“…Different implementations of multi-frequency ultrasound are also summarized, including single-element transducer, double transducers, triple transducers and phased array transducers, each with its own specialty and favored for particular tasks. Benefited from lesion size control, cavitation enhancement, low power needs and multi-foci treatment, this technique offers a wide range of applications, including: sonothrombolysis, tissue (tumor) ablation, sonodynamic treatment, sonochemical treatment, temperature rise, cell differentiation, BBB opening, transdermal drug delivery, sub-harmonic imaging enhancement and split-focus treatment [2] , [6] , [7] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [22] , [23] , [25] , [28] , [30] , [32] , [33] , [36] , [50] , [51] , [53] , [54] , [55] , [60] , [68] , [69] , [70] , [71] , [72] , [73] , [74] , [75] , [76] , [77] , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [23] , [75] . Moreover, this review discusses about the decisive parameters for multi-frequency treatment and suggests guidelines for parameter selection.…”