Hematuria Due to Adenoviral Infection in Bone Marrow Transplant Recipients

Bil‐Lula, Iwona; Ussowicz, Marek; Rybka, Blanka; Wendycz-Domalewska, Danuta; Ryczan, R; Gorczyńska, Ewa; Kałwak, Krzysztof; Woźniak, Mieczysław

doi:10.1016/j.transproceed.2010.08.049

Cited by 28 publications

(40 citation statements)

References 18 publications

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“…In addition to the promptness of detection, determination of the viral load is valuable because it may have predictive value for disseminated adenoviral disease (Claas et al 2005;Lion et al 2003). In viral hemorrhagic cystitis with concomitant signs of disseminated infection, real-time PCR technique may also benefit in discrimination between infections caused by adenoviruses, polyomavirus BK and herpes simplex viruses, with regard to different treatment strategies used in infections with these viruses (Bil-Lula et al 2010a). Infections caused by adenoviruses should be also included in the differential diagnosis of a wide variety of clinical syndromes, including pneumonia, enteritis, hemorrhagic cystitis, conjunctivitis, and disseminated disease.…”

Section: Discussionmentioning

confidence: 99%

“…Polymerase chain reaction (PCR), as well as its variant, real-time PCR (qPCR), has become a valuable tool for the detection of adenoviral DNA due to its speed, specificity, and sensitivity which allow for early diagnosis (Bil-Lula et al 2010a;Rola et al 2007). Quantitative or semiquantitative PCR assays allow also monitoring of the kinetics of the viral infection or effectiveness of antiviral treatment (Claas et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Disseminated Adenovirus Disease in Immunocompromised Patient Successfully Treated with Oral Ribavirin: A Case Report

Ulrych

Dzieciątkowski

Przybylski

et al. 2011

Arch. Immunol. Ther. Exp.

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In patients with immunological disorders, adenovirus infections are associated with significant rates of morbidity and mortality. Only few hematological units use molecular virological methods, such as polymerase chain reaction, for surveillance of adenovirus infection, and treatment strategies have never been evaluated in multicenter clinical trials. This report describes the detection and treatment of human adenovirus (HAdVs) disseminated disease in the case of a 46-year-old immunocompromised female having myelodysplastic syndrome with refractory cytopenia with multilineage dysplasia: International Prognostic Scoring System 1. Serum and urine samples were tested for the presence of adenoviral DNA using the quantitative real-time polymerase chain reaction (PCR) assay. For additional confirmation, sequencing of PCR products was also performed. With real-time PCR, we detected HAdV DNA in both serum and urine samples. The viral level constantly decreased with applied oral ribavirin therapy. As the result of sequencing, HAdVs type 11 was determined. Surveillance of adenovirus by real-time PCR is useful in detecting and monitoring disseminated HAdV infection; it is a potential standard diagnostic approach that could assist clinicians to decide whether antiviral therapy ought to be administered.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Disseminated Adenovirus Disease in Immunocompromised Patient Successfully Treated with Oral Ribavirin: A Case Report

Ulrych

Dzieciątkowski

Przybylski

et al. 2011

Arch. Immunol. Ther. Exp.

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“…A screening examination of all clinical samples was performed with singleplex qualitative PCR assays, described previously [9]. Briefly, assays consist of HotStarTaq Master Mix Kit (QIAagen GmbH, Germany), forward and reverse primers (0.5 µM final conc.…”

Section: Methodsmentioning

confidence: 99%

“…Although HAdV infections in immunocompetent subjects are usually asymptomatic and self-limiting, the recipients of HSCTs and other immunocompromised patients present a broad spectrum of clinical manifestations. They can cause a range of infections from asymptomatic to localized or disseminated [3], including acute respiratory disease [7, 8], keratoconjunctivitis [8], pharyngeal–conjunctival fever [1] and hemorrhagic cystitis [7, 9]. Less frequent complications are arthritis, encephalitis [10, 11], gastroenteritis [12, 13] and hepatitis [7, 14].…”

Section: Introductionmentioning

confidence: 99%

Co-infection with human polyomavirus BK enhances gene expression and replication of human adenovirus

