Heme oxygenase-1 (HO-1) protects human lens epithelial cells (SRA01/04) against hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis

Ma, Tianju; Chen, Tingjun; Li, Peng; Ye, Zi; Zhai, Wei; Jia, Liang; Chen, Wenqian; Sun, Ang; Huang, Yang; Wei, Shimin; Li, Zhaohui

doi:10.1016/j.exer.2016.02.013

Cited by 48 publications

(38 citation statements)

References 29 publications

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“…H 2 O 2 was regarded as one of the most important species in ROS, due to its stability and key status in ROS‐related metabolism 33, 34. Thus, in vitro H 2 O 2 tests were gradually accepted as featured tools for the study of ROS or oxidative stress 35, 36. Here, H 2 O 2 was employed to simulate in vitro oxidative stress microenvironment and rRECs were selected as model cells due to its physiological function of retinal vascularization, which is essential for structural and functional recovery of the retina 37…”

Section: Discussionmentioning

confidence: 99%

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Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury

Mao

Wang

Wei

et al. 2018

J Cellular Molecular Medi

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Oxidative stress can cause injury in retinal endothelial cells. Carboxymethyl cellulose modified with collagen peptide (CMCC) is of a distinct antioxidant capacity and potentially a good drug carrier. In this study, the protective effects of CMCC against H2O2‐induced injury of primary retinal endothelial cells were investigated. In vitro, we demonstrated that CMCC significantly promoted viability of H2O2‐treated cells, efficiently restrained cellular reactive oxygen species (ROS) production and cell apoptosis. Then, the CMCC was employed as both drug and anti‐inflammatory drug carrier for treatment of retinal ischaemia/reperfusion (I/R) in rats. Animals were treated with CMCC or interleukin‐10‐loaded CMCC (IL‐10@CMCC), respectively. In comparisons, the IL‐10@CMCC treatment exhibited superior therapeutic effects, including better restoration of retinal structural thickness and less retinal apoptosis. Also, chemiluminescence demonstrated that transplantation of IL‐10@CMCC markedly reduced the retinal oxidative stress level compared with CMCC alone and potently recovered the activities of typical antioxidant enzymes, SOD and CAT. Therefore, it could be concluded that CMCC provides a promising platform to enhance the drug‐based therapy for I/R‐related retinal injury.

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Section: Discussionmentioning

confidence: 99%

“…33,34 Thus, in vitro H 2 O 2 tests were gradually accepted as featured tools for the study of ROS or oxidative stress. 35,36 Here, H 2 O 2 was employed to simulate in vitro oxidative stress microenvironment and F I G U R E 2 Representative TUNEL images of rRECs. The rRECs were pre-treated with or without H 2 O 2 and antioxidative gels.…”

Section: Discussionmentioning

confidence: 99%

Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury

Mao

Wang

Wei

et al. 2018

J Cellular Molecular Medi

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“…The membranes were then incubated with goat anti-mouse immunoglobulin G (IgG; ab6785) or goat anti-rabbit IgG (ab6721) It has been previously reported that the H 2 O 2 induced significant oxidative stress and apoptosis in SRA01/04 cells (Ma et al, 2016). This was also confirmed in this study, as the viability of SRA01/04 cells was significantly reduced in H 2 O 2 group when compared with the control group (p < 0.01; Figure 1a).…”

Section: Western Blotmentioning

confidence: 99%

Tanshinone IIA protects lens epithelial cells from H₂O₂‐induced injury by upregulation of lncRNA ANRIL

