Hematopoietic growth factors in cancer

Moore, Malcolm A.S.

doi:10.1002/1097-0142(19900201)65:3+<836::aid-cncr2820651331>3.0.co;2-x

Cited by 15 publications

(8 citation statements)

References 32 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentrations of growth factors used in the experiments described above were based on clinical values used to treat refractory anaemias and to stimulate leukocyte recovery after cytotoxic chemotherapy (Oster et al, 1990;Moore, 1990). Clearly caution should be observed in the use of rEPO and rGM-CSF following chemotherapy and the use of rEPO in managing refractory anaemias in prostate cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Production and response of human prostate cancer cell lines to granulocyte macrophage‐colony stimulating factor

Lang¹,

Miller²,

Duncan

et al. 1994

Intl Journal of Cancer

View full text Add to dashboard Cite

Prostate cancer selectively metastasises to skeletal sites, where it normally produces osteoblastic lesions. This study investigated whether haematopoietic growth factors known to be present in the bone environment could be involved in the survival and proliferation of prostate skeletal metastases. To evaluate this hypothesis we investigated the effects of recombinant granulocyte/macrophage colony-stimulating factor (rGM-CSF), recombinant granulocyte colony-stimulating factor (rG-CSF), recombinant erythropoietin (rEPO) and recombinant interleukin-3 (rIL-3) on the growth of 3 human prostate cancer cell lines. Two hormone-insensitive cell lines, PC-3 and DU145, were significantly stimulated by rGM-CSF and rEPO in serum-free medium but their growth was unaffected by incubation with rIL-3 or rG-CSF. A hormone-sensitive cell line, LNCaP, was stimulated only by rGM-CSF. To investigate further the involvement of GM-CSF in prostate cancer, the presence of GM-CSF protein in the 3 prostate cancer cell lines was examined by immunohistochemistry, and analysis of cell line conditioned media was carried out by ELISA and Western blotting. These techniques demonstrated that GM-CSF-like material was produced by both DU145 and PC-3 cells but not by LNCaP. The results from ELISA found that media conditioned by DU145 and PC-3 cells contained 1.7 and 2.5 pg GM-CSF/micrograms protein, respectively, whereas no GM-CSF was detectable in the LNCaP conditioned media. Our results were also confirmed by Western blot analysis demonstrating one single band for DU145 and PC-3 conditioned media which co-migrated along with the standard rGM-CSF band. No bands were associated with the LNCaP conditioned media. The presence of GM-CSF gene transcripts in DU145 and PC-3 cells was established by reverse transcription and polymerase chain reaction of total RNA.

show abstract

Section: Discussionmentioning

confidence: 99%

Production and response of human prostate cancer cell lines to granulocyte macrophage‐colony stimulating factor

Lang¹,

Miller²,

Duncan

et al. 1994

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…G-CSF is the single most important factor for inducing the generation of new granulocytes from bone marrow. G-CSF is also used to treat neutropenia induced by cancer chemo or radiation therapy [4].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

Jiang

Schwarz

2010

PLoS ONE

View full text Add to dashboard Cite

BackgroundGranulocytes and monocytes/macrophages differentiate from common myeloid progenitor cells. Granulocyte colony-stimulating factor (G-CSF) and CD137 (4-1BB, TNFRSF9) are growth and differentiation factors that induce granulocyte and macrophage survival and differentiation, respectively. This study describes the influence of G-CSF and recombinant CD137-Fc protein on myelopoiesis.Methodology/Principal FindingsBoth, G-CSF and CD137 protein support proliferation and survival of murine bone marrow cells. G-CSF enhances granulocyte numbers while CD137 protein enhances macrophage numbers. Both growth factors together give rise to more cells than each factor alone. Titration of G-CSF and CD137 protein dose-dependently changes the granulocyte/macrophage ratio in bone marrow cells. Both factors individually induce proliferation of hematopoietic progenitor cells (lin-, c-kit+) and differentiation to granulocytes and macrophages, respectively. The combination of G-CSF and CD137 protein further increases proliferation, and results in a higher number of macrophages than CD137 protein alone, and a lower number of granulocytes than G-CSF alone demonstrating that CD137 protein-induced monocytic differentiation is dominant over G-CSF-induced granulocytic differentiation. CD137 protein induces monocytic differentiation even in early hematopoietic progenitor cells, the common myeloid progenitors and the granulocyte macrophage progenitors.Conclusions/SignificanceThis study confirms earlier data on the regulation of myelopoiesis by CD137 receptor - ligand interaction, and extends them by demonstrating the restriction of this growth promoting influence to the monocytic lineage.

show abstract

“…CSF activities have been shown to be produced by human melanoma, ovarian and breast cancers, and murine Lewis lung carcinoma (LLC) and mammary tumors (Ramakrishnan et al, 1989;Sabatini et al, 1990;Fu et al, 1990;Young et al, 1991b). One of these CSFs, granulocyte-macrophage-(GM-) CSF, has traditionally been considered to function in the stimulation of myelopoiesis (Cosman, 1988;Morstyn and Burgess, 1988;Moore, 1990), but has more recently been shown to also modulate various immunological functions (Fu et al, 1990;Young et al, 1990b;Bermudez and Young, 1990). In addition, GM-CSF can induce the appearance of immune suppressor cells and thus facilitate tumor dissemination (Tsuchiya et al, 1988;Fu et al, 1990Fu et al, , 1991Young et al, 1991b).…”

mentioning

confidence: 99%

Stimulation of the metastatic properties of lewis‐lung‐carcinoma cells by autologous granulocyte‐macrophage colony‐stimulating factor

Young

Lozano

Coogan

et al. 1992

Intl Journal of Cancer

View full text Add to dashboard Cite

Using both polymerase-chain-reaction analysis and the soft-agar colony-forming unit assay, granulocyte-macrophage colony-stimulating factor (GM-CSF) was shown to be expressed by cloned metastatic Lewis-lung-carcinoma (LLC-LN7) cells but not by non-metastatic LLC-C8 cells. Furthermore, the metastatic LLC-LN7 cells were shown to respond both to autologous GM-CSF and to exogenous recombinant GM-CSF (rGM-CSF). In the presence of neutralizing anti-GM-CSF antibodies, the metastatic LLC cells became less able to migrate or to adhere and invade through a reconstituted basement membrane. Moreover, the addition of rGM-CSF further enhanced the capacity of the metastatic LLC cells to adhere to the reconstituted basement membrane. This stimulation of metastatic properties of the LLC cells by either autologous or exogenous GM-CSF was associated with enhanced endogenous protein phosphorylation. Two proteins of approximately Mr 45,000 and Mr 64,000 were the dominant target proteins to be phosphorylated by the presence of GM-CSF. These results suggest that autologous GM-CSF may function as an autocrine stimulator of the metastatic properties of metastatic LLC cells.

show abstract

Hematopoietic growth factors in cancer

Cited by 15 publications

References 32 publications

Production and response of human prostate cancer cell lines to granulocyte macrophage‐colony stimulating factor

Production and response of human prostate cancer cell lines to granulocyte macrophage‐colony stimulating factor

Regulation of Granulocyte and Macrophage Populations of Murine Bone Marrow Cells by G-CSF and CD137 Protein

Stimulation of the metastatic properties of lewis‐lung‐carcinoma cells by autologous granulocyte‐macrophage colony‐stimulating factor

Contact Info

Product

Resources

About