Hemin-mediated alleviation of zinc, lead and chromium toxicity is associated with elevated photosynthesis, antioxidative capacity; suppressed metal uptake and oxidative stress in rice seedlings

Chen, Qin; Zhang, Xiaoyan; Liu, Yuanyuan; Wei, Junyu; Shen, Wenbiao; Shen, Zhenguo; Chen, Jin

doi:10.1007/s10725-016-0202-y

Cited by 103 publications

(32 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Photosynthesis is a highly integrated and regulated process, and the response of photosynthesis to environmental changes is inhibited by heavy metals such as Pb [47,48]. Chen et al [49] reported that in rice, the most basic and apparent symptoms of Pb-induced toxicity include leaf chlorosis; stunting; reduced net photosynthesis, stomatal conductance, and leaf transpiration; and less accumulation of photosynthetic pigments such as chlorophyll (Chl) a, Chl b and carotenoids. Furthermore, by maintaining the balance of photosynthetic energy, increases in chlorophyll content and the number of chloroplasts may enhance plant tolerance to Pb [16,26].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Fagopyrum tataricum leaves in response to lead stress

et al. 2020

View full text Add to dashboard Cite

Background: Lead (Pb) pollution is a widespread environmental problem that is harmful to living organisms. Tartary buckwheat (Fagopyrum tataricum), a member of the family Polygonaceae, exhibits short growth cycles and abundant biomass production, could be an ideal plant for phytoremediation due to its high Pb tolerance. Here, we aimed to explore the molecular basis underlying the responses of this plant to Pb stress. Results: In our study, ultrastructural localization assays revealed that Pb ions primarily accumulate in leaf vacuoles. RNA deep sequencing (RNA-Seq) of tartary buckwheat leaves was performed on two Pb-treated samples, named Pb1 (2000 mg/kg Pb (NO 3) 2) and Pb2 (10,000 mg/kg Pb (NO 3) 2), and a control (CK). A total of 88,977 assembled unigenes with 125,203,555 bases were obtained. In total, 2400 up-regulated and 3413 down-regulated differentially expressed genes (DEGs) were identified between CK and Pb1, and 2948 up-regulated DEGs and 3834 downregulated DEGs were generated between CK and Pb2, respectively. Gene Ontology (GO) and pathway enrichment analyses showed that these DEGs were primarily associated with 'cell wall', 'binding', 'transport', and 'lipid and energy' metabolism. The results of quantitative real-time PCR (qRT-PCR) analyses of 15 randomly selected candidate DEGs and 6 regulated genes were consistent with the results of the transcriptome analysis. Heterologous expression assays in the yeast strain Δycf1 indicated that overexpressing CCCH-type zinc finger protein 14 (ZFP14) enhanced sensitivity to Pb 2+ , while 5 other genes, namely, metal transporter protein C2 (MTPC2), phytochelatin synthetase-like family protein (PCSL), vacuolar cation/proton exchanger 1a (VCE1a), natural resistance-associated macrophage protein 3 (Nramp3), and phytochelatin synthetase (PCS), enhanced the Pb tolerance of the mutant strain. Conclusion: Combining our findings with those of previous studies, we generated a schematic model that shows the metabolic processes of tartary buckwheat under Pb stress. This study provides important data for further genomic analyses of the biological and molecular mechanisms of Pb tolerance and accumulation in tartary buckwheat.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Fagopyrum tataricum leaves in response to lead stress

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Superoxide dismutase, a metalloenzyme present in different cell compartments, is considered to be the first line of defense against oxidative stress. It dismutases O 2to O 2 and then to H 2 O 2 that is further reduced to H 2 O by the activities of CAT and GPX and maintain superoxide radicals at steady state levels (63,15). Catalase detoxifies H 2 O 2 directly in the cell.…”

Section: Discussionmentioning

confidence: 99%

Defense manifestations of enzymatic and non-enzymatic antioxidants in Ricinus communis L. exposed to lead in hydroponics

Kiran

Prasad

2019

The EuroBiotech Journal

View full text Add to dashboard Cite

Lead (Pb) is a major inorganic pollutant with no biological significance and has been a global concern. Phytotoxicity of lead induces toxic effects by generating reactive oxygen species (ROS), which inhibits most of the cellular processes in plants. Hydro-ponic experiments were performed with Ricinus communis to investigate the toxicity and antioxidant responses by exposing to different concentrations of lead (0, 200 and 400 µM) for 10 days. Pb stress caused a significant increase in electrolyte leakage, non-enzymatic antioxidants (phenols and flavonoids) and a decrease in the elemental profile of the plant. Histochemical visualization clearly indicates the significant increase of H2O2 production in dose-dependent manner under Pb stress. Likewise, an increase in catalase, guaiacol peroxidase and superoxide dismutase activity was also evident. Ascorbate peroxidase and MDAR, on the other hand, responded biphasically to Pb treatments showing a decrease in concentration. The decline in redox ratio GSH/GSSG was imposed by the indirect oxidative stress of Pb. Hence these findings showed the ameliorative potential of R. communis to sustain Pb toxicity under oxidative stress.

show abstract

“…Lead (Pb) is a non-essential element which cause phytotoxicity on plants for long time ago, and its adverse effects on morphology, seed germination, seedling growth, photosynthesis, water content, mineral nutrition, and enzymatic activities are confirmed for all plant species (12,19,(26)(27)(28)(29)(30)(31). As a general rule, the effects are more noticeable at high concentrations of Pb and duration of exposure.…”

Section: Lead Effects On Plantsmentioning

confidence: 99%

“…Generally, Zn is not considered to be highly phytotoxic metal, but contents of Zn found in surrounding contaminated soils frequently exceed the required as nutrients and may cause phytotoxicity. The main signs of Zn toxicity in various plant species (which is also relevance for Cd phytotoxicity) are indicated by decrease in growth (both roots and shoots) and development of the plant, alteration in metabolism processes and induction of oxidative damage (18,31,(42)(43)(44)(45). Zinc toxicity also causes chlorosis, at beginning of exposure in the younger leaves, and after prolonged exposure, chlorosis is extended to older leaves also (46).…”

Section: Zinc Effects On Plantsmentioning

confidence: 99%

Heavy metals and their general toxicity for plants

Ackova

2018

Plant Sci. Today

View full text Add to dashboard Cite

Heavy metals are important environmental pollutants, and their toxicity is a serious problem of great concern for environmental, ecological, nutritional and toxicological reasons. Metals can affected long list of physiological and biochemical processes in plants and their toxicity varies with plant species, particular metal, metal concentration and it chemical form. Throughout the world, researches have been conducted extensive investigations to determine the effects of toxic heavy metals on plants. The process is still going on and the need of intensification of the research programmes for better understanding of heavy metal toxicity is evident.

show abstract

Hemin-mediated alleviation of zinc, lead and chromium toxicity is associated with elevated photosynthesis, antioxidative capacity; suppressed metal uptake and oxidative stress in rice seedlings

Cited by 103 publications

References 67 publications

Transcriptome profiling of Fagopyrum tataricum leaves in response to lead stress

Transcriptome profiling of Fagopyrum tataricum leaves in response to lead stress

Defense manifestations of enzymatic and non-enzymatic antioxidants in Ricinus communis L. exposed to lead in hydroponics

Heavy metals and their general toxicity for plants

Contact Info

Product

Resources

About