Hematological effects of ultrafine carbon black on red blood cells and hemoglobin

Pan, Jie; Li, Chao; Zhang, Xun; Liu, Rutao

doi:10.1002/jbt.22438

Cited by 6 publications

(4 citation statements)

References 63 publications

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“…The lowest concentration, on the other hand, does not reach 50% hemolysis at all. In accordance with our results, previous studies have reported that carbonaceous ultrafine particles have minimal hemolytic effects, indicating limited damage to the erythrocyte membrane [60].…”

Section: Discussionsupporting

confidence: 93%

“…Erythrocyte osmotic fragility test: The methodology utilized for the present study includes an accurate control for the possible presence of blood diseases that might generate false positive or false negative results. Hemolysis caused by different types of nanoparticles (polystyrene, titania, magnetite, gold, silver, silica, zincite, and selenium) has been widely reported in the literature [ 33 , 37 , 59 , 60 , 61 ]. The osmotic fragility (OF) test is commonly used to evaluate the erythrocyte membrane and diagnose hemolytic anemia.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of higher concentrations of Si in the cellular content after a short exposure is evidence of the passive Morphological changes after CNP exposure: Our morphological analysis provides further insights into the full picture; the presence of extracellular vesicles and the alteration in erythrocyte shape suggest subtle damage to the membrane. According to the biological mechanisms behind the morphological findings [69,72], oxidative stress, changes in phospholipid structure, and loss in protein anchorage to the cytoskeleton are the possible underlying mechanisms triggered by CNPs; both lipid peroxidation and oxidative injury have been observed in erythrocytes exposed to carbonaceous nanoparticles [60] (Pan et al, 2020). Another finding is the budding of echinocytes releasing vesicles under 100 nm in size.…”

Section: Morphological Analysis In Sem and Energy Dispersive X-ray Sp...mentioning

confidence: 99%

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Erythrocyte Vulnerability to Airborne Nanopollutants

Hermosillo-Abundis,

Angulo-Molina,

Méndez-Rojas

2024

Toxics

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The toxicological impact of airborne polluting ultrafine particles (UFPs, also classified as nanoparticles with average sizes of less than 100 nm) is an emerging area of research pursuing a better understanding of the health hazards they pose to humans and other organisms. Hemolytic activity is a toxicity parameter that can be assessed quickly and easily to establish part of a nanoparticle’s behavior once it reaches our circulatory system. However, it is exceedingly difficult to determine to what extent each of the nanoparticles present in the air is responsible for the detrimental effects exhibited. At the same time, current hemolytic assessment methodologies pose a series of limitations for the interpretation of results. An alternative is to synthesize nanoparticles that model selected typical types of UFPs in air pollution and evaluate their individual contributions to adverse health effects under a clinical assay of osmotic fragility. Here, we discuss evidence pointing out that the absence of hemolysis is not always a synonym for safety; exposure to model nanopollutants, even at low concentrations, is enough to increase erythrocyte susceptibility and dysfunction. A modified osmotic fragility assay in combination with a morphological inspection of the nanopollutant–erythrocyte interaction allows a richer interpretation of the exposure outcomes. Membrane–nanoparticle interplay has a leading role in the vulnerability observed. Therefore, future research in this line of work should pay special attention to the evaluation of the mechanisms that cause membrane damage.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Morphological Analysis In Sem and Energy Dispersive X-ray Sp...mentioning

confidence: 99%

See 1 more Smart Citation

Erythrocyte Vulnerability to Airborne Nanopollutants

Hermosillo-Abundis,

Angulo-Molina,

Méndez-Rojas

2024

Toxics

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“…UFCB is usually a kind of nanomaterial which is difficult to dissolve in water. Pan et al 20 use hydrogen peroxide to modify UFCB to enhance the water solubility of UFCB. Qu et al 21 use ozone to modify UFCB.…”

Section: Modification Of Carbon Blackmentioning

confidence: 99%

Study of the effects of ultrafine carbon black on the structure and function of trypsin

Huo

Zhao

et al. 2020

J of Molecular Recognition

Self Cite

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Due to the rapid development of industrial society, air pollution is becoming a serious problem which has being a huge threat to human health. Ultrafine particles (UFPs), one of the major air pollutants, are often the culprits of human diseases. At present, most of the toxicological studies of UFPs focus on their biological effects on lung cells and tissues, but there are less researches taking aim at the negative effects on functional proteins within the body. Therefore, we experimentally explored the effects of ultrafine carbon black (UFCB) on the structure and function of trypsin. After a short‐term exposure to UFCB, the trypsin aromatic amino acid microenvironment, protein backbone and secondary structure were changed significantly, and the enzyme activity showed a trend that rose at first, then dropped. In addition, UFCB interacts with trypsin in the form of a complex. These studies demonstrated the negative effects of UFCB on trypsin, evidencing potential effects on animals and humans.

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