Hemmung der Virushämagglutination durch virusinfizierte und nichtinfizierte Mäuselunge

Bienz, Kurt; Lazàry, S.; Loeffler, H.

doi:10.1159/000161625

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1971

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“…The purification scheme for isolation of the lipoprotein inhibitor was devised by utilizing typical biochemical fractionation procedures and noting the improvement in HAI activity on a protein basis. Bicarbonate solutions are often used in the initial stages of cell membrane preparation (4,23,26,34). Laucikova (20,21) used bicarbonate extraction and isoelectric precipitation at pH 5.1 to separate an inhibitor of influenza virus HA from chick embryo chorioallantoic membrane extracts.…”

Section: Discussionmentioning

confidence: 99%

“…Investigation of the myxovirus inhibitor in mouse lung has indicated that it is easily extracted by low molarity bicarbonate solutions (4,23). No detailed chemical characterization of this solubilized inhibitor has yet been attempted; however, it was partially purified by differential centrifugation and Sephadex G-200 filtration, indicating its macromolecular nature.…”

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confidence: 99%

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