Hemocyte migration and expression of four Sox genes during wound healing in Pacific abalone, Haliotis discus hannai

Furukawa, Fumiya; Doshimo, Yumi; Sodeyama, Gin; Adachi, Kai; Mori, Kohsuke; Mori, Yuichi; Inadama, Ryota; Koyama, Mugen; Funayama, Shinji; Oda, Takuji; Furukawa, Suehiro; Moriyama, Shunsuke; Kimura, Satoshi; Kaneko, Toyoji; Okumura, Seiichi

doi:10.1016/j.fsi.2021.07.011

Cited by 9 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sox4 and Sox11 were found to be highly expressed in embryonic epidermal cells and re-expressed in postnatal skin wounds, where they re-activated an embryonic signature, including cytoskeleton genes, facilitating cell migration 30 . In molluscs, a marine gastropod, SoxC was suggested to play critical roles in wound repair; haemocytes stained with uorescent dye were found to migrate to wounds, suggesting cell migration of cells expressing four Sox genes, including SoxC to the wound sites 49 . Considering the molecular function of mammalian and molluscous SOXC in development and wound healing, we speculate that soxC-expressing cells are progenitors and that SoxC transcription factors may regulate cytoskeleton genes facilitating cell migration during blastema formation in E. japonensis.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of soxC-expressing cells facilitated by MMPreg is essential for blastema formation in annelids

Fujita

Aoki

Mori

et al. 2022

Preprint

View full text Add to dashboard Cite

Regeneration in many organisms involves the formation of a blastema that differentiates and organises into appropriate missing tissues. Although the mechanisms involved in blastema formation have been an intriguing topic of study in regeneration biology, the cellular and molecular basis of blastema formation remains poorly understood. Here, we demonstrate that during regeneration in E. japonensis, which can regenerate all organs from small fragments, the Sry-related high-mobility group (HMG) box (Sox) C-expressing cells accumulate in the head blastema. In addition, using RNAi silencing, we showed that MMPreg, a member of the matrix metalloprotease (MMP) family involved in extracellular matrix remodelling, is necessary for the accumulation of soxC-expressing cells in the head blastema, suggesting that MMPreg facilitated soxC-expressing cell migration to the head blastema. These findings provide insight into the cellular and molecular mechanisms underlying blastema formation.

show abstract

Section: Discussionmentioning

confidence: 99%

Accumulation of soxC-expressing cells facilitated by MMPreg is essential for blastema formation in annelids

Fujita

Aoki

Mori

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Fibroblast-like cells have been observed in both mollusk and echinoderm appendage regeneration ( Ben Khadra et al, 2015 ; Furukawa et al, 2021 ), but the direct contribution of these cells to regenerating tissue remains unexamined. Indeed, there seems to be relatively little comparative analysis of the fibroblast cell type across major animal taxa.…”

Section: Using Gene Regulatory Network Analyses and Tissue Dependenci...mentioning

confidence: 99%

The salamander blastema within the broader context of metazoan regeneration

Tajer

Savage

Whited

2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Throughout the animal kingdom regenerative ability varies greatly from species to species, and even tissue to tissue within the same organism. The sheer diversity of structures and mechanisms renders a thorough comparison of molecular processes truly daunting. Are “blastemas” found in organisms as distantly related as planarians and axolotls derived from the same ancestral process, or did they arise convergently and independently? Is a mouse digit tip blastema orthologous to a salamander limb blastema? In other fields, the thorough characterization of a reference model has greatly facilitated these comparisons. For example, the amphibian Spemann-Mangold organizer has served as an amazingly useful comparative template within the field of developmental biology, allowing researchers to draw analogies between distantly related species, and developmental processes which are superficially quite different. The salamander limb blastema may serve as the best starting point for a comparative analysis of regeneration, as it has been characterized by over 200 years of research and is supported by a growing arsenal of molecular tools. The anatomical and evolutionary closeness of the salamander and human limb also add value from a translational and therapeutic standpoint. Tracing the evolutionary origins of the salamander blastema, and its relatedness to other regenerative processes throughout the animal kingdom, will both enhance our basic biological understanding of regeneration and inform our selection of regenerative model systems.

show abstract

“…First, the development cycle is different at the time of sampling for female gonads of L. fortunei. Second, the shallow water environment is not stable, and the maternal immunity may be influential, given that the SOX family genes are important genes that cause differential expression patterns in female individuals of L. fortunei, and the SOX family genes are reported to be related to immunity (Furukawa et al, 2021). The ovary is an important source of maternal immunity, therefore the differential expression between individuals may be caused by their different responses to the changing environment (Wang et al, 2015).…”

Section: Putative Mechanisms Of Sex Determination or Differentiation ...mentioning

confidence: 99%

Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons

et al. 2022

View full text Add to dashboard Cite

Gigantidas platifrons is one of the most dominant deep-sea mussels in cold seeps ecosystems in the South China sea. Studies have shown that deep-sea mussels are a gonochoristic species, however, little is known about the molecular mechanisms of sex determination in G. platifrons. In this study, RNA-seq and WGBS methylation analysis were performed on adult G. platifrons gonads to identify potential sex-related genes and generate a comprehensive analysis of sex determination in deep-sea mussels. A total of 5923 genes were identified as differentially expressed between the ovaries and testes, of which 2711 were female-biased and 3212 were male-biased. Among them, 161 genes may participate in the sex determination, and we found that DMRT2 may play an important role in male sex determination, and FOXL2, Wnt7, and β-catenin may have impact on female sex determination. Moreover, common expression patterns were found in majority of the sex-related genes such as FOXL2, β-catenin, and genes in SOX family, suggesting the sex determination mechanisms of mussels in different habitats were conserved. The 5mC levels of transcription start sites (TSS2K) were significantly higher in sex-related genes than other DEGs in both gonads. Positive correlation was observed between sex-related genes expression and methylation in male, however, the effects of the DNA methylation on gene expression were complex in female. In short, we argue that sex determination mechanisms of deep-sea mussel G. platifrons is mainly controlled by genetic, and the methylation may have a regulation role on male sex determination or differentiation.

show abstract

Hemocyte migration and expression of four Sox genes during wound healing in Pacific abalone, Haliotis discus hannai

Cited by 9 publications

References 39 publications

Accumulation of soxC-expressing cells facilitated by MMPreg is essential for blastema formation in annelids

Accumulation of soxC-expressing cells facilitated by MMPreg is essential for blastema formation in annelids

The salamander blastema within the broader context of metazoan regeneration

Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons

Contact Info

Product

Resources

About