Hemidesmosome formation by embryonic chick corneal epithelium in vitro

Shellswell, G. B.; Mattey, Derek L.; Garrod, David R.

doi:10.1016/0040-8166(86)90068-6

Cited by 4 publications

(1 citation statement)

References 7 publications

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“…Collagen I gelled to form a three‐dimensional collagen matrix was found to support superior levels of cell morphology and differentiation 11, 12. The formation of epithelial adhesive structures has been directly addressed by several investigators using similar collagen gel preparations 13–16. In each case, hemidesmosomes and/or basal lamina were reported at the epithelial‐collagen interface.…”

Section: Introductionmentioning

confidence: 99%

The architecture of a collagen coating on a synthetic polymer influences epithelial adhesion

Evans

McFarland

Taylor

et al. 2001

J. Biomed. Mater. Res.

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The current study sought to identify a collagen coating methodology for application to polymer surfaces that would provide for the development of adhesive structures responsible for the sustained adhesion of corneal epithelial tissue. We compared an uncoated microporous polycarbonate surface and equivalent surfaces coated with either covalently immobilized collagen I or chemically crosslinked collagen I gel in a corneal explant outgrowth assay over 21 days. Electron microscopy was used to examine the formation of hemidesmosomes, basal lamina, and anchoring fibrils at the tissue-polymer interface. The crosslinked collagen gel preparation supported the overlying epithelial tissue across the pore openings and allowed for the formation of identifiable basal lamina, hemidesmosomes, and anchoring fibrils between the epithelial tissue and the polymer surface. Hemidesmosomal plaque, but no basal lamina or anchoring fibril formation, occurred on the uncoated surface or on that coated with covalently immobilized collagen I. We propose that the collagen matrix provided by the crosslinked collagen gel was reorganized by the epithelial tissue and that this, combined with the secretion of ECM molecules, served to limit the diffusion of basement membrane components, which permitted an increase in the local concentration of these molecules, which favored the assembly of epithelial adhesive structures.

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Section: Introductionmentioning

confidence: 99%

The architecture of a collagen coating on a synthetic polymer influences epithelial adhesion

Evans

McFarland

Taylor

et al. 2001

J. Biomed. Mater. Res.

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Hemidesmosomes and anchoring fibril collagen appear synchronously during development and wound healing

Gipson

Spurr-Michaud

Tisdale

1988

Developmental Biology

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Formation of hemidesmosomes in vitro by a transformed rat bladder cell line.

Riddelle

Green

Jones

1991

The Journal of Cell Biology

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Abstract. Two hemidesmosomal plaque components of 230 and 180 kD have recently been characterized using autoantibodies in the serum samples of bullous pemphigoid (BP) patients (Klatte, D. H., M. A. Kurpakus, K. A. Grelling, and J. C. R. Jones. 1989, J. Cell Biol. 109:3377-3390). These BP autoantibodies generate the type of staining patterns that one would predict for formed hemidesmosomes, i.e., a punctate staining pattern towards the substratum; in <50% of various primary epithelial and transformed epidermal cell lines even when such cells are maintained in culture for prolonged periods. In contrast, affinitypurified human autoantibodies against the 230-kD hemidesmosomal plaque component generate intense immunofluorescence staining along the region of cell-substratum interaction in the rat bladder tumor cell line 804G maintained on uncoated glass coverslips. This pattern is distinct from that observed in the 804G cells using an antibody preparation directed against vinculin, a component of adhesion plaques. Ultrastructural analyses of the 804G cells reveals that hemidesmosome-like structures occur along the basal surface of cells where they abut the substratum. These structures are present in 804G cells maintained in culture in reduced levels of Ca 2÷ and are recognized by autoantibodies directed against the 230-kD hemidesmosomal plaque component as determined by immunogold ultrastructural localization.To study hemidesmosome appearance in this cell line, 804G ceils were trypsinized and then allowed to readhere to glass coverslips. In rounded, unattached 804G cells, hemidesmosome-like plaque structures occur along the cell surface. These structures are recognized by the 230-kD autoantibodies. At 1 h after plating, hemidesmosomes are observed along the substratum attached surface of cells. Protein synthesis is not required for the appearance of these hemidesmosomes. Within 4 h of plating, autoantibody staining and hemidesmosomes appear towards the cell periphery. Subsequently, the polypeptide recognized by the BP autoantibodies becomes concentrated in the perinuclear region, where there are numerous hemidesmosomes. We propose that the hemidesmosomes in 804G cells are involved in cell-substratum adhesion. We discuss possible mechanisms of assembly of hemidesmosomes in the 804G cells. Indeed, the 804G cells should prove an invaluable cell line for the biochemical and molecular dissection of hemidesmosome structure, function, and assembly.

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Hemidesmosome formation by embryonic chick corneal epithelium in vitro

Cited by 4 publications

References 7 publications

The architecture of a collagen coating on a synthetic polymer influences epithelial adhesion

The architecture of a collagen coating on a synthetic polymer influences epithelial adhesion

Hemidesmosomes and anchoring fibril collagen appear synchronously during development and wound healing

Formation of hemidesmosomes in vitro by a transformed rat bladder cell line.

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