Wang²,

Zhang

et al. 2019

Journal Cellular Physiology

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Tanshinone IIA is a lipophilic diterpene extracted from the Salvia miltiorrhiza bunge, possessing antiapoptotic and antioxidant activities. The purpose of this study was to explore the effects of Tanshinone IIA on age‐related nuclear cataract. Human lens epithelial cell line SRA01/04 was subjected to H 2O 2 to mimic a cell model of cataract. Cell Counting Kit‐8 assay, flow cytometer, and reactive oxygen species (ROS) detection were performed to evaluate the effect of Tanshinone IIA pretreatment on SRA01/04 cells injured by H 2O 2. Besides, the real‐time quantitative polymerase chain reaction was used to assess the expression of long noncoding RNA (lncRNA) antisense noncoding RNA in the INK4 locus (ANRIL). Western blot analysis was performed to detect the expression of core proteins involved in cell survival and nuclear factor‐κB (NF‐κB) pathway. H 2O 2 significantly decreased SRA01/04 cells viability, whereas increased apoptosis and ROS generation. This phenomenon was coupled with the upregulated p53, p21, Bax, cleaved caspase‐3, and the downregulated cyclinD1, CDK4, and Bcl‐2. Tanshinone IIA pretreatment protected SRA01/04 cells against H 2O 2‐induced injury. In the meantime, the expression of lncRNA ANRIL was upregulated by Tanshinone IIA. And, the protective effects of Tanshinone IIA on H 2O 2‐stimulated SRA01/04 cells were abolished when lncRNA ANRIL was silenced. Moreover, the elevated expression of lncRNA ANRIL induced by Tanshinone IIA was abolished by BAY 11‐7082 (an inhibitor of NF‐κB). To conclude, Tanshinone IIA protects SRA01/04 cells from apoptosis triggered by H 2O 2. Tanshinone IIA confers its protective effects possibly via modulation of NF‐κB signaling and thereby elevating the expression of lncRNA ANRIL.

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“…HO-1 overexpression via gene transfer can ameliorate the retinal ischemia-reperfusion injury and the succeeding retinal ganglion cell (RGC) apoptosis. 19 Another study 20 has shown that HO-1 overexpression protects human lens epithelial cells from oxidative insults. However, the exact role of HO-1 in RP pathology remains to be explored.…”

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confidence: 99%

CoPP-Induced-Induced HO-1 Overexpression Alleviates Photoreceptor Degeneration With Rapid Dynamics: A Therapeutic Molecular Against Retinopathy

Tao

Cai

Zhou

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Retinitis pigmentosa (RP) causes progressive photoreceptor degeneration in the retina. The N-methyl-N-nitrosourea (MNU)-administered mouse is used as a chemically induced RP model with rapid progression rate. This study was designed to study heme oxygenase-1 (HO-1) expression in the MNU-administered mice, and to explore the therapeutic effects of cobalt protoporphyrin (CoPP). METHODS. The HO-1 expression in the retina of MNU-administered mice was analyzed. CoPP was injected intravenously into the MNU-administered mice. Subsequently, the CoPP-treated mice were subjected to functional and morphologic examinations. RESULTS. HO-1 was involved in the MNU-induced photoreceptor degeneration. CoPP treatment enhanced retinal HO-1 expression in the MNU-administered mice. Electroretinogram (ERG) examination and behavioral tests showed that CoPP treatment improved the retinal responsiveness of MNU-administered mice. Histologic analysis and optical coherence tomography (OCT) examination showed that retinal architecture of the CoPP-treated mice was more intact than that of the MNUþvehicle group. Cone photoreceptors in the MNUadministered mice were rescued efficiently by CoPP treatment. Furthermore, multielectrode array (MEA) recording showed that CoPP treatment mitigated the spontaneous firing response, enhanced the light-induced firing response, and preserved the basic configurations of visual signal pathway in the MNU-administered mice. Mechanism studies suggested that CoPP afforded these therapeutic effects by modulating the apoptosis cascades and alleviating the oxidative stress in degenerative retinas. CONCLUSIONS. CoPP alleviated photoreceptor degeneration and rectified the signaling abnormities in MNU-administered mice. CoPP may serve as a potential medication against degenerative retinopathy.

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Heme oxygenase-1 (HO-1) protects human lens epithelial cells (SRA01/04) against hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis

Cited by 48 publications

References 29 publications

Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury

Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury

Tanshinone IIA protects lens epithelial cells from H₂O₂‐induced injury by upregulation of lncRNA ANRIL

CoPP-Induced-Induced HO-1 Overexpression Alleviates Photoreceptor Degeneration With Rapid Dynamics: A Therapeutic Molecular Against Retinopathy

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Heme oxygenase-1 (HO-1) protects human lens epithelial cells (SRA01/04) against hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis

Cited by 48 publications

References 29 publications

Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury

Collagen peptide modified carboxymethyl cellulose as both antioxidant drug and carrier for drug delivery against retinal ischaemia/reperfusion injury

Tanshinone IIA protects lens epithelial cells from H2O2‐induced injury by upregulation of lncRNA ANRIL

CoPP-Induced-Induced HO-1 Overexpression Alleviates Photoreceptor Degeneration With Rapid Dynamics: A Therapeutic Molecular Against Retinopathy

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Tanshinone IIA protects lens epithelial cells from H₂O₂‐induced injury by upregulation of lncRNA ANRIL