Hematological and Biochemical Parameters in Apparently Healthy Indian Population: Defining Reference Intervals

Sairam, Shrilekha; Domalapalli, Suhasini; Maruthamuthu, S.; Swaminathan, J.; Ramesh, Vivek A.; Sekhar, Lalitha; Pandeya, Palak; Balasubramaniam, Udhaya

doi:10.1007/s12291-013-0365-5

Cited by 40 publications

(32 citation statements)

References 21 publications

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“…In another Indian study, the AST reference ranges were reported by Sairam et al for reference population comprising of four Indian cities (Chennai, Hyderabad, New Delhi and Ahmadabad). The RI established in this study for AST was-(12.0-37.0 IU/L for female and 14.0-42.0 IU/L for male) [22]. The RIs of GGT calculated in our study was 7.9-34.6 IU/L for female and 8.6-36.2 IU/L for male.…”

Section: Discussionsupporting

confidence: 49%

Hepatic Enzyme’s Reference Intervals and Their Modulating Factors in Western Indian Population

Maksane¹,

Dandekar²,

Shukla

et al. 2015

Ind J Clin Biochem

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The reference intervals (RIs) of serum aminotransferases and Gamma-glutamyl transferase (GGT) have been established many years ago. Recent RIs are not available. The prospective study was conducted to re-evaluate the RIs of liver enzymes and the effect of demographic and anthropometric variables on them in western Indian population. A total of 1059 blood donors comprised the study population. Anthropometry and serum liver enzymes levels were measured. Subjects were categorized into normal weight and overweight by using body mass index (BMI) and waist circumference (WC). For RI determination, non-parametric methodology recommended by IFCC/CLSI was adopted. Mann-Whitney test and Spearman's rank correlation were used for statistical analysis. Upper limit of normal reference value of liver enzymes were lower in female compared to male. (ALT-23.55 F vs 36.00 M, GGT-34.58 F vs 36.20 M) When RI of liver enzymes were calculated according to body mass index, the upper limit of normal of ALT and GGT were higher in overweight group compared to normal weight group. (ALT-38.00 vs 27.00 IU/L and GGT-37.59 vs 35.26 IU/L). In both male and female, liver enzymes correlated significantly with age. WC and BMI were positively correlated with AST, ALT and GGT in both subgroups and the correlation was stronger in male. Demographic factors should be considered for making liver enzyme tests more clinically relevant. Gender based partitioning should be adopted for serum alanine aminotransferase (ALT) and GGT reference values for Western Indian population.

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Section: Discussionsupporting

confidence: 49%

Hepatic Enzyme’s Reference Intervals and Their Modulating Factors in Western Indian Population

Maksane¹,

Dandekar²,

Shukla

et al. 2015

Ind J Clin Biochem

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“…Compared to other African countries, the total cholesterol reference ranges were similar to one derived from Ivory Coast [ 1 ], Kenya [ 7 ], Tanzania [ 7 ] and Mozambique [ 16 ]. On the other hand, total cholesterol levels in Burkina were higher than those from Benin[ 4 ], Ghana [ 17 ] and Nigeria [ 5 ].Compared to Indian values, total cholesterol values were close to those of Goswami [ 18 ] and Gupta[ 6 ] but lower than those of Madhumita [ 8 ] and Sairam [ 19 ]. Our values were lower than those recorded in France [ 20 ] and in USA [ 7 ].…”

Section: Discussionmentioning

confidence: 67%

Reference Ranges of Cholesterol Sub-Fractions in Random Healthy Adults in Ouagadougou, Burkina Faso

Koumaré

Sakandé²,

Kabré

et al. 2015

PLoS ONE

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In Burkina Faso, the values that serve as clinical chemistry reference ranges are those provided by European manufacturers’ insert sheets based on reference of the Western population. However, studies conducted so far in some African countries reported significant differences in normal laboratory ranges compared with those of the industrialized world. The aim of this study was to determine reference values of cholesterol fractions in apparently normal adults in Burkina Faso that could be used to better assess the risks related to cardiovascular diseases. Study population was 279 healthy subjects aged from 15 to 50 years including 139 men and 140 women recruited at the Regional Center of Blood Transfusion of Ouagadougou, capital city of Burkina Faso (West Africa). Exclusion criteria based on history and clinical examination were used for defining reference individuals. The dual-step precipitation of HDL cholesterol sub-fractions using dextran sulfate was performed according to the procedure described by Hirano. The medians were calculated and reference values were determined at 2.5th and 97.5th percentiles. The median and upper ranges for total cholesterol, LDL cholesterol, total HDL cholesterol and HDL2 cholesterol were observed to be higher in women in comparison to men (p <0.05). These reference ranges were similar to those derived from other African countries but lower than those recorded in France and in USA. This underscores the need for such comprehensible establishment of reference values for limited resources countries. Our study provides the first cholesterol sub-fractions (HDL2 and HDL3) reference ranges for interpretation of laboratory results for cardiovascular risk management in Burkina Faso.

