Hemocyanins from Helix and Rapana Snails Exhibit in Vitro Antitumor Effects in Human Colorectal Adenocarcinoma

Georgieva, A. I.; Todorovа, Katerina; Илиев, Иван; Dilcheva, V.; Vladov, Ivelin; Petkova, S.; Toshkova, Reneta; Velkova, Lyudmila; Dolashki, Aleksandar; Dolashka, Pavlina

doi:10.3390/biomedicines8070194

Cited by 13 publications

(20 citation statements)

References 34 publications

(50 reference statements)

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“…Especially methylated galactoses and xylose seem to be the key players of this effect [36,40,41]. In vitro studies on hemocyanins from the marine snail Rapana venosa and the garden snails Helix aspersa and Helix lucorum revealed antitumor effects on human colorectal adenocarcinoma cells [42]. A recent study shows that hemocyanins from Concholepas concholepas and Fissurella latimarginata are safe and useful carriers of carbohydrate mimotopes and [32,33], (c) HtH1 of Haliotis tuberculata [34], (d) Biomphalaria glabrata [35], (e) Helix pomatia [25,36].…”

Section: Hemolymph Proteinsmentioning

confidence: 99%

Mollusc N-glycosylation: Structures, Functions and Perspectives

Staudacher

2021

Biomolecules

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Molluscs display a sophisticated N-glycan pattern on their proteins, which is, in terms of involved structural features, even more diverse than that of vertebrates. This review summarises the current knowledge of mollusc N-glycan structures, with a focus on the functional aspects of the corresponding glycoproteins. Furthermore, the potential of mollusc-derived biomolecules for medical applications is addressed, emphasising the importance of mollusc research.

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Section: Hemolymph Proteinsmentioning

confidence: 99%

Mollusc N-glycosylation: Structures, Functions and Perspectives

Staudacher

2021

Biomolecules

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“…The hemolymph was gathered from the collected H. lucorum after cutting the foot muscles. The crude hemolymph was filtrated and centrifuged at 10 000 rpm and 4°C for 20 min to remove of rough particles and hemocytes as described by Velkova et al, (2010) and Georgieva et al (2020). Three fractions were separated from the purified hemolymph: MW<100 kDa fraction, MW<1 kDa fraction and fraction above 100 kDa (hemocyanin) by ultrafiltration (using Millipore™ Ultrafiltration Membrane Filters from 100 kDa and 1 kDa).…”

Section: Isolation Of the Bioactive Compoundsmentioning

confidence: 99%

Antioxidant activity of fractions isolated from hemolymph of garden snail Helix lucorum

Alexandrova¹,

Petrov²,

Dolashki³

et al. 2021

J Pharm Pharmacogn Res

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Context: The snail hemolymph is a multi-component mixture comprising various substances with pharmacological activity. Aims: To evaluate the antioxidant activity of hemocyanin and fractions isolated of hemolymph from garden snail Helix lucorum. Methods: The antioxidant activity was tested in chemical systems generating superoxide radicals (O2•–) and hydroxyl radicals (•OH) and was presented as percent inhibition. The fractions’ composition was analyzed by MALDI-TOF/TOF mass spectrometry. Results: At concentration of 90 µg/mL the hemolymph fraction with compounds with molecular weight (MW) <100kDa demonstrated the strongest O2•–– scavenger effect (82%), followed by the hemocyanin (58%), and hemolymph fraction with compounds with MW<1kDa (15%). The most powerful •OH scavenger effect at concentration of 90 µg/mL showed hemocyanin (43%) followed by MW<100kDa fraction (26%). No •OH scavenger effect was observed by the MW<1kDa fraction. The highest chelation properties (55%) were demonstrated by the MW<100kDa fraction at concentration of 130 µg/mL; both the hemocyanin and MW<1kDa hemolymph fraction showed 33% inhibitory effect at the same concentration. The analysis of the composition of the tested fractions revealed high content of hydrophobic surfaces in MW<1kDa fraction and presence of antioxidant enzymes-catalase, superoxide dismutase and glutathione peroxidase in MW<100kDa fraction that contribute to the overall antioxidant effect of H. lucorum hemolymph. Conclusions: The complex antioxidant effect of hemolymph from H. lucorum is thought to be related to the presence of low molecular weight peptides in the MW<1 kDa fraction including glutathione and mainly to the antioxidant enzymes in the MW<100 kDa fraction.

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“…Another approach is to look for new compounds isolated from different natural sources, such as plants and invertebrates (land- and water-dwelling); this approach has been of great interest for anticancer research. Some bioactive compounds isolated from the hemolymph of mollusc species exhibit antitumor activity, have antioxidant and immunomodulatory properties, and attract significant interest as good drug candidates for therapeutic applications [ 7 , 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Antitumor Activity of Bioactive Compounds from Rapana venosa against Human Breast Cell Lines

et al. 2023

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This study is the first report describing the promising antitumor activity of biologically active compounds isolated from the hemolymph of marine snail Rapana venosa—a fraction with Mw between 50 and 100 kDa and two structural subunits (RvH1 and RvH2), tested on a panel of human breast cell lines—six lines of different molecular subtypes of breast cancer MDA-MB-231, MDA-MB-468, BT-474, BT-549, SK-BR-3, and MCF-7 and the non-cancerous MCF-10A. The fraction with Mw 50–100 kDa (HRv 50–100) showed good antitumor activity manifested by a significant decrease in cell viability, altered morphology, autophagy, and p53 activation in treated cancer cells. An apparent synergistic effect was observed for the combination of HRv 50–100 with cis-platin for all tested cell lines. The combination of HRv 50–100 with cisplatin and/or tamoxifen is three times more effective compared to treatment with classical chemotherapeutics alone. The main proteins in the active fraction, with Mw at ~50 kDa, ~65 kDa, ~100 kDa, were identified by MALDI-MS, MS/MS analyses, and bioinformatics. Homology was established with known proteins with antitumor potential detected in different mollusc species: peroxidase-like protein, glycoproteins Aplysianin A, L-amino acid oxidase (LAAO), and the functional unit with Mw 50 kDa of RvH. Our study reveals new perspectives for application of HRv 50–100 as an antitumor agent used alone or as a booster in combination with different chemotherapies.

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Hemocyanins from Helix and Rapana Snails Exhibit in Vitro Antitumor Effects in Human Colorectal Adenocarcinoma

Cited by 13 publications

References 34 publications

Mollusc N-glycosylation: Structures, Functions and Perspectives

Mollusc N-glycosylation: Structures, Functions and Perspectives

Antioxidant activity of fractions isolated from hemolymph of garden snail Helix lucorum

Antitumor Activity of Bioactive Compounds from Rapana venosa against Human Breast Cell Lines

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