Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-seq

Hersperger, Fabian; Kastl, Melanie; Paeschke, Katrin; Kierdorf, Katrin

doi:10.1007/978-1-0716-3437-0_4

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…Doublet exclusion was performed via gating on DRAQ7 single positive nuclei. DRAQ7 + DAPI + double positive events were considered to be nuclei and were sorted for the snRNA-seq as previously described ( Hersperger et al, 2024 ). ( B ) Violin plot indicating number of genes identified in analyzed nuclei of the sorted samples.…”

Section: Resultsmentioning

confidence: 99%

DNA damage signaling in Drosophila macrophages modulates systemic cytokine levels in response to oxidative stress

Hersperger

Meyring

Weber

et al. 2024

eLife

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Environmental factors, infection, or injury, cause oxidative stress in diverse tissues, resulting in immune activation and loss of tissue homeostasis. Effective stress response cascades, conserved from invertebrates to mammals, ensure reestablishment of homeostasis and tissue repair. Plasmatocytes, the Drosophila macrophage-like cells, are thought to respond to oxidative stress by immune activation, however the signaling cascades involved in oxidative stress sensing and subsequent immune activation are yet to be defined. Furthermore, their role in modulating and controlling oxidative stress response to facilitate tissue repair and survival of the organism is not resolved. Here we describe the responses of hemocytes in adult Drosophila to oxidative stress and the essential role of non-canonical DNA damage repair activity in direct “responder” hemocytes to control JNK-mediated stress signaling, systemic levels of the cytokine upd3 and subsequently susceptibility to oxidative stress. Our results point to an essential systemic role of hemocytes in controlling systemic oxidative stress response in Drosophila, including energy mobilization for potential tissue repair.

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Section: Resultsmentioning

confidence: 99%

DNA damage signaling in Drosophila macrophages modulates systemic cytokine levels in response to oxidative stress

Hersperger

Meyring

Weber

et al. 2024

eLife

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“…Doublet exclusion was performed via gating on DR single positive nuclei. DR + DAPI + double positive events were considered to be nuclei and were sorted for the snRNA-seq as previously described 49 . (B) Violin plot indicating number of genes identified in analyzed nuclei of the sorted samples.…”

Section: Supplementary Figure Legendsmentioning

confidence: 99%

DNA damage signaling inDrosophilamacrophages modulates systemic cytokine levels in response to oxidative stress

Hersperger

Meyring

Weber

et al. 2023

Preprint

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Environmental factors, infection, or injury, cause oxidative stress in diverse tissues, resulting in immune activation and loss of tissue homeostasis. Effective stress response cascades, conserved from invertebrates to mammals, ensure reestablishment of homeostasis and tissue repair. Plasmatocytes, theDrosophilamacrophage-like cells, are thought to respond to oxidative stress by immune activation, however the signaling cascades involved in oxidative stress sensing and subsequent immune activation are yet to be defined. Furthermore, their role in modulating and controlling oxidative stress response to facilitate tissue repair and survival of the organism is not resolved. Here we describe the responses of hemocytes in adultDrosophilato oxidative stress and the essential role of non-canonical DNA damage repair activity in direct “responder” hemocytes to control JNK-mediated stress signaling, systemic levels of the cytokine upd3 and subsequently susceptibility to oxidative stress. Our results point to an essential systemic role of hemocytes in controlling systemic oxidative stress response inDrosophila, including energy mobilization for potential tissue repair.

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Evaluation of the Effects of Imazalil on Genotoxicity and Behavioral Toxicity in Drosophila melanogaster

Özkara,

Özdemir,

Çilek

et al. 2024

Afyon Kocatepe University Journal of Sciences and Engineering

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Imazalil (IMZ) is an imidazole and triazole derivative fungicide that is widely used to prevent many diseases in vegetable and fruit fields and to prevent post-harvest spoilage. In this study, the genotoxic potential of IMZ at different concentrations (0.25, 1, 4.5 mM) on Drosophila melanogaster was investigated using Somatic Mutation and Recombination (SMART) and Single Cell Gel Electrophoresis (Comet) Assays. The effect of the same IMZ concentrations on behavioral toxicity in D. melanogaster was investigated. Larval weight, crawling, and pupa formation success were performed to determine behavioral toxicity. As a result of the study, it was determined that IMZ generally caused a negative effect on D. melanogaster. In the SMART test, it was found that the differences between the wing preparations of the individuals obtained as a result of all IMZ concentration applications were not statistically significant compared to the negative control. The damage caused to DNA by IMZ was determined by the Comet test, and a statistically significant increase in DNA damage scores was observed at doses of 1 and 4.5 mM. In the crawling experiment of IMZ on D. melanogaster, a decrease in locomotion occurred due to the increase in dose compared to the control group, and these changes were found to be statistically significant at all application doses. Changes in larval weight were not found to be statistically significant. In the pupa formation success experiment, the decrease at 1 and 4.5 mM doses was found to be statistically significant.

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Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-seq

Cited by 5 publications

References 17 publications

DNA damage signaling in Drosophila macrophages modulates systemic cytokine levels in response to oxidative stress

DNA damage signaling in Drosophila macrophages modulates systemic cytokine levels in response to oxidative stress

DNA damage signaling inDrosophilamacrophages modulates systemic cytokine levels in response to oxidative stress

Evaluation of the Effects of Imazalil on Genotoxicity and Behavioral Toxicity in Drosophila melanogaster

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