Bil‐Lula

Woźniak

2018

Arch Virol

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Immunocompromised patients are susceptible to multiple viral infections. Relevant interactions between co-infecting viruses might result from viral regulatory genes which trans-activate or repress the expression of host cell genes as well as the genes of any co-infecting virus. The aim of the current study was to show that the replication of human adenovirus 5 is enhanced by co-infection with BK polyomavirus and is associated with increased expression of proteins including early region 4 open reading frame 1 and both the large tumor antigen and small tumor antigen. Clinical samples of whole blood and urine from 156 hematopoietic stem cell transplant recipients were tested. We also inoculated adenocarcinomic human alveolar basal epithelial cells with both human adenovirus 5 and BK polyomavirus to evaluate if co-infection of viruses affected their replication. Data showed that adenovirus load was significantly higher in the plasma (mean 7.5 x 103 ± 8.5 x 102 copies/ml) and urine (mean 1.9 x 103 ± 8.0 x 102 copies/ml) of samples from patients with co-infections, in comparison to samples from patients with isolated adenovirus infection. In vitro co-infection led to an increased (8.6 times) expression of the adenovirus early region 4 open reading frame gene 48 hours post-inoculation. The expression of the early region 4 open reading frame gene positively correlated with the expression of BK polyomavirus large tumor antigen (r = 0.90, p < 0.0001) and small tumor antigen (r = 0.83, p < 0.001) genes. The enhanced expression of the early region 4 open reading frame gene due to co-infection with BK polyomavirus was associated with enhanced adenovirus, but not BK polyomavirus, replication. The current study provides evidence that co-infection of adenovirus and BK polyomavirus contributes to enhanced adenovirus replication. Data obtained from this study may have significant importance in the clinical setting.

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“…45 Patients with adenovirus nephritis present with fever, gross hematuria and AKI; most have detectable adenovirus in their urine. Adenovirus cystitis is a more frequent but less morbid complication of adenovirus infection that has been witnessed in up to 15% of HCT recipients 46 and usually manifests within the first 2 months following the transplant. Isolated case reports 47,48 of successful treatment of adenovirus cystitis with IV or intravesical administration of cidofovir have been described; however, supportive care is usually the recommended treatment.…”

Section: Pathogenesismentioning

confidence: 99%

Acute kidney injury in HCT: an update

Lopes

Jorge

Neves

2016

Bone Marrow Transplant

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Acute kidney injury (AKI) is highly prevalent whether the patients undergo myeloablative or non-myeloablative hematopoietic cell transplantation (HCT); however, the pathogenesis and risk factors leading to AKI can differ between the two. The prognosis of AKI in patients receiving HCT is poor. In fact, AKI following HCT is associated not only with increased short-and long-term mortality, but also with progression to chronic kidney disease. Herein, the authors provide a comprehensive and up-to-date review of the definition and diagnosis, as well as of the incidence, pathogenesis and outcome of AKI in patients undergoing HCT, centering on the differences between myeloablative and non-myeloablative regimens. INTRODUCTIONHematopoietic cell transplantation (HCT) is currently used to treat numerous malignant (for example, multiple myeloma, leukemias and lymphomas) and non-malignant hematological disorders (for example, aplastic anemia, b-thalassemia, immunodeficiency disorders and inborn errors of metabolism), as well as solid tumors (for example, breast cancer and neuroblastoma), which are in other instances incurable.The two major HCT procedures, taking into consideration the conditioning regimen used, are myeloablative autologous and allogeneic HCT and non-myeloablative allogeneic HCT. On the one hand, myeloablative HCT utilizes the maximally tolerated dose of TBI with or without chemotherapy, or with chemotherapy alone. On the other hand, non-myeloablative HCT depends more on donor cellular immune effects and less on the cytotoxic effects of the preparative regimen to control the underlying disease. 1,2 It ultimately uses a lower dose conditioning regimen and can thus be offered to older patients, to those debilitated by additional comorbidities, or to high-risk, heavily pretreated patients, who would not tolerate myeloablative HCT, resulting in a consequent decrease in regimen-related toxicity and treatment-related mortality. [3][4][5] Acute kidney injury (AKI) is highly prevalent whether the patients undergo myeloablative or non-myeloablative regimens; however, the pathogenesis and risk factors leading to AKI can differ between the two. In fact, AKI in patients receiving HCT is associated not only with increased short-and long-term mortality as compared with patients with no AKI, but also with a higher rate of progression to chronic kidney disease (CKD). Herein, the authors provide a comprehensive and up-to-date review of the definition and diagnosis of AKI as well as of the incidence, risk factors, pathogenesis and outcome of AKI in patients undergoing HCT, centering on the differences between myeloablative and nonmyeloablative regimens.

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Hematuria Due to Adenoviral Infection in Bone Marrow Transplant Recipients

Cited by 28 publications

References 18 publications

Disseminated Adenovirus Disease in Immunocompromised Patient Successfully Treated with Oral Ribavirin: A Case Report

Disseminated Adenovirus Disease in Immunocompromised Patient Successfully Treated with Oral Ribavirin: A Case Report

Co-infection with human polyomavirus BK enhances gene expression and replication of human adenovirus

Acute kidney injury in HCT: an update

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