“…We aimed to perform fertilization and embryotoxicity tests with three sea urchin species, having low records in appearing in ecotoxicity studies -Clypeaster japonicus, Pseudocentrotus depressus and Hemicentrotous pulcherrimus. All of them can be found in waters along the Japanese archipelago, with some having very restricted habitats, appearing in low densities and low population sizes (Agatsuma, 2013), making their populations more vulnerable to toxic chemicals.…”