Hematopoietic stem cell stretches and moves in its bone marrow niche

Silva, Walison N.; Costa, Alinne C.; Picoli, Caroline C.; Rocha, Beatriz G S; Santos, Gabryella S P; Costa, P.A.C.R.; Azimnasab-Sorkhabi, Parviz; Soltani-Asl, Maryam; Silva, Rodrigo A. da; Amorim, Jaime Henrique; Resende, Rodrigo R.; Mintz, Akiva; Birbrair, Alexander

doi:10.1016/j.critrevonc.2021.103368

Cited by 7 publications

(2 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hematopoietic microenvironment consists of bone marrow stromal cells, HSCs, cytokines, cell metabolites and extracellular matrix (Silva et al. 2021 ). The hematopoietic microenvironment is important for hematopoietic function recovery and HSCs regeneration (Liu et al.…”

Section: Discussionmentioning

confidence: 99%

Danggui Buxue decoction alleviates cyclophosphamide-induced myelosuppression by regulating β-hydroxybutyric acid metabolism and suppressing oxidative stress

Gao

Zhang

Liu

et al. 2023

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Danggui Buxue Decoction (DBD) is an effective complementary medicine in alleviating myelosuppression after chemotherapy (MAC). However, its mechanism of action is elusive. Objective To illustrate that regulating β-hydroxybutyric acid (β-OHB) metabolism and suppressing oxidative stress could be a potential mechanism of action for DBD in alleviating MAC. Materials and methods After HPLC quantification and dose testing (3, 6 and 10 g/kg, gavage) of DBD, Sprague-Dawley rats were divided into control, cyclophosphamide (CTX) (30 mg/kg CTX for 5 days, intraperitoneal administration) and CTX + DBD groups (6 g/kg DBD for 14 days, gavage). Blood cell counts, thigh bone histological examination, β-OHB levels, oxidative stress indices and HDAC1 activity were tested. The biological function of β-OHB was verified in vitro (hBMSC cells were incubated in culture mediums that contained 40 μM CTX and β-OHB in 0, 1, 2.5, 5, 10 mM) and in vivo (MAC rat model, 3 g/kg β-OHB for 14 days, gavage). Results Rats in the CTX + DBD group showed upregulated blood cell counts (118–243%), β-OHB levels (495 nmol/mL in blood, 122 nmol/mg in marrow supernatant) and downregulated HDAC1 activity (59%), and oxidative stress indices (60–85%). In vitro , 5 mM β-OHB improved hBMSC cell migration (123%) and proliferation (131%). In vivo , rats treated with 3 g/kg β-OHB showed upregulated blood cell counts (121–182%) and downregulated HDAC1 activity (64%) and oxidative stress indices (65–83%). Discussion and conclusions DBD, a traditional Chinese medicine, alleviates MAC by intervening in β-OHB metabolism and oxidative stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Danggui Buxue decoction alleviates cyclophosphamide-induced myelosuppression by regulating β-hydroxybutyric acid metabolism and suppressing oxidative stress

Gao

Zhang

Liu

et al. 2023

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…Hematopoietic stem cells in the bone marrow clonally proliferate, leading to leukaemia, a kind of blood cancer (Akinduro et al, 2018;Roy et al, 2022;Silva et al, 2021). Leukemia new cases were reported at a rate of 14.1 per 100,000 adult adults annually.…”

Section: Introductionmentioning

confidence: 99%

Selective binding and induction of death in leukemia cells by Biodegradable magnetized iron nanodiscs

Kharat

Pottathil

2023

Preprint

View full text Add to dashboard Cite

Leukemia is a blood malignancy with a rate of new cases of 14.1 per 100,000 adults per year. Leukemic cells seek a way to escape from the cytotoxic effects of chemotherapy drugs, leading to treatment failure. In this paper, we demonstrated the separation of circulating leukaemia cells using an antibody-conjugated iron oxide nanodisc (MND-Ab) under a low magnetic field gradient. Biodegradable magnetic nanodiscs were prepared by using iron oxide, BSA and the antibody IPO-M6, which is an anti-HL-60 antibody. Atomic force microscope studies show that the nano-discs were sprayed uniformly with an average size of 22+/-2.1 nm. The efficiency of cell capture was monitored with Prussian blue staining and fluorescence microscopy. A bar magnet was placed near the plate at 2 mm distance from the centre of the plate. The labelled cells were gathered near the magnet after 2 minutes. When the MND-Ab-HL60 cells were exposed to magnetic oscillations (5 min) 10 htz, 90 Guass), 92–94% of the cells were found apoptotic or dead in treated HL60 cells compared to the untreated control.

show abstract

Basics of Stem Cell Transplant

Sharma¹

2023

Basics of Hematopoietic Stem Cell Transplant

View full text Add to dashboard Cite

Hematopoietic stem cell stretches and moves in its bone marrow niche

Cited by 7 publications

References 87 publications

Danggui Buxue decoction alleviates cyclophosphamide-induced myelosuppression by regulating β-hydroxybutyric acid metabolism and suppressing oxidative stress

Danggui Buxue decoction alleviates cyclophosphamide-induced myelosuppression by regulating β-hydroxybutyric acid metabolism and suppressing oxidative stress

Selective binding and induction of death in leukemia cells by Biodegradable magnetized iron nanodiscs

Basics of Stem Cell Transplant

Contact Info

Product

Resources

About