Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

Link, Antonia; Cattaneo, Giorgio; Brynda, Eduard; Riedel, Tomáš; Kučerová, Jarmila; Schlensak, Christian; Wendel, Hans Peter; Krajewski, Stefanie; Michel, Tatjana

doi:10.3791/60610

Cited by 4 publications

(6 citation statements)

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“…In contact with heparinized blood in a Chandler loop or a ow loop model mimicking human blood ow, the coatings suppressed thrombin generation, platelets and leukocyte activation, complement activation and blood clot formation. 29,40 The results presented herein indicate that the binding of GFs preserve the excellent hemocompatibility of the Fb/Hep coating.…”

Section: Hemocompatibility Of the Coatingsmentioning

confidence: 62%

“…We have previously reported on the significantly improved in vitro hemocompatibility of PVC substrates 29 and neurovascular stents 40 that had been modified with Fb/Hep coatings by a similar technique as applied here. In contact with heparinized blood in a Chandler loop or a flow loop model mimicking human blood flow, the coatings suppressed thrombin generation, platelets and leukocyte activation, complement activation and blood clot formation.…”

Section: Resultsmentioning

confidence: 99%

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Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors

Táborská

Riedelová-Reicheltová

Brynda

et al. 2021

RSC Adv.

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Section: Hemocompatibility Of the Coatingsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors

Táborská

Riedelová-Reicheltová

Brynda

et al. 2021

RSC Adv.

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“…This reduction in platelet surface coverage was far greater than the amount of reduction observed for recent heparin drug-eluting stents [ 23 , 24 , 26 ], which showed far more platelet adhesion to their modified steel samples as compared to MEG-OH-coated samples, and poorer reduction in adhesion of less than 80% compared to the greater-than-90% adhesion observed for MEG-OH. Additionally, the amount of platelet reduction was greater than that observed for recent graphene surface coatings on steel which display an 80–90% reduction [ 30 , 31 , 32 ], and performed similarly to hydrogel coatings, with a greater-than-90% reduction [ 33 , 34 ].…”

Section: Resultsmentioning

confidence: 68%

“…More recent advances in drug eluting stents include heparin-eluting stents [ 22 , 23 , 24 , 25 ] and combined heparin–nitric-oxide-eluting stents [ 26 , 27 ], which elute the anti-coagulant drug heparin and anti-bacterial nitric oxide. Several different methods are used to measure the amount of platelet adhesion and thrombus formation to these coatings, but these only typically reduce thrombus formation to a level in the range of 60–80%.…”

Section: Introductionmentioning

confidence: 99%

Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel

2021

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Implantable devices fabricated from austenitic type 316L stainless steel have been employed significantly in medicine, principally because the material displays excellent mechanical characteristics and corrosion resistance. It is well known, however, that interaction of exposure of such a material to blood can initiate platelet adhesion and blood coagulation, leading to a harmful medical condition. In order to prevent undesirable surface platelet adhesion on biomaterials employed in procedures such as renal dialysis, we developed an ultrathin anti-thrombogenic covalently attached monolayer based on monoethylene glycol silane chemistry. This functions by forming an interstitial hydration layer which displays restricted mobility in the prevention of surface fouling. In the present work, the promising anti-thrombogenic properties of this film are examined with respect to platelet aggregation on 316L austenitic stainless steel exposed to whole human blood. Prior to exposure with blood, all major surface modification steps were examined by X-ray photoelectron spectroscopic analysis and surface free-angle measurement by contact angle goniometry. End-stage anti-thrombogenicity detection after 20 min of blood exposure at 100 s−1, 300 s−1, 600 s−1, 750 s−1, and 900 s−1 shear rates revealed that a significant reduction (>90%) of platelet adhesion and aggregation was achieved for surface-modified steel, compared with untreated material. This result is confirmed by experiments conducted in real time for 60-minute exposure to blood at 100 s−1, 600 s−1, and 900 s−1 shear rates.

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“…In the flow loop model blood is perfused through a heparinized tubing containing the test specimen by using a peristaltic pump at a specific rate as previously described extensively [40]. First, heparin-coated (Ension, Pittsburgh, USA) PVC tubes with an inner diameter of 3.175 mm (Saint-Gobain Performance Plastics, Courbevoie, France) and a length of 75 cm were loaded with a test specimen each.…”

Section: Blood Circulation Modelsmentioning

confidence: 99%

In vitro investigation of an intracranial flow diverter with a fibrin-based, hemostasis mimicking, nanocoating

Link¹,

Michel

Schaller

et al. 2020

Biomed. Mater.

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Flow diversion aims at treatment of intracranial aneurysms via vessel remodeling mechanisms, avoiding the implantation of foreign materials into the aneurysm sack. However, complex implantation procedure, high metal surface and hemodynamic disturbance still pose a risk for thromboembolic complications in the clinical praxis. A novel fibrin and heparin based nano coating considered as a hemocompatible scaffold for neointimal formation was investigated regarding thrombogenicity and endothelialization. The fibrin-heparin coating was compared to a bare metal as well as fibrin- or heparin-coated flow diverters. The implants were tested separately in regard to inflammation and coagulation markers in two different in vitro hemocompatibility models conducted with human whole blood (n = 5). Endothelialization was investigated through a novel dynamic in vitro cell seeding model containing primary human cells with subsequent viability assay. It was demonstrated that platelet loss and platelet activation triggered by presence of a bare metal stent could be significantly reduced by applying the fibrin-heparin, fibrin and heparin coating. Viability of endothelial cells after proliferation was similar in fibrin-heparin compared to bare metal implants, with a slight, non-significant improvement observed in the fibrin-heparin group. The results suggest that the presented nanocoating has the potential to reduce thromboembolic complications in a clinical setting. Though the new model allowed for endothelial cell proliferation under flow conditions, a higher number of samples is required to assess a possible effect of the coating.

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Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

Cited by 4 publications

References 0 publications

Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors

Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors

Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel

In vitro investigation of an intracranial flow diverter with a fibrin-based, hemostasis mimicking, nanocoating

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