Heme Oxygenase-1: A Metabolic Nike

Węgiel, Barbara; Németh, Zsuzsanna; Corrêa-Costa, Matheus; Bulmer, Andrew Cameron; Otterbein, Leo E.

doi:10.1089/ars.2013.5667

Cited by 147 publications

(120 citation statements)

References 124 publications

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“…Transfection was performed using 100 nM siRNA. Logarithmic growth phase cells were harvested and plated at a density of 2x10 5 cells/well in 6-well plates. Cells were grown and the expression of HO-1 in transfected cells quantified using real-time PCR.…”

Section: Patients and Tissue Specimens Medical Records At The Unionmentioning

confidence: 99%

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Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines

Ren

Yang

et al. 2016

Oncology Reports

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Abstract.Patients with esophageal squamous cell carcinoma (ESCC) have a poor prognosis. However, the related mechanisms are unclear, thus we investigated the expression of HO-1 in ESCC tissue and explored possible mechanisms of tumor progression. Expression of HO-1 was examined by immunohistochemistry in 143 ESCC tumors. The correlation of HO-1 with clinicopathological characteristics was also examined. Two human ESCC cell lines, TE-13 and Eca109 were studied. Silencing of cell line HO-1 by specific small interfering RNA (siRNA) was evaluated using real-time quantitative PCR. Cell line viability, apoptosis and intracellular levels of reactive oxygen species (ROS) after transfection were determined using MTT and flow cytometry, respectively. HO-1, Bax, Bcl-2 and A-caspase-3/-9 expression was evaluated using western blot analyses. We found that HO-1 was expressed in 58 of 143 ESCC tumors, mainly in the cytoplasm. There was a significant association between HO-1 expression and tumor grade (P<0.001). Knockdown of HO-1 expression in cell lines was associated with significantly decreased cellular proliferation (P<0.05) and a higher rate of apoptosis (P<0.001) 48 h after treatment. Treatment of the cell lines with the ROS inhibitor N-acetylcysteine abrogated this effect. Knockdown of HO-1 was associated with increased A-caspase-3 and -9 expression, but no change in Bax or Bcl-2 expression or Bax/Bcl-2 ratio was observed. Thus, the present study identified that ESCC tumors frequently expressed HO-1. Knockdown of HO-1 promoted apoptosis through activation of a ROS-mediated caspase apoptosis pathway.

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Section: Patients and Tissue Specimens Medical Records At The Unionmentioning

confidence: 99%

“…Cell growth is inhibited with inhibition of HO-1 and increased Nrf-2 expression (4). Increased HO-1 expression is also associated with increased removal of reactive oxygen species (ROS) and maintenance of the internal cellular environment (5).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines

Ren

Yang

et al. 2016

Oncology Reports

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“…HO-1, a metabolic enzyme utilizes electrons supplied by NADPH CYP 450 reductase (CPR) and oxygen to break the heme moiety to liberate biliverdin (BV), carbon monoxide (CO) and iron. Though heme acts as a prime part of numerous metabolic enzymes as a core catalytic unit and as a cofactor in enzymatic processes (e.g., cytochrome p450, iNOS and guanylate cyclase) [5], its accumulation during oxidative stress (OS) may cause extensive toxicity and free radical generation. Hence it is degraded by the cytoprotective enzyme HO-1, which is the only catalytic process that enables removal of toxic heme.…”

Section: Introductionmentioning

confidence: 99%

Hif1α Mediated Ho-1 Expression in Fish Adipocytes: Role of Hypoxia In Ennore Estuary

Perumal¹,

Parasuraman²

2017

J Marine Sci Res Dev

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Heme oxygenase-1 (HO-1), an inducible stress protein; is involved in the regulation of adipogenesis, cellular bioenergetics and cytoprotective mechanism against hypoxia induced oxidative stress. Polluted aquatic environment leads to large oxygen fluctuations which may cause alteration in fish adaptation mechanism by HIF1α regulation. Environmental pollutants have a high tendency to accumulate, persist and bio-concentrate in adipocytes due to its lipophilic nature which ultimately causes adipocytes hypoxia that in turn affects metabolic energy balance. CYP1A2 and HO-1 are the chief detoxifying and cytoprotective enzymes involved in the oxidative metabolism of a wide variety of pollutants and its mediated cytotoxic products. The key role of hypoxia and its mediated signaling protein changes in fish adipocytes due to low dissolved oxygen in Ennore estuary was demonstrated in our previous work. Therefore, we analyzed the effect of pollutants induced hypoxia by assessing CD, HNE, TAC, G3PDH, ATP, HIF1α, HO-1 and CYP1A2 in fish adipocytes of control/unpolluted site and test/polluted site. Increase in CD, HNE, HIF1α, HO-1 along with a decrease in TAC, G3PDH, ATP and CYP1A2 observed in test adipocytes. From the results achieved, it shows that induction of HO-1 by HIF1α is crucial in maintaining the cell integrity to secure adipocytes and its maturation in the fish collected from the hypoxic environment. This study also highlights the role of HIF1α and HO-1 in regulating the expression of CYP1A2 during pollutants induced hypoxic condition.

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“…This review presents the current knowledge of heme synthesis and trafficking in metazoans, and highlights recent advances in the regulation of these pathways. The regulation of heme degradation has been reviewed recently [15,16] and is not discussed here.…”

mentioning

confidence: 99%

Regulation of heme biosynthesis and transport in metazoa

Sun

Cheng

Chen

2015

Sci. China Life Sci.

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Heme is an iron-containing tetrapyrrole that plays a critical role in regulating a variety of biological processes including oxygen and electron transport, gas sensing, signal transduction, biological clock, and microRNA processing. Most metazoan cells synthesize heme via a conserved pathway comprised of eight enzyme-catalyzed reactions. Heme can also be acquired from food or extracellular environment. Cellular heme homeostasis is maintained through the coordinated regulation of synthesis, transport, and degradation. This review presents the current knowledge of the synthesis and transport of heme in metazoans and highlights recent advances in the regulation of these pathways. heme, iron, synthesis, transport, regulation Citation:Sun FX, Cheng YJ, Chen CY. Regulation of heme biosynthesis and transport in metazoa.

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Heme Oxygenase-1: A Metabolic Nike

Abstract: In this review, we describe the cross-talk between heme oxygenase-1 (HO-1) and its products with other metabolic pathways. HO-1, which we have labeled Nike, the goddess who personified victory, dictates triumph over pathophysiologic conditions, including diabetes, ischemia, and cancer.

Cited by 147 publications

References 124 publications

Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines

Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines

Hif1α Mediated Ho-1 Expression in Fish Adipocytes: Role of Hypoxia In Ennore Estuary

Regulation of heme biosynthesis and transport in metazoa

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