Hematological, biochemical alterations and methane production in sheep submitted to mixed infection of Haemonchus contortus and Trichostrongylus colubriformis

Fernandes, Murilo Antônio; Lima, Paulo de Mello Tavares; Amarante, Alessandro Francisco Talamini do; Abdalla, Adibe Luiz; Louvandini, Hélder

doi:10.1016/j.smallrumres.2022.106798

Cited by 6 publications

(15 citation statements)

References 18 publications

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“…One important finding from the present study regarding enteric CH 4 emissions was that H. contortus infection did not affect the absolute daily enteric CH 4 emissions (g/d) or CH 4 yield (g/kg DMI). This is in contrast with findings from other studies (Fox et al, 2018;Lima et al, 2019;Correâ et al, 2021;Fernandes et al, 2022), which showed not only increased CH 4 yields from GIN infected sheep, but also depressed feed intake and LWG. The research by Lima et al (2019); Correâ et al (2021) and Fernandes et al (2022) were all conducted with parasite naive lambs of a tropical sheep breed (Santa Ines), fed either on Cynodon dactylon hay as a sole diet (Correâ et al, 2021) or a mixed diet containing 70% (Lima et al, 2019) or 60% (Fernandes et al, 2022) of this forage plus concentrates.…”

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...contrasting

confidence: 99%

“…This is in contrast with findings from other studies (Fox et al, 2018;Lima et al, 2019;Correâ et al, 2021;Fernandes et al, 2022), which showed not only increased CH 4 yields from GIN infected sheep, but also depressed feed intake and LWG. The research by Lima et al (2019); Correâ et al (2021) and Fernandes et al (2022) were all conducted with parasite naive lambs of a tropical sheep breed (Santa Ines), fed either on Cynodon dactylon hay as a sole diet (Correâ et al, 2021) or a mixed diet containing 70% (Lima et al, 2019) or 60% (Fernandes et al, 2022) of this forage plus concentrates. Correâ et al (2021) reported increase in CH 4 yield of 62 and 99%, respectively when the lambs were separately infected with H.contortus and T. colubriformes.…”

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...contrasting

confidence: 99%

“…For example, Rira et al (2016) reported more abundant total ruminal bacteria and methanogens in animals kept in tropical environments than in temperate environments. Nevertheless, much lower CH 4 yields were reported from other studies with sheep (Amaral et al, 2016;Lima et al, 2019;Correâ et al, 2021;Fernandes et al, 2022). For example, the study of Amaral et al (2016) reported CH 4 yield of 13 g/kg DMI from sheep fed on high quality (N fertilized) C4 annual grasses.…”

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...mentioning

confidence: 88%

“…Correâ et al (2021) reported increase in CH 4 yield of 62 and 99%, respectively when the lambs were separately infected with H.contortus and T. colubriformes. In turn, Fernandes et al (2022) reported a 59% increase in CH 4 yield from lambs infected with both H. contortus and T. colubriformes, whereas for a similar mixed infection, Lima et al (2019) reported an up to 250% increase in CH 4 yield. In a similar way, from a research work with parasite naïve lambs infected with T. circumcincta and fed on grass pellets (DM digestibility of 55.4-58.4%), Fox et al (2018) reported 33% increase in CH 4 yield.…”

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...mentioning

confidence: 94%

“…Furthermore, a study by Correâ et al (2021) reported an increase in CH 4 yield of 38% and 50%, respectively, when lambs were separately infected with 5,000 H. contortus L 3 and 10,000 Trichostrongylus (T.) colubriformes L 3 respectively. Likewise, Fernandes et al (2022) reported a 37% increase in CH 4 yield from lambs with a mixed infection of 10,000 H. contortus L 3 and 30,000 T. colubriformis L 3 , whereas for similar mixed infection, Lima et al (2019) reported up to 61% increase in CH 4 yield in lambs infected with 5,000 H. contortus and 7,500 T. colubriformis L 3 stage larvae. However, a study conducted by Houdijk et al (2017) with periparturient ewes infected with the abomasal worm Teladorsagia circumcincta, showed a 10% decrease in daily enteric CH 4 emission (g/day), with no effect on CH 4 yield (g/kg DMI).…”

Section: Introductionmentioning

confidence: 94%

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Impact of Haemonchus contortus infection on feed intake, digestion, liveweight gain, and enteric methane emission from Red Maasai and Dorper sheep

Mwangi¹,

Eckard²,

Gluecks³

et al. 2023

Front. Anim. Sci.

