Hematopoietic progenitor cells as targets for non-invasive prenatal diagnosis: detection of fetal CD34+ cells and assessment of post-delivery persistence in the maternal circulation☆☆Presented in part at the 12th Fetal Cell Workshop, Prague, May 2001.

Guetta, Esther; Gordon, Daphna; Simchen, Michal J.; Goldman, Bruce; Barkai, Gad

doi:10.1016/s1079-9796(03)00008-1

Cited by 74 publications

(60 citation statements)

References 21 publications

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“…In all human pregnancies, fetal progenitor cells that express CD34 are transferred into the maternal circulation (Guetta et al, 2003); they can be isolated by culturing maternal blood during pregnancy and up to six months after delivery (Osada et al, 2001). The number of fetal progenitor cells circulating in the blood of pregnant women has been estimated to be 0-2/mL (Guetta et al, 2003).…”

Section: Fetal Stem Cells Are Transferred During Pregnancy From the Fmentioning

confidence: 99%

Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse

Khosrotehrani

Bianchi

2005

Journal of Cell Science

154

109

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Fetal cells circulate in pregnant women and persist in blood and tissue for decades post-partum. The mother thus becomes chimeric. Factors that may influence such fetal cell microchimerism include histocompatibility, fetal or placental abnormalities, or a reproductive history that includes miscarriage or elective termination. Fetal cell microchimerism is associated with some maternal autoimmune diseases, such as systemic sclerosis. Moreover, a novel population of fetal cells, the pregnancy-associated progenitor cells (PAPCs), appears to differentiate in diseased or injured maternal tissue. The cellular origin of these cells is at present unknown but could be a hematopoietic stem cell, a mesenchymal stem cell, or a novel cell type. Pregnancy therefore results in the acquisition of cells with stem-cell-like properties that may influence maternal health post-partum. Rather than triggering disease, these cells may instead combat it.

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Section: Fetal Stem Cells Are Transferred During Pregnancy From the Fmentioning

confidence: 99%

Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse

Khosrotehrani

Bianchi

2005

Journal of Cell Science

154

109

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“…A largescale study for this cell-based approach, conducted by the National Institute of Child Health and Human Development in the USA, demonstrated that detection of trisomy in these fetal nucleated erythrocyte cells is difficult possibly due to the fact that the chromosomes in these cells disintegrate some time before the nucleus is eliminated from the cell, making FISH analysis of samples from maternal circulation unreliable [Bianchi et al, 2002;Babochkina et al, 2005]. The use of fetal cells other than fetal nucleated red blood cells also found in maternal circulation has been studied, however these cells are able to persist for years, or even decades, following previous pregnancies and this persistence limits their potential value for NIPD [Guetta et al, 2003]. Possible explanations include a simple presence of these fetal cells or the fact that the maternal hematopoietic system becomes engrafted with fetal stem cells during pregnancy [Puszyk et al, 2008].…”

Section: Intact Fetal Cells and Cell-free Fetal Dna In The Maternal Cmentioning

confidence: 99%

Non Invasive Prenatal Diagnosis of Down Syndrome

Kappou¹,

Papadopoulou²,

Sifakis³

2011

Prenatal Diagnosis and Screening for Down Syndrome

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“…These engrafted cells appear to have the ability of multi-directional differentiation and express differentiation markers. For example, fetal microchimeric cells are capable of engraftment and differentiation along the hematopoietic pathway [11][12][13] . Studies from Dr. Nelson's lab [14] showed that fetal cells found in the liver of women who had given birth to sons included cells that expressed hepatocyte antigens (cytokeratins); studies by Wang et al [15] showed that, after tissue injury in the liver and kidneys, engrafted fetal cells transformed into hepatocytes and tubular cells.…”

Section: Introductionmentioning

confidence: 99%

Fetal cell microchimerism in the maternal mouse spinal cord

Zhang

Zhao

et al. 2013

Neurosci. Bull.

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Fetal cell microchimerism refers to the persistence of fetal cells in the maternal tissues following pregnancy. It has been detected in peripheral organs and the brain, but its existence in the spinal cord has not been reported. Our aim was to detect fetal cell microchimerism in the spinal cord of maternal mice. C57BL/6 female mice were crossed with GFP transgenic male mice and sacrificed after their first or third delivery. GFP-positive cells, which were presumably from fetuses whose fathers were GFP transgenic, were detected in the spinal cord by fl uorescence microscopy and immunohistochemistry. PCR was also performed to detect GFP DNA, which must come from GFP hemizygous fetuses. We found GFP-positive cells and detectable GFP DNA in most of the maternal spinal cords. Twenty percent (1/5) of the mice that were only pregnant once had detectable fetal cells, while 80% (4/5) of those that were pregnant three times had detectable fetal cells. Some fetal cells, which not only emitted green fluorescence but also expressed NeuN, were detected in the spinal cords from maternal mice. These results indicate that fetal cells migrate into the spinal cord of a maternal mouse during and/or after the gestational period, and the fetal cells may differentiate into neurons in the spinal cord.

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Hematopoietic progenitor cells as targets for non-invasive prenatal diagnosis: detection of fetal CD34+ cells and assessment of post-delivery persistence in the maternal circulation☆☆Presented in part at the 12th Fetal Cell Workshop, Prague, May 2001.

Cited by 74 publications

References 21 publications

Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse

Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse

Non Invasive Prenatal Diagnosis of Down Syndrome

Fetal cell microchimerism in the maternal mouse spinal cord

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