Hematopoetik Kök Hücre İzolasyonunda Aldh Etki̇nli̇ği̇ni̇n Önemi̇

Abstract

Objective:The preferred method for the purification of hematopoietic stem cells (HSCs), which can easily differentiate into blood cells, is the selection of CD34 + cells by flow cytometry or magnetic discrimination. Methods are needed that effectively determine human stem cells without entirely relying on phenotypic cell surface markers. One of these methods is cytosolic aldehyde dehydrogenase (ALDH), which plays a role in retinoid metabolism and resistance of HSCs to alkylating agents such as cyclophosphamide. The prospective isolation of human hematopoietic stem and progenitor cells with different functions, as well as surface markers, are also recommended by ALDH and flow cytometry. In the above findings, ALDH + cells can be isolated from other cells and the most primitive hematopoietic stem cells can be isolated and used in clinical applications. Materials and methods: In our study, we separated the CD34 surface marker cells from cord blood using magnetic nanoparticles and then purified the cells with ALDH activity and non-CD38 surface antigen by flow cytometer. We visualized these cell groups with fluorescence microscopy. Results: We observed that the majority of CD34 + cells were ALDH + in fluorescence microscopy and flow cytometry (FACS) analysis of CD34 + cells purified from cord blood as a result of ALDH-FITC markings.