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“…The levels of serum total protein, globulin, and the A/G ratio were greater than the lower boundary 95% CI for AUC >0.5 and found to be significant. The cutoff values of serum proteins used were the reference intervals for healthy Indian males as reported by Sairam et al [21]. The optimal cut point values for BLL in serum proteins are presented in Table 4.…”

Section: Resultsmentioning

confidence: 99%

Assessment of diagnostic accuracy and optimal cut point of blood lead levels on serum proteins among Pb-exposed workers from Pb-battery plant.

Kalahasthi¹

2019

Int J Med Biochem

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Assessment of diagnostic accuracy and optimal cut points of blood lead levels on serum proteins among workers exposed to Pb at a lead battery plant L ead (Pb)-binding proteins originate in the kidney, brain, erythrocytes, liver, and lung [1]. Proteins related to heme synthesis, calcium metabolism, and neurotransmission are associated with Pb poisoning [2]. Systemic effects of hypertension, frank anemia, cognitive deficits, infertility, immune imbalances, delayed skeletal and deciduous dental develop-Objectives: This study assessed the diagnostic accuracy and the optimal cut point of blood lead (Pb) level (BLL) in serum proteins (total protein, albumin, globulin, and albumin/globulin ratio) among workers with exposure to Pb at a Pb battery plant. Methods: An examination of the diagnostic accuracy and optimal cut point value of BLL in serum proteins among workers exposed to Pb was performed using analysis of the area under the curve (AUC) and coordinates of receiving operating characteristic (ROC) curve. The study group consisted of 176 males who worked in a Pb battery plant. A control group consisted of 80 male office workers with no unusual occupational Pb exposure. The BLL of the patients was assessed using an atomic absorption spectrophotometer. The serum protein levels (total protein and albumin) were determined using diagnostic kits. The serum globulin was calculated from the total protein and albumin measures. The albumin:globulin ratio (A/G ratio) was calculated from the albumin and globulin levels. Results: The serum total protein and globulin concentration values were lower and the A/G ratio was significantly higher in the study group compared with the control group. A negative and significant association was found between the BLL and serum total protein and serum globulin values. A positive and significant association was found between the BLL and the serum albumin level and the A/G ratio. The AUC value was 0.626 (95% confidence interval [CI]: 0.540-0.712; p=0.005) for serum total protein with a cutoff value of ≤6.9 g/dL. For serum albumin, the AUC value was 0.466 (95% CI: 0.344-0.587; p=0.574) with a cutoff value of ≤4.0 g/dL. The AUC value of serum globulin was 0.625 (95% CI: a0.541-0.708, p=0.005) with a cutoff value of ≤2.4g/dL. The A/G ratio had an AUC value of 0.606 (95% CI: 0.521-0.692; p=0.012) with a cutoff value >1.5g/dL. An optimal BLL cut point value of 25.5µg/dL was noted for serum total protein, with 56% sensitivity and 51% specificity. The BLL value of 24.5 µg/dL was determined to be the optimal cut point for serum albumin, with 44% sensitivity and 44% specificity. The use of 27.5 µg/dL as an optimal BLL cut point value for serum globulin and the A/G ratio demonstrated 55% sensitivity and 55% specificity. Conclusion: The AUC values of serum total protein, globulin, and the A/G ratio were found to be significant when compared with the serum albumin. BLL optimal cut point values for serum proteins in Pb exposure ranged from 24.5 µg/dL to 27.5 µg/dL. The change in serum total protein, globuli...

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Hematological and Biochemical Parameters in Apparently Healthy Indian Population: Defining Reference Intervals

Cited by 40 publications

References 21 publications

Hepatic Enzyme’s Reference Intervals and Their Modulating Factors in Western Indian Population

Hepatic Enzyme’s Reference Intervals and Their Modulating Factors in Western Indian Population

Reference Ranges of Cholesterol Sub-Fractions in Random Healthy Adults in Ouagadougou, Burkina Faso

Assessment of diagnostic accuracy and optimal cut point of blood lead levels on serum proteins among Pb-exposed workers from Pb-battery plant.

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