Hemoconcentration Induced By Exercise. Revisiting The Dill &amp; Costill Equation

Alis, Rafael; Risso-Ballester, Jennifer; Sanchís-Gomar, Fabián; Primo-Carrau, Cristina; Lozano-Calve, Sheyla; Dipalo, Mariella; Aloe, Rosalia; Blesa, J. Gasent; Romagnoli, Marco; Lippi, Giuseppe

doi:10.1249/01.mss.0000479102.51301.c0

Cited by 8 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, hemoconcentration, potentially resulting from dehydration, may cause similar abnormalities in an ABP. In this case, instead of increasing the volume of circulating plasma, dehydration causes a decrease in this volume, which may lead to artificially elevated values of the volume‐dependent variables [Hb] and HCT . The extent to which dehydration‐induced fluid shifts may increase [Hb], HCT, and calculated OFF‐score, especially in the context of the ABP, however, is not currently known.…”

Section: Introductionmentioning

confidence: 99%

“…Recent work has shown that the inclusion of up to eight serum biomarkers in a biological passport model may control for a portion of PV; however, limitations may exist in cost and reliability of measurement of this number of analytes . One such possible individual marker of fluid balance or PV, especially in the context of hemoconcentration, is albumin (ALB) . ALB accounts for approximately 50% of total plasma protein, is responsible for the majority of plasma oncotic pressure, and is viewed as a stable circulating protein with a normal physiologic range between 3.5 and 5.0 g/dL.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 One such possible individual marker of fluid balance or PV, especially in the context of hemoconcentration, is albumin (ALB). 17 ALB accounts for approximately 50% of total plasma protein, is responsible for the majority of plasma oncotic pressure, 20 and is viewed as a stable circulating protein with a normal physiologic range between 3.5 and 5.0 g/dL. Though shifts in ALB concentration can occur, these can often be explained physiologically.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessing serum albumin concentration following exercise‐induced fluid shifts in the context of the athlete biological passport

Miller

Teramoto

Smeal

et al. 2019

Drug Testing and Analysis

View full text Add to dashboard Cite

Purpose The hydration status of an athlete at the time of a doping control sample collection is an important factor to consider when reviewing athlete biological passports (ABPs). Dehydration results in a reduction of the circulating plasma volume (PV), which may lead to artificially high values of some blood parameters. This study aimed to identify whether serum albumin could serve as a single marker of fluid shifts, which are not currently accounted for in the hematological passport. An additional marker could be used to assist experts when interpreting irregularities in the ABP. Methods Twelve subjects underwent multiple controlled exercise trials designed to induce varying levels of PV shifts. Pre‐exercise blood samples were collected to establish baseline values for individual passports. During exercise interventions, blood samples were collected before the start of exercise and at 10 minute, 1 hour, 2 hours, and 24 hours following exercise. Results Significant increases in hematological parameters – hemoglobin [Hb], hematocrit (HCT), albumin (ALB), and calculated OFF‐score – were identified at varying time points following fluid shift‐inducing exercise. Changes in ALB correlated strongly with changes in [Hb] (r = 0.753) and with estimated PV shifts (r = −0.764). In analyzing ABPs, the resulting increases in Hb did not trigger any atypical findings at 99% specificity. Perspective Monitoring changes in ALB longitudinally may assist experts when reviewing PV shifts in the biological passport.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessing serum albumin concentration following exercise‐induced fluid shifts in the context of the athlete biological passport

Miller

Teramoto

Smeal

et al. 2019

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…For these biomarkers, reported usually as an amount in liter of a plasma or serum, customary way to make the plasma volume change correction is, for example, from (Alis et al. ),

{PM}_{post, c} = {PM}_{normalpost, u} \times (1 + Δ PV),

where PM post, c and PM post, u indicate corrected and uncorrected serum or plasma biomarker after the exercise, respectively. Another class of biomarkers from bloodstream are the whole blood biomarkers (BM).…”

Section: Resultsmentioning

confidence: 99%

“…Most biomarkers are measured from plasma or serum, such as branched chain amino acids. For these biomarkers, reported usually as an amount in liter of a plasma or serum, customary way to make the plasma volume change correction is, for example, from (Alis et al 2015),…”

Section: Resultsmentioning

confidence: 99%

Corrected whole blood biomarkers - the equation of Dill and Costill revisited

2018

View full text Add to dashboard Cite

An exercise bout or a dehydration often causes a reduction in plasma volume, which should be acknowledged when considering the change in biomarkers before and after the plasma changing event. The classic equation from Dill and Costill (1974, J. Appl. Physiol., 37, 247–248) for plasma volume shift is usually utilized in such a case. Although this works well with plasma and serum biomarkers, we argue in this note that this traditional approach gives misleading results in the context of whole blood biomarkers, such as lactate, white cells, and thrombocytes. In this study, we demonstrate that to calculate the change in the total amount of circulating whole blood biomarker, one should utilize a formula BMpostBMpre×HnormalbnormalpreHnormalbnormalpost−1. Here Hb and BM are, respectively, the concentrations for the hemoglobin and for the inspected whole blood biomarker before (pre) and after (post) the plasma changing incident.

show abstract

The use of biomarkers to describe plasma‐, red cell‐, and blood volume from a simple blood test

Lobigs

Sottas²,

Bourdon

et al. 2016

American J Hematol

View full text Add to dashboard Cite

Plasma volume and red cell mass are key health markers used to monitor numerous disease states, such as heart failure, kidney disease, or sepsis. Nevertheless, there is currently no practically applicable method to easily measure absolute plasma or red cell volumes in a clinical setting. Here, a novel marker for plasma volume and red cell mass was developed through analysis of the observed variability caused by plasma volume shifts in common biochemical measures, selected based on their propensity to present with low variations over time. Once a month for 6 months, serum and whole blood samples were collected from 33 active males. Concurrently, the CO-rebreathing method was applied to determine target levels of hemoglobin mass (HbM) and blood volumes. The variability of 18 common chemistry markers and 27 Full Blood Count variables was investigated and matched to the observed plasma volume variation. After the removal of between-subject variations using a Bayesian model, multivariate analysis identified two sets of 8 and 15 biomarkers explaining 68% and 69% of plasma volume variance, respectively. The final multiparametric model contains a weighting function to allow for isolated abnormalities in single biomarkers. This proof-of-concept investigation describes a novel approach to estimate absolute vascular volumes, with a simple blood test. Despite the physiological instability of critically ill patients, it is hypothesized the model, with its multiparametric approach and weighting function, maintains the capacity to describe vascular volumes. This model has potential to transform volume management in clinical settings.

show abstract

Hemoconcentration Induced By Exercise. Revisiting The Dill & Costill Equation

Cited by 8 publications

References 0 publications

Assessing serum albumin concentration following exercise‐induced fluid shifts in the context of the athlete biological passport

Assessing serum albumin concentration following exercise‐induced fluid shifts in the context of the athlete biological passport

Corrected whole blood biomarkers - the equation of Dill and Costill revisited

The use of biomarkers to describe plasma‐, red cell‐, and blood volume from a simple blood test

Contact Info

Product

Resources

About