2021
DOI: 10.21203/rs.3.rs-543976/v1
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Hemicentin-1 is An Essential Extracellular Matrix Component of the Dermal-Epidermal and Myotendinous Junctions

Abstract: The extracellular matrix (ECM) architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (>600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has… Show more

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Cited by 6 publications
(15 citation statements)
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References 29 publications
(67 reference statements)
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“…(U-X) Transmission electron micrographs of dermal-epidermal junctions in dorsal median fin fold of wild-type, nid2a morphant, hmcn1 tq102 or lama5 tc17 mutant embryo, 30 hpf, transverse section through tail region; inset show low magnification overviews of dorsal halves of sections, also illustrating the increased width of the dermal space between the two adjacent epidermal sheets of the dorsal median fin fold in nid2a morphant and hmcn1 and lama5 mutants. Arrows point to BM of normal morphology, empty arrow heads in R,T point to regions with indistinct BM in nid2a morphant and lama5 mutant, and filled arrowhead in S points to regions with widened BM in hmcn1 mutant, consistent with the recently reported BM defects in Hmcn1 mutant mice (49).…”
Section: Resultssupporting
confidence: 86%
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“…(U-X) Transmission electron micrographs of dermal-epidermal junctions in dorsal median fin fold of wild-type, nid2a morphant, hmcn1 tq102 or lama5 tc17 mutant embryo, 30 hpf, transverse section through tail region; inset show low magnification overviews of dorsal halves of sections, also illustrating the increased width of the dermal space between the two adjacent epidermal sheets of the dorsal median fin fold in nid2a morphant and hmcn1 and lama5 mutants. Arrows point to BM of normal morphology, empty arrow heads in R,T point to regions with indistinct BM in nid2a morphant and lama5 mutant, and filled arrowhead in S points to regions with widened BM in hmcn1 mutant, consistent with the recently reported BM defects in Hmcn1 mutant mice (49).…”
Section: Resultssupporting
confidence: 86%
“…To address whether hemicentins and nidogens interact functionally in vivo , we carried out comparative and combined loss-of-function studies. Mouse Hmcn1 mutants only show very mild defects in dermal-epidermal and mytendinous junctions (49), while defects do not seem further enhanced upon combined loss of Hmcn1 and Hmcn2 (48). This largely dispensable nature of mouse hemicentins precludes comparative and combined hemicentin-nidogen loss of function studies, and we therefore turned to zebrafish.…”
Section: Resultsmentioning
confidence: 99%
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“…The highest correlations observed for non-collagenous proteins was for THSD4 ( r = 0.95), which already correlated moderately with hydroxyproline content ( r = 0.52), and Hemicentin-1 (HMCN1) ( r = 0.92), a little-studied protein of the fibulin family (Fibulin-6) with roles in epithelial organization. 45–47…”
Section: Resultsmentioning
confidence: 99%