Hematopoietic Recovery after Transplantation CD117+B220- (lacZ+) Bone Marrow Cells in Lethally Irradiated Mice

Hodek, Miroslav; Vávrová, Jiřina; Šinkorová, Zuzana; Mokrý, Jaroslav; Filip, Stanislav

doi:10.14712/18059694.2017.6

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…In our earlier studies, we monitored their plasticity, migration and reparative potential . In particular, transplanted bone marrow cells represent a heterogeneous system, and their plasticity allows the regeneration and repair of damaged tissues and the maintenance of homeostasis in the organism .…”

Section: Discussionmentioning

confidence: 99%

The peripheral chimerism of bone marrow–derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice

Filip

Mokrý

Vávrová

et al. 2014

J Cellular Molecular Medi

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Bone marrow–derived cells represent a heterogeneous cell population containing haematopoietic stem and progenitor cells. These cells have been identified as potential candidates for use in cell therapy for the regeneration of damaged tissues caused by trauma, degenerative diseases, ischaemia and inflammation or cancer treatment. In our study, we examined a model using whole-body irradiation and the transplantation of bone marrow (BM) or haematopoietic stem cells (HSCs) to study the repair of haematopoiesis, extramedullary haematopoiesis and the migration of green fluorescent protein (GFP+) transplanted cells into non-haematopoietic tissues. We investigated the repair of damage to the BM, peripheral blood, spleen and thymus and assessed the ability of this treatment to induce the entry of BM cells or GFP+lin−Sca-1+ cells into non-haematopoietic tissues. The transplantation of BM cells or GFP+lin−Sca-1+ cells from GFP transgenic mice successfully repopulated haematopoiesis and the haematopoietic niche in haematopoietic tissues, specifically the BM, spleen and thymus. The transplanted GFP+ cells also entered the gastrointestinal tract (GIT) following whole-body irradiation. Our results demonstrate that whole-body irradiation does not significantly alter the integrity of tissues such as those in the small intestine and liver. Whole-body irradiation also induced myeloablation and chimerism in tissues, and induced the entry of transplanted cells into the small intestine and liver. This result demonstrates that grafted BM cells or GFP+lin−Sca-1+ cells are not transient in the GIT. Thus, these transplanted cells could be used for the long-term treatment of various pathologies or as a one-time treatment option if myeloablation-induced chimerism alone is not sufficient to induce the entry of transplanted cells into non-haematopoietic tissues.

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Section: Discussionmentioning

confidence: 99%

The peripheral chimerism of bone marrow–derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice

Filip

Mokrý

Vávrová

et al. 2014

J Cellular Molecular Medi

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“…After bone marrow reconstitution, NK cells in these mice express the human diphtheria toxin receptor (DTR) and can be selectively depleted by injection of diphtheria toxin (DT). Of note, higher percentages of TER119 + cells were already present in mock-infected chimeras irrespective of DT treatment, most likely due to a higher background level of EMH during the reconstitution phase of the hematopoietic system following bone marrow transfer (Hodek et al, 2008) (Figure 2E). Nevertheless, NK cell depletion by DT-injection significantly decreased the number of MCMVinduced TER119 + cells in these mice.…”

Section: Nk Cells Are Essential For Extramedullary Hematopoiesis Uponmentioning

confidence: 99%

Natural Killer Cells Are Required for Extramedullary Hematopoiesis following Murine Cytomegalovirus Infection

Jordan¹,

Ruzsics²,

Mitrović³

et al. 2013

Cell Host & Microbe

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The immune response against a variety of pathogens can lead to activation of blood formation at ectopic sites, a process termed extramedullary hematopoiesis (EMH). The underlying mechanisms of EMH have been enigmatic. Investigating splenic EMH in mice infected with murine cytomegalovirus (MCMV), we find that, while cells of the adaptive immune system were dispensable for EMH, natural killer (NK) cells were essential. EMH required recognition of infected cells via activating NK cell receptors Ly49H or NKG2D, and correspondingly, viral interference with NK cell recognition abolished EMH. Surprisingly, development of EMH was not induced by NK cell-derived cytokines but was dependent on perforin-mediated cytotoxicity in order to control virus spread. Spreading virus reduced the numbers of F4/80(+) macrophages that were crucial for inflammatory EMH. Hence, whereas MCMV suppresses inflammation-induced EMH, NK cells confine virus spread, thereby protecting extramedullary hematopoietic niches and facilitating EMH.

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Colonization of recipient tissues with transplnted murine bone marrow cells

Filip¹,

Mokrý²,

Čížková³

et al. 2012

Transfusion and Apheresis Science

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Hematopoietic Recovery after Transplantation CD117+B220- (lacZ+) Bone Marrow Cells in Lethally Irradiated Mice

Cited by 4 publications

References 17 publications

The peripheral chimerism of bone marrow–derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice

The peripheral chimerism of bone marrow–derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice

Natural Killer Cells Are Required for Extramedullary Hematopoiesis following Murine Cytomegalovirus Infection

Colonization of recipient tissues with transplnted murine bone marrow cells

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