Hematopoietic cell transformation by reticuloendotheliosis virus: Characterization of the genetic defect

Hoelzer, Jacalyn D.; Lewis, Robert B.; Wasmuth, Carl R.; Bose, Henry R.

doi:10.1016/0042-6822(80)90536-x

Cited by 87 publications

(66 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C4-1 cells (RECC-UTC4-1) are a nonvirus-producing lymphoblastoid cell line obtained from avian reticuloendotheliosis virus transformation of spleen cells from 3-week old SPAFAS chickens (Hoelzer et al, 1980). C4-1 cells are maintained as suspension cultures in RPMI-1640 medium (HyClone, Logan, UT) supplemented with 5% fetal calf serum (HyClone Laboratories), 2 mM glutamine, 100 mg/ml streptomycin, and 100 IU/ml penicillin (Sigma Chemical, St Louis, MO) at 378C in a humidi®ed atmosphere of 5% CO 2 ± 95% air.…”

Section: Cells and Virusesmentioning

confidence: 99%

c-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells

Qian

Králová

et al. 1997

Oncogene

View full text Add to dashboard Cite

Section: Cells and Virusesmentioning

confidence: 99%

c-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells

Qian

Králová

et al. 1997

Oncogene

View full text Add to dashboard Cite

“…Fresh spleen cells were prepared from 19-day-old SPAFAS (Norwich, Conn.) virus-free chickens as described previously (17,21). Cells were resuspended at approximately 3 x 107 cells per 0.4 ml of TMEM containing 20% fetal bovine serum in sterile 0.4-cm electroporation cuvettes (Bio-Rad).…”

Section: Rosenthalmentioning

confidence: 99%

A protein kinase-A recognition sequence is structurally linked to transformation by p59v-rel and cytoplasmic retention of p68c-rel.

Mosialos¹,

Hamer²,

Capobianco³

et al. 1991

Mol. Cell. Biol.

107

View full text Add to dashboard Cite

The Rel family of proteins includes a number of proteins involved in transcriptional control, such as the retroviral oncoprotein v-Rel, c-Rel, the Drosophila melanogaster developmental protein Dorsal, and subunits of the transcription factor NF-KB. These proteins are related through a highly conserved domain of approximately 300 amino acids, called the Rel homology domain, that contains dimerization, DNA binding, and nuclear targeting functions. Also within the Rel homology domain, there is a conserved consensus sequence (Arg-Arg-Pro-Ser) for phosphorylation by cyclic AMP-dependent protein kinase (PKA). We used Ilnker insertion mutagenesis and site-directed mutagenesis to determine the importance of this sequence In addition, they suggest that phosphorylation at the conserved PKA site could have a negative effect on transformation and transcriptional repression by v-Rel and induce the nuclear localization of c-Rel.

show abstract

“…REV-T causes a fatal malignancy of lymphoid cells in several species of fowl and transforms hemopoietic cells in cultures of spleen or bone marrow (6)(7)(8). Neoplastic transformation by REV-T is mediated by the oncogene v-rel, which arose by transduction of the protooncogene c-rel from the genome of a turkey (9).…”

mentioning

confidence: 99%

Rearrangements of chicken immunoglobulin genes in lymphoid cells transformed by the avian retroviral oncogene v-rel.

Chen

Lim

Bose

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The retroviral oncogene v-rel transforms poorly characterized lymphoid cells. We have explored the nature of these cells by analyzing the configuration and expression of immunoglobulin genes in chicken hemopoietic cells transformed by v-rel. None of the transformed cells expressed their immunoglobulin genes. The cells fell into three classes: class I cells have their immunoglobulin genes potentially in an embryonic configuration; class II and class III cells have lost one copy of the lambda light chain locus and have one copy of the heavy chain locus rearranged into a configuration that differs from what is found in mature B cells. In class II cells, the other heavy chain locus may be in embryonic configuration, whereas it is deleted in class III cells. The first of these classes may represent the earliest stage of the lymphoid lineage yet encountered among virus-transformed cells, whereas the second and third classes represent an apparently anomalous rearrangement whose origin remains unknown.

show abstract

Hematopoietic cell transformation by reticuloendotheliosis virus: Characterization of the genetic defect

Cited by 87 publications

References 29 publications

c-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells

c-Jun involvement in vitamin E succinate induced apoptosis of reticuloendotheliosis virus transformed avian lymphoid cells

A protein kinase-A recognition sequence is structurally linked to transformation by p59v-rel and cytoplasmic retention of p68c-rel.

Rearrangements of chicken immunoglobulin genes in lymphoid cells transformed by the avian retroviral oncogene v-rel.

Contact Info

Product

Resources

About