Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p

Gao, Chuang; Gong, Zhongying; Wang, Dong; Huang, Jinhao; Yu, Qian; Nie, Meng; Jiang, Weiwei; Liu, Xuanhui; Luo, Hongliang; Yuan, Jiangyuan; Xiang, Tangtang; An, Shuo; Quan, Wei; Wei, Huijie; Zhang, Jianning; Jiang, Rongcai

doi:10.18632/aging.102550

Cited by 8 publications

(9 citation statements)

References 40 publications

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“…Bone marrow mesenchymal stem cell-derived exosomes can stimulate bone regeneration by regulating ossification and angiogenesis ( 57 , 58 ). Exosomes from chronic subdural hematomas promote highly permeable angiogenesis and inhibit hematoma absorption through miR144-5P ( 40 ). In the tumor microenvironment, tumor cell-derived exosomes serve as a vehicle for intercellular communication.…”

Section: Tumor Cell-derived Exosomes and Angiogenesismentioning

confidence: 99%

Exosomes and Their Role in Cancer Progression

et al. 2021

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Exosomes from extracellular vesicles can activate or inhibit various signaling pathways by transporting proteins, lipids, nucleic acids and other substances to recipient cells. In addition, exosomes are considered to be involved in the development and progression of tumors from different tissue sources in numerous ways, including remodeling of the tumor microenvironment, promoting angiogenesis, metastasis, and invasion, and regulating the immune escape of tumor cells. However, the precise molecular mechanisms by which exosomes participate in these different processes remains unclear. In this review, we describe the research progress of tumor cell-derived exosomes in cancer progression. We also discuss the prospects of the application of exosomes combined with nanoengineered chemotherapeutic drugs in the treatment of cancer.

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Section: Tumor Cell-derived Exosomes and Angiogenesismentioning

confidence: 99%

Exosomes and Their Role in Cancer Progression

et al. 2021

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“…Exosomes isolated during ICH in hemorrhagic stroke patients showed temporal profiling, and exosomes released from ECs, leukocytes, and RBCs increased. In another study, the expression of miR-144-5p was high in hematoma-derived exosomes from patients with chronic subdural hematoma in hemorrhagic stroke [ 116 ]. MiR-144-5p inhibits the expression of SDC3, which can inhibit EC migration and tube formation, thereby inhibiting angiogenesis [ 116 , 117 ].…”

Section: Diagnostic Value Of Exosomes In Strokementioning

confidence: 99%

Utility of Exosomes in Ischemic and Hemorrhagic Stroke Diagnosis and Treatment

Lee

et al. 2022

IJMS

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Stroke is the leading cause of death and neurological disorders worldwide. However, diagnostic techniques and treatments for stroke patients are still limited for certain types of stroke. Intensive research has been conducted so far to find suitable diagnostic techniques and treatments, but so far there has been no success. In recent years, various studies have drawn much attention to the clinical value of utilizing the mechanism of exosomes, low toxicity, biodegradability, and the ability to cross the blood–brain barrier. Recent studies have been reported on the use of biomarkers and protective and recovery effects of exosomes derived from stem cells or various cells in the diagnostic stage after stroke. This review focuses on publications describing changes in diagnostic biomarkers of exosomes following various strokes and processes for various potential applications as therapeutics.

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“…CSDH fluids were collected into vacuum tubes and centrifuged at 2000g for 20 min at 4 °C to collect the supernatants. Both haematoma supernatants and serum samples were aliquoted and stored at −80 °C until analyses [21]. Cells in haematoma freshly collected from CSDH were immediately analyzed using flow cytometry.…”

Section: Patients and Treatmentsmentioning

confidence: 99%

“…Cytokines, chemokines and angiogenesis-related proteins secreted by macrophages into the conditioned media were detected using human cytokine (R&D Systems, Minneapolis, USA) and angiogenesis (R&D Systems, Minneapolis, USA) array kits and quantified by pixel density using Quantity One software version 4.6.6 (Bio-Rad, USA) [21].…”

Section: Cytokine and Angiogenesis Arraysmentioning

confidence: 99%

Dexamethasone enhances the efficacy of atorvastatin in inhibiting excessively inflammation-induced abnormal angiogenesis by regulating macrophages

Gong

Zhan

Nie

et al. 2021

J Neuroinflammation

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Background We have recently showed that atorvastatin (ATO) combined with low dose of dexamethasone (DEX) was more efficacious in treating patients with chronic subdural haematoma (CSDH) than ATO monotherapy. This study was designed to investigate the underlying mechanisms of the improved efficacy of this combined therapy. Methods Mass spectrometry was performed to quantitatively detect drugs in haematoma fluids and serum samples from CSDH patients and also in cultured macrophages after treatment with either ATO alone or in combination with DEX. The differentiation and apoptosis of macrophages were evaluated using flow cytometry. The expression of cytokines, chemokines and angiogenesis-related proteins was evaluated using proteome profile arrays, immunoblots and ELISA, respectively. Results ATO was detected in haematoma fluids and serum samples, whose levels were increased significantly in samples collected from patients treated with both ATO and DEX. ATO was also increased in cultured macrophages treated with ATO and DEX. The numbers of M1-polarized macrophages were higher than the M2 phenotype in the haematoma fluids of patients. Cultured macrophages treated with ATO and DEX had reduced numbers of M1-polarized macrophages, increased numbers of M2-polarized macrophages as compared to monotherapies, and decreased rate of apoptosis induced by high-dose DEX. DEX enhanced the anti-inflammatory and anti-angiogenic activity of ATO by suppressing VEGFA and other inflammatory angiogenic factors. Consistent with the finding, patients responded well to the drug treatments had lower serum levels of VEGFA. Conclusions We have shown for the first time that ATO given orally was detected in CSDH haematoma fluids. DEX enhances the anti-inflammatory and anti-angiogenic effects of ATO, primarily by increasing the presence of ATO in haematoma and macrophages and by regulating the functions of macrophages.

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Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p

Cited by 8 publications

References 40 publications

Exosomes and Their Role in Cancer Progression

Exosomes and Their Role in Cancer Progression

Utility of Exosomes in Ischemic and Hemorrhagic Stroke Diagnosis and Treatment

Dexamethasone enhances the efficacy of atorvastatin in inhibiting excessively inflammation-induced abnormal angiogenesis by regulating macrophages

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