevent their survival and proliferation. Specifically, we showed that the newly formed Cu(I) arising from lysosomal Cu(II) moved to lysosomes and mitochondria in activated hemocytes to induce strong immune responses. The ability of the transformation of Cu(I) from Cu(II) followed granulocytes > semigranlocytes > agranulocytes, indicating that granulocytes played important roles in immune functions of oysters. Our results provided new insights into the understanding of antimicrobial effects of Cu in oyster hemocytes.

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Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Lysosomal Cu(I)/Cu(II) Dependence of Antimicrobial Ability of Oyster Hemocytes and Regulation of Phagolysosomal System

Luo,

Wang

2023

Environ. Sci. Technol.

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“…12,13 R. philippinarum, like many counterparts, leans on its inherent immune arsenal, particularly hemocytes, to defend against xenobiotics. 14 Hemocytes offer a robust framework for probing toxic responses, 15,16 providing deep insights into the potential damage and toxic mechanism by NPs at the cellular level in marine zoobenthos.…”

mentioning

confidence: 99%

“…philippinarum), stands as a representative zoobenthos, widely utilized in toxicity assays owing to its filter-feeding habits, ecological value, and contaminant vulnerability. , R. philippinarum, like many counterparts, leans on its inherent immune arsenal, particularly hemocytes, to defend against xenobiotics . Hemocytes offer a robust framework for probing toxic responses, , providing deep insights into the potential damage and toxic mechanism by NPs at the cellular level in marine zoobenthos.…”

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confidence: 99%

Surface-Charge-Driven Ferroptosis and Mitochondrial Dysfunction Is Involved in Toxicity Diversity in the Marine Bivalve Exposed to Nanoplastics

Zhou,

Liu,

Jiang

et al. 2024

ACS Nano

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Nanoplastics (NPs) pervade daily life, posing serious threats to marine ecosystems. Despite the crucial role that surface charge plays in NP effects, there is a substantial gap in our understanding of how surface charge influences NP toxicity. Herein, by exposing Ruditapes philippinarum (R. philippinarum) to both positively charged NPs (p-NPs) and negatively charged NPs (n-NPs) at environmentally relevant particle number levels for a duration of 35 days, we unequivocally demonstrate that both types of NPs had discernible impacts on the clams depending on their surface charge. Through transcriptomic and proteomic analyses, we unveiled the primary mechanisms behind p-NP toxicity, which stem from induced mitochondrial dysfunction and ferroptosis. In contrast, n-NPs predominantly stimulated innate immune responses, influencing salivary secretion and modulating the complement and coagulation cascades. Furthermore, in vitro tests on clam immune cells confirmed that internalized p-NPs triggered alterations in mitochondrial morphology, a decrease in membrane potential, and the initiation of ferroptosis. Conversely, n-NPs, to a certain extent, moderated the expression of genes related to immune responses, thus mitigating their adverse effects. Taken together, these findings indicate that the differential surface-charge-driven ferroptosis and mitochondrial dysfunction in clams play a critical role in the toxicity profile of NPs, providing an insightful reference for assessing the ecological toxicity associated with NPs.

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Toxicity of environmental and polystyrene plastic particles on the bivalve Corbicula fluminea: focus on the molecular responses

Latchere,

Roman,

Métais

et al. 2024

Ecotoxicology

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Hemocytes of bivalve mollusks as cellular models in toxicological studies of metals and metal-based nanomaterials

Cited by 11 publications

References 153 publications

Lysosomal Cu(I)/Cu(II) Dependence of Antimicrobial Ability of Oyster Hemocytes and Regulation of Phagolysosomal System

Lysosomal Cu(I)/Cu(II) Dependence of Antimicrobial Ability of Oyster Hemocytes and Regulation of Phagolysosomal System

Surface-Charge-Driven Ferroptosis and Mitochondrial Dysfunction Is Involved in Toxicity Diversity in the Marine Bivalve Exposed to Nanoplastics

Toxicity of environmental and polystyrene plastic particles on the bivalve Corbicula fluminea: focus on the molecular responses

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