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A study was conducted with Red Maasai and Dorper lambs to evaluate the effects of infection with the gastrointestinal nematode (GIN) Haemonchus contortus on feed intake, liveweight gain (LWG), feed energy and nitrogen partitioning, and enteric methane (CH4) emissions. Six- to seven-month-old Red Maasai (n=12) and Dorper (n=12) lambs were randomly allocated to three treatments (n=8, four lambs per breed) in a 2×3 factorial cross-over study over two periods (P1 and P2) of 36 days each. The treatments consisted of three combinations of GIN infection and feeding level: Infected + ad libitum feeding (I-adlib), uninfected + ad libitum feeding (Un-adlib), and uninfected + restricted feeding (Un-restd), across the two breeds. Lambs in the I-adlib group were trickle-infected daily with 1,000 L3 stage larvae of H. contortus for four consecutive days (Days 1–4), whereas lambs in the other experimental treatments were kept GIN free. The feed intake was measured daily. Liveweight (LW), faecal egg counts (FEC), and packed cell volume (PCV) were measured on Day 1 and weekly thereafter. On Days 29–33 total faecal and urine outputs were determined in metabolic crates. The lambs were then housed in respiration chambers for three consecutive days (Days 34–36). There was a washout period of 21 days before P2 started. Uninfected lambs (Un-adlib and Un-restd) had undetectable FEC throughout the study. On Day 36, FEC did not differ between the breeds (P>0.05). Infected lambs (I-adlib) had lower PCV than uninfected (Un-adlib and Un-restd) lambs on day 36. Neither breed nor infection influenced feed and nutrient intake, but as expected, restricted-fed lambs had a lower intake (P<0.05). The LWG of Un-adlib lambs was significantly higher than that of I-adlib and Un-restd lambs (P<0.05), whereas there was no breed difference (P>0.05). Neither breed nor infection affected feed digestibility, nitrogen retention or energy metabolisability (P>0.05). However, feed restriction decreased feed intake, LWG and N retention, whereas feed digestibility and energy metabolisability were unaffected. Neither daily CH4 emissions nor yield (per unit of feed intake) were affected by experimental infection, but Un-restd lambs had lower CH4 emissions per day. Red Maasai lambs had consistently lower daily CH4 emissions and yields than Dorper (P<0.01). This study confirmed the relative resistance of indigenous sheep (Red Maasai) to H. contortus infection, but the increased CH4 emission and yield due to GIN observed in other studies was not confirmed. Further investigations are needed to test whether in environments with multiple stress factors, local or indigenous breeds or their crossbreeds with exotic breeds may be better equipped to sustain production and simultaneously have a reduced carbon footprint than purebred exotic breeds.

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Section: Effects Of Gin Infection and Breed On Daily Methane Emission...contrasting

confidence: 99%

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...contrasting

confidence: 99%

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...mentioning

confidence: 88%

Section: Effects Of Gin Infection and Breed On Daily Methane Emission...mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 94%

See 3 more Smart Citations

Impact of Haemonchus contortus infection on feed intake, digestion, liveweight gain, and enteric methane emission from Red Maasai and Dorper sheep

Mwangi¹,

Eckard²,

Gluecks³

et al. 2023

Front. Anim. Sci.

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In vivo study: The effects of Carica pubescens seed extract on the anthelmintic activity, feed digestibility, performance, and clinical parameters of thin-tailed sheep

Baihaqi,

Sofyan,

Suwignyo

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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This research serves to follow up on the results of an in vivo study of the administration of aqueous seed extract (ASE) of Carica pubescens waste on the performance and health parameters of thin-tailed sheep. This research was conducted at Al Baihaqi farm in Kediri, East Java. Thirty-two thin-tailed sheep aged 12 months were randomly given four treatments with P0 = negative control, P1 = 2.5% ASE, P2 = 5% ASE, and P4 = positive control (Albendazole). This study used sheep that were naturally exposed to Haemonchus contortus (from the farmer’s field) with the epg qualification being above 1000. The in vivo study was carried out for 9 weeks with the administration of Carica seed extract on the initial 7 days in a row, and the positive control was given Albendazole once on the seventh day. The number of ASE grants was based on the average fluid in the abomasum per kg of body weight. The feed design used in this study was wheat bran and Pennisetum purpureum cv. Mott according to the livestock’s needs. The data taken in this study were the feed consumption, digestibility, average daily gain, percent fecal egg count reduction, body condition score, and clinical parameters. The results showed that the FECR, ADG, and clinical parameters (AST and ALT) (P>0.05) underwent significant changes to support good performance while the BUN, creatinine, feed consumption, and digestibility were normal. This study indicates that ASE of up to 5% can be used as an anthelmintic alternative and support the recovery of sheep performance.

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Interaction between methanotrophy and gastrointestinal nematodes infection on the rumen microbiome of lambs

Corrêa,

Fernandes,

Jimenez

et al. 2024

FEMS Microbiology Ecology

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Complex crosstalk occurs between gastrointestinal nematodes and gut symbiotic microbiota, with consequences for animal metabolism. To investigate the connection between methane production and endoparasites, this study evaluated the effect of mixed infection with Haemonchus contortus and Trichostrongylus colubriformis on methanogenic and methanotrophic community in rumen microbiota of lambs using shotgun metagenomic and Real-time quantitative PCR (qPCR). The rumen content was collected from 6 Santa Inês lambs, (7 months old) before and after 42 days infection by esophageal tube. The metagenomic analysis showed that the infection affected the microbial community structure leading to decreased abundance of methanotrophs bacteria, i.e. α-proteobacteria and β-proteobacteria, anaerobic methanotrophic archaea (ANME), protozoa, sulfate-reducing bacteria, syntrophic bacteria with methanogens, geobacter, and genes related to pyruvate, fatty acid, nitrogen, and sulfur metabolisms, ribulose monophosphate cycle and Entner-Doudoroff Pathway. Additionally, the abundance of methanogenic archaea and the mcrA gene did not change. The co-occurrence networks enabled us to identify the interactions between each taxon in microbial communities and to determine the reshaping of rumen microbiome associations by gastrointestinal nematode infection. Besides, the correlation between ANMEs was lower in the animal's post-infection. Our findings suggest that gastrointestinal parasites potentially lead to decreased methanotrophic metabolism-related microorganisms and genes.

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Hematological, biochemical alterations and methane production in sheep submitted to mixed infection of Haemonchus contortus and Trichostrongylus colubriformis

Cited by 6 publications

References 18 publications

Impact of Haemonchus contortus infection on feed intake, digestion, liveweight gain, and enteric methane emission from Red Maasai and Dorper sheep

Impact of Haemonchus contortus infection on feed intake, digestion, liveweight gain, and enteric methane emission from Red Maasai and Dorper sheep

In vivo study: The effects of Carica pubescens seed extract on the anthelmintic activity, feed digestibility, performance, and clinical parameters of thin-tailed sheep

Interaction between methanotrophy and gastrointestinal nematodes infection on the rumen microbiome of lambs

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