Hematological and histopathological changes in Rattus norvegicus (Wistar) experimentally infected by Angiostrongylus cantonensis (Chen, 1935)

Garcia, Juberlan Silva; Bonfim, Tatiane Cristina dos Santos; Maldonado, Arnaldo; Tunholi, Victor Menezes; Tunholi-Alves, Vinícius Menezes; Mota, Esther Maria; Simões, Raquel de Oliveira; Santana, André Campos; Hooper, Cleber; Pinheiro, Jairo; Bóia, Márcio Neves

doi:10.1016/j.parint.2014.04.008

Cited by 7 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Female worms lay eggs, that embolize to the distal pulmonary arteries (Mackerras and Sandars, 1955). Upon hatching from egg nests, first-stage larvae (L 1 ) penetrate the alveolar walls and induce granulomatous inflammation in the pulmonary interstitium and alveolar spaces, becoming enclosed by loosely-structured granulomas from 45 days post-infection (Hsu et al, 2005;Garcia et al, 2014). By 90 days post-infection, granulomas become fibrotic, leading to alveolar destruction and pulmonary fibrosis (Hsu et al, 2005;Tu and Lai, 2006).…”

Section: Introductionmentioning

confidence: 99%

Gross, microscopic, radiologic, echocardiographic and haematological findings in rats experimentally infected withAngiostrongylus cantonensis

Wun

Davies²,

Spielman

et al. 2020

Parasitology

View full text Add to dashboard Cite

Although the gross and microscopic pathology in rats infected with Angiostrongylus cantonensis has been well described, corresponding changes detected using diagnostic imaging modalities have not been reported. This work describes the cardiopulmonary changes in mature Wistar rats chronically infected with moderate burdens of A. cantonensis using radiology, computed tomography (CT), CT angiography, echocardiography, necropsy and histological examinations. Haematology and coagulation studies were also performed. Thoracic radiography, CT and CT angiography showed moderately severe alveolar pulmonary patterns mainly affecting caudal portions of the caudal lung lobes and associated dilatation of the caudal lobar pulmonary arteries. Presumptive worm profiles could be detected using echocardiography, with worms seen in the right ventricular outflow tract or straddling either the pulmonary and/or the tricuspid valves. Extensive, multifocal, coalescing dark areas and multiple pale foci affecting the caudal lung lobes were observed at necropsy. Histologically, these were composed of numerous large, confluent granulomas and fibrotic nodules. Adult worms were found predominantly in the mid- to distal pulmonary arteries. An inflammatory leukogram, hyperproteinaemia and hyperfibrinogenaemia were found in most rats. These findings provide a comparative model for A. cantonensis in its accidental hosts, such as humans and dogs. In addition, the pathological and imaging changes are comparable to those seen in dogs infected with Angiostrongylus vasorum, suggesting rats infected with A. cantonensis could be a model for dogs with A. vasorum infection.

show abstract

Section: Introductionmentioning

confidence: 99%

Gross, microscopic, radiologic, echocardiographic and haematological findings in rats experimentally infected withAngiostrongylus cantonensis

Wun

Davies²,

Spielman

et al. 2020

Parasitology

View full text Add to dashboard Cite

show abstract

“…Infection of the definitive host R. norvegicus by A. cantonesis has a 10 – 20% mortality and causes normocytic hypochromic anemia, thrombocytopenia, eosinophilia, neutrophilia, basophilia, increased cardiac enzymes, acidosis and hypoxia that are associated with necrosis and fibrosis of the lung parenchyma (Garcia et al, 2014). Mice, like humans, are non-permissive hosts developing extensive brain injury and neurological symptoms such as ataxia, tremor and paralysis when infected (OuYang et al, 2012).…”

Section: Epidemiologymentioning

confidence: 99%

Central nervous system manifestations of Angiostrongylus cantonensis infection

2015

View full text Add to dashboard Cite

Over 20 species of Angiostrongylus have been described from around the world, but only Angiostrongylus cantonensis has been confirmed to cause central nervous system disease in humans. A neurotropic parasite that matures in the pulmonary arteries of rats, A. cantonensis is the most common cause of eosinophilic meningitis in southern Asia and the Pacific and Caribbean islands. The parasite can also cause encephalitis/encephalomyelitis and rarely ocular angiostrongyliasis. The present paper reviews the life cycle, epidemiology, pathogenesis, clinical features, diagnosis, treatment, prevention and prognosis of A. cantonesis infection. Emphasis is given on the spectrum of central nervous system manifestations and disease pathogenesis.

show abstract

“…, Viney and Kikuchi ) and can reduce rat body condition through anemia and tissue damage (Garcia et al. , Viney and Kikuchi ). More specific body condition loss can also occur.…”

Section: Introductionmentioning

confidence: 99%

Coinfection modifies carriage of enzootic and zoonotic parasites in Norway rats from an urban slum

et al. 2019

View full text Add to dashboard Cite

We examine coinfection between helminth species and the bacterium Leptospira interrogans in a natural population of the Norway rat, Rattus norvegicus. We ask whether coinfection can influence the probability and intensity of infection of these enzootic and zoonotic parasites in urban rats, which may affect the loads of parasites excreted into the environment. Rodent sampling was carried out during two seasons in 2014 in a Brazilian urban slum. We sampled rats’ feces, kidney imprints, and urine to identify and quantify helminth eggs/larvae and infection by L. interrogans. Eleven species/groups of helminths and L. interrogans were identified among 299 captured rats. Simple correlation tests and generalized linear models were performed to identify general patterns of coinfection and potential direction of effects, respectively, after controlling for consolidated environmental and host biotic variables. Significant associations were illustrated in an interaction network. Focusing on parasites with the potential to cause zoonoses among humans, we observed that coinfection between L. interrogans and the nematode Angiostrongylus cantonensis was significantly more frequent than expected. Reduced prevalence of A. cantonensis was found in the presence of Nippostrongylus brasiliensis, and N. brasiliensis intensities (eggs per gram of feces) were increased with greater intensities of both L. interrogans and Strongyloides sp., the latter of which was, in turn, found to increase the intensities of A. cantonensis. A higher probability of finding L. interrogans was associated with infection by Strongyloides sp. Our study provides a novel perspective on evaluating helminth coinfection profiles in populations of naturally infected urban rats, moving beyond previous studies which have been limited to descriptions of co‐occurrence. Noticeably, infection risk was dependent on coinfection and this should be accounted for when targeting the control of zoonotic pathogens in natural populations.

show abstract

Hematological and histopathological changes in Rattus norvegicus (Wistar) experimentally infected by Angiostrongylus cantonensis (Chen, 1935)

Cited by 7 publications

References 32 publications

Gross, microscopic, radiologic, echocardiographic and haematological findings in rats experimentally infected withAngiostrongylus cantonensis

Gross, microscopic, radiologic, echocardiographic and haematological findings in rats experimentally infected withAngiostrongylus cantonensis

Central nervous system manifestations of Angiostrongylus cantonensis infection

Coinfection modifies carriage of enzootic and zoonotic parasites in Norway rats from an urban slum

Contact Info

Product

Resources

About