Hematopoietic reconstitution of CD34+ cells derived from short-term cultured cord blood mononuclear cells

Abstract

^Äëíê~Åí= bñ=îáîç expansion of hematopoietic stem cells (HSCs) is very important for clinical applications of cord blood (CB). With the aim to find proper culture duration for Éñ=îáîç expansion, mononuclear cells (MNC) was applied as starting culture cells to expand HSCs and the repopulating potential of seven-day and fourteen-day cultured CD34 + cells were compared. The average expansion of total cells and CD34 + cells cultured for 7 days were higher than those cultured for 14 days. The results of phenotypic analysis of fresh and cultured cells showed that the percentage of CD3 + cells declined and the percentage of CD33 + cells increased during culture. The engraftment levels of fourteen-day cultured CD34 + cells were higher than those of fresh and seven-day cultured CD34 + cells. Fourteen-day cultured CD34 + cells also showed better multilineage reconstitution ability than fresh and seven-day cultured CD34 + cells. The results of the present study demonstrated that prolonged culture could preserve the hematopoietic reconstitution ability of ex vivo cultured CB cells and improve the engraftment level in NOD/SCID mice. © KSBB hÉóïçêÇëW=ÅçêÇ=ÄäççÇI=ãçåçåìÅäÉ~ê=ÅÉääëI=`aPQ + =ÅÉääëI=ex vivo=Éñé~åëáçåI=klaLp`fa=ãáÅÉ