Hemicellulosic ethanol production by immobilized cells of<i>Scheffersomyces stipitis</i>: Effect of cell concentration and stirring

Milessi, Thais S.; Antunes, Felipe Antônio Fernandes; Chandel, Anuj K.; Silva, Sílvio Silvério da

doi:10.4161/21655979.2014.983403

Cited by 12 publications

(10 citation statements)

References 28 publications

(22 reference statements)

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“…Immobilization provides enhanced ethanol productivity in many ways. These include reduction of contamination; [35][36][37] easy separation of cell mass from the liquid medium; 36 enhanced stability of ethanol production; 38 reduced production costs; 39,40 decreased fermentation times; 34 providing cells for several cycles of fermentation; 41 and providing good barriers against inhibitors. 38 Disadvantages of immobilization may include gel degradation, significant substrate and product mass transfer limitation, and gel particle distruption due to CO 2 production and leakage of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Effective ethanol production from whey powder through immobilizedE. coliexpressingVitreoscillahemoglobin

2016

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Ethanol production from whey powder was investigated by using free as well as alginate immobilized E. coli and E. coli expressing Vitreoscilla hemoglobin (VHb) in both shake flask and fermenter cultures. Media with varying levels of whey (lactose contents of 3%, 5%, 8% or 15%) and yeast extract (0.3% or 0.5%) were evaluated with fermentation times of 48-96 h. Immobilization and VHb expression resulted in higher ethanol production with all media; the increases ranged from 2% to 89% for immobilization and from 2% to 182% for VHb expression. It was determined that growth medium containing 8% lactose with 0.5% yeast extract yielded the highest ethanol production for free or immobilized strains, with or without VHb expression, in both shake flask and fermenter cultures. Immobilization with alginate was found to be a promising process for ethanol production by VHb-expressing ethanologenic E. coli.

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Section: Introductionmentioning

confidence: 99%

Effective ethanol production from whey powder through immobilizedE. coliexpressingVitreoscillahemoglobin

2016

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“…Acid hydrolysis was conducted at 121 °C for 20 min using a solid/liquid ratio of 1:10 for dry sugar cane bagasse/acid solution. 20 The hydrolysate was separated from solid material by filtration. The recovered hydrolysate was then concentrated by a vacuum evaporator under reduced pressure at 70 °C in a 32 L capacity concentrator.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The concentrated hydrolysate was detoxified (over liming and activated charcoal combination) according to the method of Alves et al 22 The average composition of the detoxified SBHH was 31 g•L −1 xylose, 0.3 g•L −1 glucose, and 1.6 g•L −1 of acetic acid. 20 The fermentation medium was prepared by supplementing the sugar cane bagasse hydrolysate with 5.0 g•L −1 (NH 4 ) 2 SO 4 , 3 g•L −1 of yeast extract, and 3 g•L −1 of malt extract. 19 Inoculum Preparation and Cell Immobilization.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Hemicellulosic Ethanol Production in Fluidized Bed Reactor from Sugar Cane Bagasse Hydrolysate: Interplay among Carrier Concentration and Aeration Rate

Antunes

Chandel

Santos

et al. 2017

ACS Sustainable Chem. Eng.

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Development of an efficient consolidated process is pivotal in order to design industrially viable processes for conversion of lignocellulosic biomass into second generation (2G) ethanol. Aiming to develop process consolidation, here we explored fluidized bed reactor (FBR) for 2G ethanol production from sugar cane bagasse hemicellulosic hydrolysate (SBHH) employing calcium alginate immobilized cells of Scheffersomyces shehatae UFMG-HM 52.2. A 22-full factorial design of experiments was carried out in order to evaluate the effect of aeration rate (0.027, 0.069, and 0.111 min–1) and carrier concentration (55.55, 83.33, and 111.11 g. L–1) on the ethanol yield (Y P/S) and productivity (Q P). Both process variables, when used at the highest level (aeration, 0.11 min–1; immobilized carrier concentration, 111.11 g. L–1), showed maximum ethanol production (Y P/S, 0.26 g/g and Q P 0.17 g·L–1·h–1). Results showed the potential to use this immobilized yeast in a fluidized bed reactor for ethanol production from C5 sugar solution. Repeated batch fermentations in FBR showed stable ethanol yield during 6 batches (288 h) followed by a gradual decrease. The use of immobilized cells in FBR could be conducive to the development of viable 2G ethanol production processes. To the best of our knowledge, this is the first report on 2G ethanol production from immobilized S. shehatae cells employing FBR using SBHH.

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“…As células microbianas foram imobilizadas pelo método de aprisionamento em gel de alginato de cálcio, conforme Milessi et al (2015). Em um béquer de 100 mL, 1g de Alginato de Sódio (GastronomyLab®) foi diluído em 40 mL de água destilada, previamente esterilizada em autoclave a 121°C, por 15 min.…”

Section: Imobilização De Células Microbianasunclassified

Produção de ácido lático por bactérias láticas imobilizadas em soro de leite suplementado com extrato de levedura

et al. 2018

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Neste trabalho estudou-se a produção de ácido lático em reator batelada utilizando como substrato soro de leite suplementado com diferentes concentrações de extrato de levedura Saccharomyces cerevisiae (EL), por ação de cultura mista de Lactobacillus acidophilus LA-5®, Bifidobacterium BB-12® e Streptococcus thermophilus imobilizada em esferas de alginato de cálcio, em diferentes temperaturas. Foram estudados dois fatores, concentração (CONC) e temperatura (TPR), sendo adotado os níveis de 0%, 6,5% e 13% para o fator concentração de extrato de levedura e 30°C, 34°C e 38°C para o fator temperatura. Observou-se que células imobilizadas não tiveram a taxa de produção de ácido lático afetada pelo aumento da temperatura de fermentação. Por outro lado, o aumento na concentração de suplementação do meio por EL levou ao aumento da produção de ácido lático. Foi avaliada também a viabilidade das esferas de alginato de cálcio quando reutilizadas para inocular novo meio de cultura. Após cinco ciclos de reutilização as esferas não apresentaram rachaduras e modificação no diâmetro médio. A concentração de ácido aumentou cerca de 157% entre o primeiro e o último ciclo de fermentação.

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Hemicellulosic ethanol production by immobilized cells ofScheffersomyces stipitis: Effect of cell concentration and stirring

Cited by 12 publications

References 28 publications

Effective ethanol production from whey powder through immobilizedE. coliexpressingVitreoscillahemoglobin

Effective ethanol production from whey powder through immobilizedE. coliexpressingVitreoscillahemoglobin

Hemicellulosic Ethanol Production in Fluidized Bed Reactor from Sugar Cane Bagasse Hydrolysate: Interplay among Carrier Concentration and Aeration Rate

Produção de ácido lático por bactérias láticas imobilizadas em soro de leite suplementado com extrato de levedura

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